Abstract
The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.
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Acknowledgments
We thank Dr. D. Reinberg for critical reading of the manuscript. This work was supported by Research Resettlement Fund for the new faculty of Seoul National University, Creative-Pioneering Researchers Program through Seoul National University, grants from Seoul National University College of Medicine, National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1C1C1013220), and BK21 Four Biomedical Science Program. L. Popoca is supported by National Institutes of Health (NIH) grant R01CA199652, the Howard Hughes Medical Institute (HHMI), and the Making Headway Foundation St. Baldrick’s Research Grant (189290).
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Popoca, L., Lee, CH. (2022). Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact. In: Margueron, R., Holoch, D. (eds) Histone Methyltransferases. Methods in Molecular Biology, vol 2529. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2481-4_5
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DOI: https://doi.org/10.1007/978-1-0716-2481-4_5
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