Abstract
RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), and assay for transposase-accessible chromatin sequencing (ATAC-seq) are genome-wide techniques that provide information relative to gene expression, chromatin binding sites, and chromatin accessibility, respectively. Here we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy, to characterize the transcriptional and epigenetic signatures of DRG upon regenerative vs non-regenerative axonal lesion.
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Acknowledgments
The work has been supported by the Wings for Life (SDG), Rosetrees Trust (SDG), Leverhulme Trust (SDG), International Spinal Research Trust (SDG), Brain Research UK (SDG), and Medical Research Council (SDG). The schematics have been generated using BioRender (BioRender.com).
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Müller, F., Chadwick, J.S., Di Giovanni, S., Palmisano, I. (2023). Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury. In: Udvadia, A.J., Antczak, J.B. (eds) Axon Regeneration. Methods in Molecular Biology, vol 2636. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3012-9_7
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DOI: https://doi.org/10.1007/978-1-0716-3012-9_7
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