Abstract
In addition to expressing a large number of protein-coding transcripts, including alternatively spliced isoforms of the same mRNAs, neurons express a large number of noncoding RNAs. These include microRNAs (miRNAs), circular RNAs (circRNAs), and other regulatory RNAs. The isolation and quantitative analyses of diverse types of RNAs in neurons are critical to understand not only the posttranscriptional mechanisms regulating mRNA levels and their translation but also the potential of several RNAs expressed in the same neurons to regulate these processes by generating networks of competing endogenous RNAs (ceRNAs). This chapter will describe methods for the isolation and analyses of circRNA and miRNA levels from the same brain tissue sample.
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Acknowledgments
This work was supported by NIH, R01DA034097, and R21 DA048651 grants to N.P.B. as well as a mentored PI grant as part of an P20 grant from the NIGMS (1P20GM121176-01, N.M.), a NIAAA R01 grant (R01AA026583, N.M. and G.P.), a NARSAD Young Investigator Grant (FP00000839, N.M.) by the Brain & Behavior Research Foundation, and a high priority short-term R56 award from the NIMH (1R56MH119150-01, N.M.).
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Dell’Orco, M., Papageorgiou, G., Mellios, N., Perrone-Bizzozero, N.I. (2023). Noncoding Regulatory RNAs: Isolation and Analysis of Neuronal Circular RNAs and MicroRNAs. In: Udvadia, A.J., Antczak, J.B. (eds) Axon Regeneration. Methods in Molecular Biology, vol 2636. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3012-9_5
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DOI: https://doi.org/10.1007/978-1-0716-3012-9_5
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