Abstract
In eukaryotic cells, chromatin plays an important role in gene regulation by controlling the access of the transcription machinery to DNA. In this chapter, we will describe methods for generating different chromatin templates to investigate the impact of histone variants and chromatin structure on histone methyltransferase activities. For this purpose, we take Polycomb Repressive Complex 2 (PRC2) as an example and investigate how its activity on H3K27me3 is regulated by the histone variants H3.3 and H2A.Z and higher-order chromatin structure.
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Acknowledgments
This work was supported by grants from the Ministry of Science and Technology of China (2017YFA0504202 to G.L.; 2019YFA0508903 to J.Z.), the National Natural Science Foundation of China (31991161,31521002 to G.L.; 32070604 to J.C.) and the Beijing Municipal Science and Technology Committee (Z201100005320013 to G.L.). The work was also supported by the CAS Key Research Program on Frontier Science (QYZDY-SSW-SMC020 to G.L.), the Chinese Academy of Sciences (CAS) Strategic Priority Research Program (XDB19040202), an HHMI International Research Scholar grant (55008737) to G.L.
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Liu, C., Zhao, J., Li, G. (2022). Preparation and Characterization of Chromatin Templates for Histone Methylation Assays. In: Margueron, R., Holoch, D. (eds) Histone Methyltransferases. Methods in Molecular Biology, vol 2529. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2481-4_4
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DOI: https://doi.org/10.1007/978-1-0716-2481-4_4
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