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Histone Methyltransferases

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Raphaël Margueron

    1. Institute Curie, INSERM U934/CNRS UMR 3215, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Daniel Holoch

    1. Institute Curie, INSERM U934/CNRS UMR 3215, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2529)

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About this book

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.


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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Introduction and Biological Context

    1. Front Matter

      Pages 1-1
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    2. Not all Is SET for Methylation: Evolution of Eukaryotic Protein Methyltransferases

      • Allyson A. Erlendson, Michael Freitag
      Pages 3-40

  3. In Vitro Enzymatic Assays

    1. Front Matter

      Pages 41-41
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    2. Detection and Quantification of Histone Methyltransferase Activity In Vitro

      • Nwamaka J. Idigo, Philipp Voigt
      Pages 43-61

    3. In Vitro Histone Demethylase Assays

      • Shengjiang Tu
      Pages 63-88

  4. Exploring the Regulation of Histone Methyltransferase Enzymatic Activity

    1. Front Matter

      Pages 89-89
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    2. Preparation and Characterization of Chromatin Templates for Histone Methylation Assays

      • Cuifang Liu, Jicheng Zhao, Guohong Li
      Pages 91-107

    3. Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact

      • Luis Popoca, Chul-Hwan Lee
      Pages 109-120

    4. Profiling the Regulation of Histone Methylation and Demethylation by Metabolites and Metals

      • Sebastian Müller, Fabien Sindikubwabo, Tatiana Cañeque, Raphaël Rodriguez
      Pages 121-133

  5. Structural Investigation of Histone Methyltransferases

    1. Front Matter

      Pages 135-135
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    2. Determination of Histone Methyltransferase Structure by Crystallography

      • Jon R. Wilson
      Pages 137-147

    3. Determination of Histone Methyltransferase Structures in Complex with the Nucleosome by Cryogenic Electron Microscopy

      • Cathy J. Spangler, Robert K. McGinty
      Pages 149-168

  6. Analysis of Histone Methylation in Cells and Genomes

    1. Front Matter

      Pages 169-169
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    2. Development and Validation of Antibodies Targeting Site-Specific Histone Methylation

      • Lara Zorro Shahidian, Sylvain Daujat
      Pages 171-206

    3. Genetic, Genomic, and Imaging Approaches to Dissect the Role of Polycomb Group Epigenetic Regulators in Mice

      • Nayuta Yakushiji-Kaminatsui, Takashi Kondo, Yasuhide Ohinata, Junichiro Takano, Haruhiko Koseki
      Pages 207-228

    4. Profiling Histone Methylation in Low Numbers of Cells

      • Julie Brind’Amour, Matthew C. Lorincz
      Pages 229-251

    5. Automated CUT & RUN Using the KingFisher Duo Prime

      • Setareh Aflaki, Raphaël Margueron, Daniel Holoch
      Pages 253-265

    6. Bioinformatics Methods for ChIP-seq Histone Analysis

      • Nicolas Servant
      Pages 267-293

  7. Discovery of Histone Methyltransferase Substrates and Methylated Histone Interactors

    1. Front Matter

      Pages 295-295
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Editors and Affiliations

  • Institute Curie, INSERM U934/CNRS UMR 3215, Paris, France

    Raphaël Margueron, Daniel Holoch

Bibliographic Information

  • Book Title: Histone Methyltransferases

  • Book Subtitle: Methods and Protocols

  • Editors: Raphaël Margueron, Daniel Holoch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2481-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2480-7Published: 24 June 2022

  • Softcover ISBN: 978-1-0716-2483-8Published: 25 June 2023

  • eBook ISBN: 978-1-0716-2481-4Published: 22 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 495

  • Number of Illustrations: 19 b/w illustrations, 53 illustrations in colour

  • Topics: Enzymology, Biological Techniques, Genetics and Genomics

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