Skip to main content
SpringerLink
Log in

Axon Regeneration

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Ava J. Udvadia

    1. Department of Biology, Appalachian State University, Boone, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. James B. Antczak

    1. Office of Technology Development, Medical College of Wisconsin, Milwaukee, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2636)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both in vivo and in vitro approaches, physical injury models, methods for the isolation/analysis of various macromolecules, live and fixed imaging of regenerating axons, and for quantifying behavioral endpoints enable measurements of regenerative success. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Axon Regeneration: Methods and Protocols aims to be comprehensive guide for researchers.

Similar content being viewed by others

Keywords

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Defining Selective Neuronal Resilience and Identifying Targets for Neuroprotection and Axon Regeneration Using Single-Cell RNA Sequencing: Experimental Approaches

    • Anne Jacobi, Nicholas M. Tran
    Pages 1-18

  3. Defining Selective Neuronal Resilience and Identifying Targets of Neuroprotection and Axon Regeneration Using Single-Cell RNA Sequencing: Computational Approaches

    • Salwan Butrus, Srikant Sagireddy, Wenjun Yan, Karthik Shekhar
    Pages 19-41

  4. Retinal Ganglion Cell Axon Fractionation

    • Sean D. Meehan, Sanjoy Bhattacharya
    Pages 43-53

  5. Analysis of Immediate Early Gene Expression Levels to Interrogate Changes in Cortical Neuronal Activity Patterns upon Vision Loss

    • Sara R. J. Gilissen, Maroussia Hennes, Lutgarde Arckens
    Pages 55-70

  6. Noncoding Regulatory RNAs: Isolation and Analysis of Neuronal Circular RNAs and MicroRNAs

    • Michela Dell’Orco, Grigorios Papageorgiou, Nikolaos Mellios, Nora I. Perrone-Bizzozero
    Pages 71-83

  7. Transneuronal Delivery of Cytokines to Stimulate Mammalian Spinal Cord Regeneration

    • Daniel Terheyden-Keighley, Marco Leibinger, Charlotte Zeitler, Dietmar Fischer
    Pages 85-99

  8. Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury

    • Franziska Müller, Jessica S. Chadwick, Simone Di Giovanni, Ilaria Palmisano
    Pages 101-144

  9. Profiling Locally Translated mRNAs in Regenerating Axons

    • Pabitra K. Sahoo, Jeffery L. Twiss
    Pages 145-161

  10. Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish

    • An Beckers, Steven Bergmans, Annelies Van Dyck, Lieve Moons
    Pages 163-190

  11. Assaying Optic Nerve Regeneration in Larval Zebrafish

    • Beth M. Harvey, Melissa Baxter, Michael Granato
    Pages 191-203

  12. Surgical Methods in Postmetamorphic Xenopus laevis: Optic Nerve Crush Injury Model

    • Alexis M. Feidler, Hieu H. M. Nguyen, Fiona L. Watson
    Pages 205-219

  13. Generating Widespread and Scalable Retinal Lesions in Adult Zebrafish by Intraocular Injection of Ouabain

    • Diana M. Mitchell, Deborah L. Stenkamp
    Pages 221-235

  14. A Reproducible Spinal Cord Crush Injury in the Regeneration-Permissive Axolotl

    • Sarah Walker, Tiago Santos-Ferreira, Karen Echeverri
    Pages 237-246

  15. Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish

    • Kadidia P. Adula, Alvaro Sagasti
    Pages 247-261

  16. Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish

    • Louisa K. Drake, Marcus Keatinge, Themistoklis M. Tsarouchas, Catherina G. Becker, David A. Lyons, Thomas Becker
    Pages 263-277

  17. Translating Ribosome Affinity Purification (TRAP) and Bioinformatic RNA-Seq Analysis in Post-metamorphic Xenopus laevis

    • Gregg B. Whitworth, Fiona L. Watson
    Pages 279-310

  18. LCM-Seq for Retinal Cell Layer-Specific Responses During Optic Nerve Regeneration

    • Wesley Speer, Matthew B. Veldman
    Pages 311-321

  19. Profiling Dynamic Changes in DNA Accessibility During Axon Regeneration After Optic Nerve Crush in Adult Zebrafish

    • Sumona P. Dhara, Ava J. Udvadia
    Pages 323-341

  20. Quantitative Proteomics of Nervous System Regeneration: From Sample Preparation to Functional Data Analyses

    • Dasfne Lee-Liu, Liangliang Sun
    Pages 343-366
Back to top

Editors and Affiliations

  • Department of Biology, Appalachian State University, Boone, USA

    Ava J. Udvadia

  • Office of Technology Development, Medical College of Wisconsin, Milwaukee, USA

    James B. Antczak

Bibliographic Information

  • Book Title: Axon Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Ava J. Udvadia, James B. Antczak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3012-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3011-2Published: 08 March 2023

  • Softcover ISBN: 978-1-0716-3014-3Published: 08 March 2024

  • eBook ISBN: 978-1-0716-3012-9Published: 07 March 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 451

  • Number of Illustrations: 5 b/w illustrations, 65 illustrations in colour

  • Topics: Neurosciences

Publish with us

Policies and ethics

Back to top

Search

Navigation