Abstract
Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.
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Acknowledgments
This work was supported by the EU Cofund ERANET NEURON consortium NEURONICHE with contributions from MRC (MR/R001049/1), Spinal Research, and Wings for Life to CGB, a project grant from the BBSRC (BB/R003742/1) to TB, by a Wellcome Trust Senior Research Fellowship (102836/Z/13/Z) to DAL and by Biogen who provided funding via a scientific research agreement with DAL. LKD was funded by a BBSRC Eastbio PhD studentship.
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Drake, L.K., Keatinge, M., Tsarouchas, T.M., Becker, C.G., Lyons, D.A., Becker, T. (2023). Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish. In: Udvadia, A.J., Antczak, J.B. (eds) Axon Regeneration. Methods in Molecular Biology, vol 2636. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3012-9_15
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DOI: https://doi.org/10.1007/978-1-0716-3012-9_15
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Online ISBN: 978-1-0716-3012-9
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