Abstract
Transfection allows the introduction of foreign nucleic acid into eukaryotic cells. It is an important tool in understanding the roles of NMDARs in neurons. Here we describe using lipofection-mediated transfection to introduce cDNA encoding NMDAR subunits into postmitotic rodent primary cortical neurons maintained in culture.
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Acknowledgments
This work was supported by a Wellcome Trust Clinical PhD studentship grant to KFMM. Much of the work put into optimizing this protocol was done by others in GEH’s lab over the last decade, particularly, Sofia Papadia and Sean McKay. Michael Greenberg’s lab developed the principles of transfection in neurons. Jonathan Shutt designed Fig. 2.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Marwick, K.F.M., Hardingham, G.E. (2024). Transfection in Primary Cultured Neuronal Cells. In: Burnashev, N., Szepetowski, P. (eds) NMDA Receptors. Methods in Molecular Biology, vol 2799. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3830-9_4
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DOI: https://doi.org/10.1007/978-1-0716-3830-9_4
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