Overview
- Includes cutting-edge lab techniques
- Provides step-by-step detail essential for reproducible results
- Contains key notes and advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1886)
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About this book
This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.
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Keywords
Table of contents (21 protocols)
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Imaging
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Single-Molecule Force Spectroscopy
Editors and Affiliations
Bibliographic Information
Book Title: Atomic Force Microscopy
Book Subtitle: Methods and Protocols
Editors: Nuno C. Santos, Filomena A. Carvalho
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8894-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8893-8Published: 30 October 2018
Softcover ISBN: 978-1-4939-9407-6Published: 10 December 2019
eBook ISBN: 978-1-4939-8894-5Published: 29 October 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 372
Number of Illustrations: 16 b/w illustrations, 87 illustrations in colour
Topics: Biological Microscopy