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NMDA Receptors

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Nail Burnashev

    1. INSERM, Institut de Neurobiologie de la Méditerranée (INMED), Marseille, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Pierre Szepetowski

    1. INSERM, Institut de Neurobiologie de la Méditerranée (INMED), Marseille, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2799)

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About this book

This fully updated volume explores N-Methyl-D-aspartate receptors (NMDARs), and the structure-function relations, principles, and rules that govern how NMDARs operate in brain processing under normal and pathological conditions. With chapters from leading laboratories around the world, this book examines the expression and purification of diverse NMDA receptor subtypes, gene targeting and generation of conditional NMDAR mutant mouse lines, studies of NMDARs in zebrafish, electrophysiological studies, NMDARs modeling in silico, drug development with artificial intelligence, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, NMDA Receptors: Methods and Protocols, SecondEdition serves as an ideal guide to the diversity of possible approaches in the field of NMDARs and the progress that has been made in recent years.

Keywords

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Spectrum of NMDA Receptor Variants in Neurodevelopmental Disorders and Epilepsy

    • Cathrine E. Gjerulfsen, Ilona Krey, Chiara Klöckner, Guido Rubboli, Johannes R. Lemke, Rikke S. Møller
    Pages 1-11

  3. Expression and Purification of Mammalian NMDA Receptor Protein for Functional Characterization

    • Xuejing Huang, Wanjin Chen, Shujia Zhu
    Pages 13-27

  4. Analysis of Surface Expression of NMDAR Subunits in Primary Hippocampal Neurons

    • Viktor Kuchtiak, Tereza Smejkalova, Martin Horak, Ladislav Vyklicky, Ales Balik
    Pages 29-46

  5. Transfection in Primary Cultured Neuronal Cells

    • Katie F. M. Marwick, Giles E. Hardingham
    Pages 47-54

  6. Selective Cell-Surface Expression of Triheteromeric NMDA Receptors

    • Feng Yi, Stephen F. Traynelis, Kasper B. Hansen
    Pages 55-77

  7. Generation of Rare Human NMDA Receptor Variants in Mice

    • Rolf Sprengel, Ahmed Eltokhi, Frank N. Single
    Pages 79-105

  8. Behavioral Analysis of NMDAR Function in Rodents: Tests of Long-Term Spatial Memory

    • David M. Bannerman, Chris Barkus, Ahmed Eltokhi
    Pages 107-138

  9. Functional Analysis of NMDAR Subunit Components in Postsynaptic Currents of Identified Cells and Synapses in Brain Slices

    • Louison Pineau, Nail Burnashev
    Pages 139-150

  10. Estimating the Ca2+ Block of NMDA Receptors with Single-Channel Electrophysiology

    • Gary J. Iacobucci, Gabriela K. Popescu
    Pages 151-175

  11. Estimating the Ca2+ Permeability of NMDA Receptors with Whole-Cell Patch-Clamp Electrophysiology

    • Mae G. Weaver, Gabriela K. Popescu
    Pages 177-200

  12. Analysis of Functional NMDA Receptors in Astrocytes

    • Frank Kirchhoff, Wannan Tang
    Pages 201-223

  13. Single-Molecule FRET Analyses of NMDA Receptors

    • Ryan J. Durham, Vasanthi Jayaraman
    Pages 225-242

  14. Behavioral Assays Dissecting NMDA Receptor Function in Zebrafish

    • Josiah D. Zoodsma, Carly I. Gomes, Howard I. Sirotkin, Lonnie P. Wollmuth
    Pages 243-255

  15. Characterizing Functional Contributions of Specific GluN2 Subunits to Individual Postsynaptic NMDAR Responses Using Biophysical Parameters

    • Annemarie Dedek, Michael E. Hildebrand
    Pages 257-267

  16. Modeling and Simulation of the NMDA Receptor at Coarse-Grained and Atomistic Levels

    • Wenjun Zheng, Xing Liu
    Pages 269-280

  17. Application of Deep Learning for Studying NMDA Receptors

    • Zhenfeng Deng, Ruichu Gu, Han Wen
    Pages 281-290

  18. Correction to: Estimating the Ca2+ Permeability of NMDA Receptors with Whole-Cell Patch-Clamp Electrophysiology

    • Mae G. Weaver, Gabriela K. Popescu
    Pages C1-C1

  19. Back Matter

    Pages 291-292
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Editors and Affiliations

  • INSERM, Institut de Neurobiologie de la Méditerranée (INMED), Marseille, France

    Nail Burnashev, Pierre Szepetowski

Bibliographic Information

  • Book Title: NMDA Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Nail Burnashev, Pierre Szepetowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3830-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3829-3Published: 11 May 2024

  • Softcover ISBN: 978-1-0716-3832-3Due: 25 May 2025

  • eBook ISBN: 978-1-0716-3830-9Published: 10 May 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 292

  • Number of Illustrations: 2 b/w illustrations, 54 illustrations in colour

  • Topics: Neurochemistry, Neurosciences

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