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Development and Validation of Antibodies Targeting Site-Specific Histone Methylation

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Histone Methyltransferases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2529))

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Abstract

The development of specific anti-modification antibodies as research tools has revolutionized the way histone methylation is studied. Lack of stringent quality controls, however, led to the development of nonspecific antibodies, compromising their use. In this chapter, we provide a series of protocols that collectively will help those studying histone methylation to develop and thoroughly validate high-end sequence-specific and methylation-dependent antibodies.

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Acknowledgments

We would like to thank Robert Schneider for his support and people from his laboratory for helpful discussions. We would like to thank Andrew Bannister for critical reading an earlier version of the manuscript. This work was supported by the Agence Nationale de la Recherche (CoreAc).

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Authors and Affiliations

  1. Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), University of Cantabria, Santander, Spain

    Lara Zorro Shahidian

  2. Biotechnology and Cell Signaling, CNRS UMR7242, University of Strasbourg, Illkirch Cedex, France

    Sylvain Daujat

Authors
  1. Lara Zorro Shahidian
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  2. Sylvain Daujat
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Corresponding author

Correspondence to Sylvain Daujat .

Editor information

Editors and Affiliations

  1. Institute Curie, INSERM U934/CNRS UMR 3215, Paris, France

    Raphaël Margueron

  2. Institute Curie, INSERM U934/CNRS UMR 3215, Paris, France

    Daniel Holoch

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zorro Shahidian, L., Daujat, S. (2022). Development and Validation of Antibodies Targeting Site-Specific Histone Methylation. In: Margueron, R., Holoch, D. (eds) Histone Methyltransferases. Methods in Molecular Biology, vol 2529. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2481-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2481-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2480-7

  • Online ISBN: 978-1-0716-2481-4

  • eBook Packages: Springer Protocols

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