Abstract
Background
Although phytoplankton are important primary producers in food webs, they are relatively less studied in large rivers compared to other types of systems. To fill this research gap, we studied phytoplankton taxonomic and functional composition and their relationships with water quality, habitat, climate, and land use across 30 river sections in the middle and lower reaches of the Yangtze River during 2017–2018.
Results
Major observed phytoplankton groups were cyanobacteria, bacillariophyta, and chlorophyta. Phytoplankton total abundance, total biomass, and species richness significantly decreased in the dry season compared to the wet season, with the species and functional composition differing significantly between seasons. Phytoplankton species differences between seasons were mainly contributed by Oscillatoria sp., Pseudanabaena sp., and Melosira granulata. The dfferences in phytoplankton functional groups between seasons were mainly contributed by P (including Closterium sp., Melosira sp.), Lo (including Merismopedia sp., Peridinium sp., Ceratium sp., and Gymnodinium sp.), and J (including Pediastrum sp., Tetraedron sp., Crucigenia sp., Scenedesmus sp., and Coelastrum sp.). The variance partitioning showed that water quality (NO3-N, total suspended solids, turbidity) and habitat (water flow, river bank and river channel conditions) were critical factors in shaping phytoplankton patterns, followed by climate and land use.
Conclusions
Results indicated that there was significant seasonal variation of phytoplankton in the Yangtze River, with water quality and habitat primarily driving phytoplankton patterns. Our study contributes to the understanding of natural and anthropogenic factors that drive seasonal successional processes of phytoplankton in the Yangtze River. These findings have important implications for environmental management as well as towards the ecological restoration of large rivers.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Introduction
The world’s great human civilizations have flourished along large rivers (Best 2019; Fang and Jawitz 2019). Rivers are one of the most dynamic ecosystems known, offering a variety of ecological services to life on earth and being crucial to human well-being across the planet (Chen et al. 2016; Xiong et al. 2021). Multiple anthropogenic disturbances have significant impacts on riverine ecosystems (Birk 2019; Qu et al. 2022; Xiong et al. 2022), which is partly why these ecosystems have among the highest rates of biodiversity declines of all ecosystems on earth (Dudgeon et al. 2006; Arneth et al. 2020). For example, modification of rivers or floodplains affected river connectivity, which has in turn led to large declines in biodiversity (Stoffers et al. 2022; Xiong et al. 2023). In addition, urbanization leads to habitat fragmentation and increases the amount of impervious surfaces, which in turn, alters stormwater runoff patterns and ultimately hydrologic systems (Jacobson 2011). Climate change and human activities in concert modify rivers’ hydrological and physicochemical properties (Chen et al. 2017; Gilvear et al. 2002; Xiong et al. 2022), which affects aquatic biota both directly and indirectly (Berger et al. 2017; Xiong et al. 2021). Overall, large river ecosystems face enormous challenges in terms of the sustainable development of water resources and biodiversity conservation (Vörösmarty et al. 2010; Chen et al. 2020; Liu et al. 2023). However, research on riverine ecosystems is relatively limited compared to other aquatic ecosystems such as streams and lakes (Qu et al. 2022; Xiong et al. 2023).
Phytoplankton are primary producers of aquatic ecosystems which perform a vital role in energy flow, material cycling, and material transfer (Song et al. 2020; Zhao et al. 2020). In addition, phytoplankton are sufficiently sensitive so that they serve as important biological indicators reflecting the health of aquatic ecosystems. For example, their densities, biomasses, and species compositions respond rapidly to biological, physical, and chemical changes in their environments (Guo et al. 2019; Shoener et al. 2019; Graco-Roza et al. 2021). With the intensifying impacts of human activities on the environment and the increasing requirements of ecosystem management, ecologists have focused on exploring the effects of water quality, hydrology, climate change, and land use on phytoplankton community—their structure in particular (Monchamp et al. 2018; Zhang et al. 2018a; Fu et al. 2022; Qu et al. 2022). Previous studies have shown that water quality parameters (e.g., nutrients and water temperature, etc.) are the main drivers of phytoplankton community successional processes (Bowes et al. 2012; Song et al. 2022), though these parameters could be modified by watershed land use and climate change (Wu et al. 2023a). Physical characteristics (e.g., river morphology) and light conditions can also affect spatiotemporal distribution of phytoplankton in large rivers (Thorp and Delong 1994; Ding et al. 2022a). High-speed water flows and short residence times influence phytoplankton growth and reproduction (Reynolds 2000). Light is the primary energy source for photosynthesis in phytoplankton, and characteristics such as the ability to achieve net photosynthesis under poor light conditions and high growth rates are necessary for their survival and growth (Reynolds 1994). In addition, habitat and water quality degradation caused by land use changes can broadly affect phytoplankton community composition and resource use efficiency (Kakouei et al. 2021; Peng et al. 2021; Yang et al. 2022).
In addition to the taxonomic aspect, functional approaches have been used to interpret and predict the responses of phytoplankton to environmental changes (Kruk et al. 2002; Reynolds et al. 2002; Padisák et al. 2009). The grouping of phytoplankton with similar or identical physiology, morphology, and survival strategies into a functional group (Reynolds et al. 2002; Padisák et al. 2009) serves as the basic unit of phytoplankton responses to environmental changes, which can reflect their specific habitat requirements (Reynolds et al. 2002). The phytoplankton functional groups approach can supplement and possibly unravel phytoplankton dynamics in ecosystems (Kruk et al. 2002; Padisák et al. 2006; Izaguirre et al. 2012; Sun and Wang 2021). Studies on phytoplankton communities from a functional perspective can add more information to the mechanism of phytoplankton responses to environmental changes (Haque et al. 2021), which have been applied in many aquatic ecosystems (Padisák et al. 2006; Zhang et al. 2007; Abonyi et al. 2012; Kim et al. 2020; Sun and Wang 2021). However, there is relatively less research on phytoplankton functional groups in large rivers (Wang et al. 2021; Ding et al. 2022b).
A combination of multiple driving factors can directly or indirectly alter the phytoplankton community structure (Escalas et al. 2019; Peng et al. 2021; Qu et al. 2022). Consideration of only single environmental factors on phytoplankton community structure cannot effectively detect the complex relationships between phytoplankton and environmental factors, which calls for the joint effects from multiple factors (Peng et al. 2021). Understanding the major environmental factors that regulate riverine phytoplankton communities under multiple stressors is of critical importance, which serves as an essential prerequisite for effective biological assessments as well as riverine aquatic ecosystems management (Song et al. 2020). However, uncertainty exists in production and distribution of phytoplankton in rivers (Qu et al. 2018), and the extent to which land use change affects riverine phytoplankton remains largely unknown (Qu et al. 2022; Yang et al. 2022). In addition, the joint and individual effects of multiple stressors on riverine phytoplankton species and functional groups remain understudied and poorly understood (Qu et al. 2018, 2019).
As the largest river in China, the Yangtze River and its basin supports one-third of the country’s population and contributes about 40% of the gross domestic product (GDP) (Chen et al. 2017). In the past several decades, the Yangtze River has experienced disturbances from climate change and multiple human stressors such as dams, habitat modification, land use change, shipping, sand dredging, and overfishing, which resulted in the destruction of the river’s original natural environment and depletion of aquatic biological resources (Chen et al. 2017, 2020). Climate change and multiple human stressors were found to affect water quality and fish communities in the Yangtze River (Xiong et al. 2021, 2022, 2023), but the response of phytoplankton to these stressors has never been quantified. Previous studies have investigated the biogeographic distribution of phytoplanktonic and benthic diatoms (Wang et al. 2019), the effect of a single environmental metric (e.g., water quality) on phytoplankton community structure (Liu et al. 2019), and using phytoplankton functional groups to evaluate water quality in the Yangtze River (Wu et al. 2023b). Spatially, local river segments and estuaries have received the majority of attentions (Li et al. 2012; Zhu et al. 2013a; Jiang et al. 2014, 2015; Liu et al. 2017; Tan et al. 2017), while the middle and lower reaches of the Yangtze River as a whole, where the most intensive human activity located, are much less studied.
Thus, the objectives of the current study were (1) to analyze community composition and succession of phytoplankton using both taxonomic (i.e., structural) and functional groups and (2) to quantify the effects of multiple driving factors (i.e., climate, habitat, land use, and water quality) on phytoplankton patterns in the middle and lower reaches of the Yangtze River, Assessments were conducted during both wet and dry seasons in 2017–2018. Based on previous studies, we hypothesized that phytoplankton taxa and functional groups would exhibit distinct seasonal patterns, and that water quality will play a critical role in influencing both the taxonomic (structural) and functional composition of phytoplankton communities in the Yangtze River (Xiong et al. 2022, 2023), with certain species are highly abundant in sites with more nutrients and lower turbidity.
Materials and methods
Study area and sampling sites
The Yangtze River is the third-longest in the world and the longest in Asia, which flows through 11 provincial administrative districts in central China, has a total length of around 6300 km (Chen et al. 2017). The Yangtze River basin contains a dense network of rivers and well-developed aquatic systems, including more than 3000 tributaries and 4000 lakes; the basin area accounts for about one-fifth of the total land area of China (Chen et al. 2016). The middle and lower reaches of the Yangtze River are deep and wide enough to allow passage of large commercial ships year round, making it a renowned “golden waterway.” However, along with rapid economic development in this portion of the basin, various human activities have inevitably affected the environmental and ecological conditions, especially urbanization, agriculture, industry, mining, shipping, overfishing, and habitat modifications (Chen et al. 2017, 2020; Xiong et al. 2021, 2022). The middle and lower reaches of the Yangtze River have a subtropical monsoon climate with four distinct seasons, and maximum temperatures reaching 40 °C in summer and minimum temperatures of about − 4 °C in winter. During the wet (July–August) and dry (October–December) seasons of 2017–2018, a total of 30 and 25 river segments were investigated in the middle and lower reaches of the Yangtze River, respectively (Fig. 1, Additional file 1: Table S1). In each of these river segments, a total of three sampling sites per segment were used to collect biological and water quality samples (Xiong et al. 2021, 2022).
Phytoplankton and water quality analysis
At each sampling site, 1-L water samples were taken as quantitative phytoplankton samples. Each sample had 15 mL of Lugol’s solution added and was returned to the laboratory to settle for more than 48 h. Samples were concentrated to 30–50 mL and counted in a 0.1 mL plankton counting chamber (20 × 20 mm) under an optical microscope (BX43, Olympus Corporation, Tokyo) at 400 × magnification (Zhang et al. 2018b; Gao et al. 2023). Phytoplankton were identified at the lowest taxonomic level possible (Hu and Wei 2006), with biomass calculated based on cell volume and density (Huang et al. 2021; Gao et al. 2023). The MacNaughton dominance index (Y) was used to determine phytoplankton dominant species, and species with Y ≥ 0.02 were considered as the dominant species (Han et al. 2020; Hu et al. 2022). The classification of phytoplankton functional groups was based on the criteria proposed by Reynolds et al. (2002) and Padisák et al. (2009). The phytoplankton functional group biomass was the sum of biomass of all phytoplankton species within each functional group. An important or dominant functional group was determined when its relative biomass was 10% or more at a given river segment (Zhu et al. 2013b). The dominant functional group represented the ecological condition and aquatic environment of the region.
A total of 14 water quality parameters were analyzed. In the field, we used a portable multi-parameter water quality meter (YSI ProPlus, Yellow Springs, Ohio USA) to measure dissolved oxygen (DO, mg/L), conductivity (CON, μS/cm), water temperature (WT, °C), and pH. We used a portable turbidimeter (HACH 2100Q; Loveland, Colorado USA) to measure turbidity (TUR, NTU). We then collected 2-L water samples at 0.5 m below the water surface with a 5-L plexiglass sampler at each of the sampling sites, which were immediately transported back to the laboratory with ice for subsequent analysis. In the laboratory, we used standard methods (APHA 2005) to measure total nitrogen (TN, mg/L), total phosphorus (TP, mg/L), orthophosphate (PO4-P, mg/L), nitrate (NO3-N, mg/L), nitrite (NO2-N, mg/L), total ammonia (TAN, mg/L), chemical oxygen demand (COD, mg/L), total suspended solids (TSS, mg/L), and chlorophyll a (Chl-a, μg/L), with details of the analysis methods referenced to previous related studies (Qu et al. 2020; Xiong et al. 2022).
Climate, habitat, and land use data collection
We collected average monthly average air temperature and precipitation for climate data. These data were obtained from the yearbooks of the provinces where the river sections were sampled and the National Environmental Information Center (https://www.ncei.noaa.gov/) (Xiong et al. 2022).
The habitat dataset compiled included hydrologic data (i.e., monthly mean flow and water level) and habitat assessment data (i.e., river bank, river channel, and river bed conditions). Hydrological data were collected from eight national hydrographic stations (i.e., Yichang, Zhicheng, Shashi, Jianli, Luoshan, Hankou, Jiujiang, and Datong) on the Yangtze River main-stem, with data from the nearest hydrographic station used for each sampling site (Xiong et al. 2022).
The river habitat assessment focused on three categories: river bank, river channel, and river bed, with habitat assessment methods and results obtained from a previous study (Lu 2020). The river bank category contained five indicators, including riparian zone width, riparian land use, riparian vegetation condition, river bank modifications, and river bank human activities (Lu 2020). The river channel category included three indicators, namely hydrology and water quality condition, river channel modifications, and river channel habitat complexity (e.g., conditions of islands, sandbars, and secondary channels) (Lu 2020). The river bed category contained two indicators, including river bed substrates, and river bed stability (Lu 2020). Each of these 10 habitat indicators was scored on a 0–20 scale, which corresponded to four evaluation levels ranging from high to low, i.e., excellent (16–20), good (11–15), moderate (6–10), and poor (0–5). The overall habitat score in each site was the sum of the scores from the 10 indicators, which ranged from 0 to 200 (Lu 2020). The total habitat score was also classified into four evaluation levels ranging from high to low, i.e., excellent (150–200), good (120–150), moderate (90–120), and poor (0–90) (Lu 2020).
The riparian land use (e.g., cropland, forest, wetland, and urban) was analyzed using free 10 m resolution images of global land cover form 2017 (FROM-GLC10-2017 V0.1.3) (Gong et al. 2019; Xiong et al. 2021). In the current study, we used the buffer utility in ArcMap 10.7 software (ESRI, USA) to create 5-km × 5-km buffer zones on both banks of the river segments (Xiong et al. 2021).
Data analysis
One-way analysis of variance (ANOVA) was used to test seasonal differences in phytoplankton total abundance, total biomass, species richness, and species diversity (i.e., Shannon–Wiener index [H′], based on species abundances). All data were lg(x + 1) transformed to satisfy normal distribution assumptions (Qu et al. 2020), with all analyses done using IBM SPSS Ver. 24 software. Non-metric multidimensional scaling (NMDS) based on a Bray–Curtis similarity distance measure were used to assess seasonal variations in phytoplankton species abundance and functional group abundance. The analysis of similarity (ANOSIM) was further used to evaluate seasonal differences in communities as depicted by phytoplankton species abundance and functional group abundance. Larger absolute values of R (ANOSIM Global R) indicated greater relative differences, whereas smaller p values indicated more significant differences. Analysis and visualization of NMDS and ANOSIM were done using the ‘vegan’ and ‘ggplot2’ packages in R. The similarity percentage (SIMPER) was used to identify the major taxa responsible for the seasonal differences in the communities depicted by both phytoplankton species abundance and functional group biomass (Yang et al. 2022) in R.
Mantel tests are correlation test methods for determining the correlation between two sets of distance measure matrices. We used mantel tests to determine relationships between phytoplankton indices (i.e., total abundance, total biomass, species richness, and species diversity) and environmental variables (i.e., climate, habitat, land use, and water quality) (Rusanov et al. 2022). Phytoplankton and environmental variables were used with the Bray–Curtis distance and Euclidean distance, respectively. The environmental data were standardized to eliminate dimensional differences, using the ‘vegan’ package in R for the analysis. To quantify joint and separate effects of climate, habitat, land use, and water quality on phytoplankton taxonomic and functional group, a variance partitioning analysis (VPA) was performed using the ‘varpart’ function in the ‘vegan’ package. Taxonomic and functional groups with < 10% frequency of occurrence were removed before analysis. Environmental variables were used as the explanatory variables, and phytoplankton species abundance and functional group abundance were used as the response variables. Hellinger transformations were applied to the response variables to reduce the impacts of rare species and double-zero problems (Qu et al. 2023), and log10 (x + 1) transformations were applied to the explanatory variables. The forward selection was performed using the “ordiR2step” function in the ‘vegan’ package to screen explanatory variables based on 999 permutation tests, and each response variable (taxonomic and functional groups) was screened separately (Rusanov et al. 2022). The overall variance was divided into components based on adjusted R values: i.e., climate, habitat, land use, water quality, and residuals. ANOVA was used to test the significance of each category of these environmental variables. The significance level for all statistical analyses was set at 0.05.
Results
Characteristics of phytoplankton taxonomic structure
A total of 150 taxa from 8 phyla of phytoplankton were identified from the middle and lower reaches of the Yangtze River. Chlorophyta had the greatest species richness (67 species), followed by bacillariophyta (49 species), cyanophyta (21 species), dinophyta (6 species), and euglenophyta (4 species), with cryptophyta, chrysophyta, and xanthophyta having the lowest species richness (1 species each). Overall, there were 140 and 84 phytoplankton taxa identified during the wet and dry seasons, respectively; mean values for each season were 18 and 16 species, respectively (Fig. 2c). Phytoplankton total abundance in the wet and dry seasons were 1.53 × 104–1.04 × 106 cells/L and 1.62 × 104–1.05 × 106 cells/L, respectively; mean values for each season were 3.43 × 105 cells/L and 1.81 × 105 cell/L, respectively (Fig. 2a). Similarly, Phytoplankton total biomass during the wet and dry seasons were 0.02–1.01 mg/L and 0.01–0.39 mg/L, respectively, with mean values of 0.16 mg/L and 0.10 mg/L, respectively (Fig. 2b). Phytoplankton diversity (H′) ranged from 0.17 to 2.64 and 0.53 to 2.55 during the wet and dry seasons, respectively (Fig. 2d). ANOVA indicated that phytoplankton total abundances, total biomasses, and species richness were significantly greater (P < 0.01) during the wet season compared to the dry season (Fig. 2). Phytoplankton diversity was similar between seasons.
Cyanophyta, bacillariophyta, and chlorophyta were the dominant phyla in terms of phytoplankton abundance, with average relative abundances of 55%, 28%, and 16%, respectively. The relative abundance of cyanophyta decreased in the dry season compared to the wet season, while the relative abundance of bacillariophyta and chlorophyta exhibited opposite trends. Biomass of bacillariophyta was the greatest throughout the year (58%), followed by chlorophyta (22%), dinophyta (13%) and cyanophyta (4%). Biomass of bacillariophyta increased in the dry season compared to the wet season. In terms of abundance, four and seven phytoplankton species were dominant in the wet and dry seasons, respectively, with Pseudanabaena sp., Oscillatoria sp., Cyclotella sp., and Melosira granulata being dominant in both seasons. In addition, Scenedesmus sp., Synedra sp., and Melosira granulata var. angustissima also were the dominant phytoplankton species in the dry season. NMDS and ANOSIM indicated significant differences in phytoplankton community composition between the wet and dry seasons (Global R = 0.20, P < 0.01) (Fig. 3a), with cyanobacteria, bacillariophyta, and chlorophyta contributing to 99% of the seasonal variation at the phylum level (Fig. 3b). Oscillatoria sp., Pseudanabaena sp., and Melosira granulata contributed to 15%, 12%, and 8% of the seasonal variation at the species level, respectively (Fig. 3c, Additional file 1: Table S2).
Characteristics of phytoplankton functional groups
Phytoplankton in the middle and lower reaches of the Yangtze River were classified into 26 functional groups, including A, B, C, D, E, F, G, H1, J, Lo, M, MP, N, NA, P, S1, S2, SN, T, TB, W1, W2, X1, X2, X3, and Y (Additional file 1: Table S3). In the wet season, there were 26 functional groups, 12 of which (i.e., A, B, D, F, H1, J, Lo, MP, P, TB, W2, and X3) were dominant. Functional groups P, J, and Lo had the greatest mean relative biomasses at 38%, 17%, and 13%, respectively (Fig. 4a). In the dry season, there were 23 functional groups, 10 of which (i.e., B, D, J, Lo, MP, P, TB, W1, X3, and Y) were dominant. The mean relative biomass of functional groups P, J, and Lo decreased to 35%, 13%, and 10%, respectively (Fig. 4b). NMDS and ANOSIM indicated a significant difference (P < 0.01) in the composition of phytoplankton functional groups between the wet and dry seasons (Fig. 5a), with functional groups P, Lo, and J contributing the most seasonal variation at with 17%, 9%, and 9%, respectively (Fig. 5b, Additional file 1: Table S4).
Driving factors of phytoplankton community
Mantel tests detected significant effects of environmental factors on phytoplankton total abundances, total biomasses, and species richness (P < 0.05). Water quality had significant effects on phytoplankton abundances, biomasses, species richness, and diversity (P < 0.05). Climate had significant effects on phytoplankton abundances and species richness (P < 0.01) whereas habitat significantly affected only abundances (P < 0.05) (Additional file 1: Table S5).
The variance partitioning indicated that climate, habitat, land use, and water quality all made contributions to shaping the structure of phytoplankton communities, both taxonomically and functionally (Fig. 6). The total variance explained by environmental factors for phytoplankton taxonomic and functional groups was 24% and 20%, respectively. Water quality (mainly NO3-N, TAN, TSS, and TUR) and habitat (mainly water flow, water level, and river channel and bank conditions) explained most of the variation in phytoplankton species abundances (water quality total 17%, independently 10%; habitat total 10%, independently 5%) and functional groups biomasses (water quality total 12%, independently 5%; habitat total 11%, independently 4%) (Fig. 6, Additional file 1: Table S6). Climate (mainly air temperature and precipitation) explained some of the variation in phytoplankton species abundances (total 9%, independently2%) and functional groups biomasses (total 8%, independently 2%) (Fig. 6, Additional file 1: Table S6). Land use (mainly wetland and forest) explained the least variance in phytoplankton communities whether expressed taxonomically and functionally (Fig. 6, Additional file 1: Table S6).
Discussion
Phytoplankton community structure
We identified a total of 150 taxa of phytoplankton in the middle and lower reaches of the Yangtze River, more than the 117 species found by Wu et al. (2023b) and the 59 species found by Liu et al. (2019). This could be related to larger sampling efforts (e.g., spatial ranges, number of sites, multiple seasons, and multiple years) in the current study. Cyanobacteria, bacillariophyta, and chlorophyta were the main contributors to phytoplankton abundance, which was consistent with previous findings (Liu et al. 2019). High nitrogen and low phosphorus concentrations are beneficial to preventing cyanobacterial blooms (Wang et al. 2020). When the ratio of TN/TP is greater than 22, it is phosphorus-limited and not conducive to cyanobacterial growth and reproduction (Ding et al. 2022a). In the current study, the wet and dry season average TN/TP ratios were 18.6 and 21.7 in the Yangtze River, respectively, which would be consistent with a dominance of cyanobacteria. We found that bacillariophyta were dominant in terms of biomass, which occur frequently in other rivers (Ding et al. 2022a; Rusanov et al. 2022). We detected greater phytoplankton abundances, biomasses, and species richness during the wet season. Sufficient light and greater temperatures during the wet season (i.e., summer) appeared to have facilitated the growth and reproduction of phytoplankton (Tian et al. 2021). The relative abundances of cyanobacteria and chlorophyta also were greater during the wet season than the dry season, while diatoms exhibited the opposite pattern. Water temperature affects growth of phytoplankton by controlling the enzymatic reactions of photosynthesis and the intensity of respiration, which consequently affects variations in community structure (Blinn 1993). Cyanobacteria and chlorophyta prefer warmer conditions, while diatoms can be dominant at a wide range of water temperatures. In addition, the runoff from abundant rainfall during the wet season increases nutrient inputs to the river (Xiong et al. 2022), which could have favored the growth and reproduction of filamentous cyanobacteria (Duong et al. 2019). The dominant species Pseudanabaena sp. and Oscillatoria sp. are filamentous cyanobacteria with high metabolic capacities and good mobility, which would certainly be a favorable trait for survival and reproduction in the Yangtze River, where flows and water level fluctuations can be excessive (Wang et al. 2020).
Phytoplankton functional group MP (including Oscillatoria sp., Navicula sp., Surirella sp., Cymbella sp., etc.) are able to adapt to turbid environments with frequent disturbances (Padisák et al. 2009). Functional groups B (Cyclotella sp.) and D (including Synedra sp. and Nitzschia sp.) that are mainly composed of diatoms, have larger sizes, and higher surface areas, which facilitates efficient light harvesting and is advantageous for growth and reproduction in highly turbid conditions (Padisák et al. 2009). The Yangtze River is the largest river in China with high flow velocities and high sand content. It seems plausible that these conditions coupled with the turbid water environment would facilitate MP, and B and D at becoming the dominant functional groups. Functional groups P, J, and Lo generally explained the most seasonal community variation and all had lower relative biomass in the dry season than those in the wet season. Functional group J (including Pediastrum sp., Tetraedron sp., Crucigenia sp., Scenedesmus sp., and Coelastrum sp.) was mainly composed of chlorophyta whereas functional group P (including Closterium sp., Melosira sp.) consisted mainly of chlorophyta and diatoms. These functional groups are adapted to frequently stirred waters with high nutrient levels (Padisák et al. 2009). The functional group Lo (including Merismopedia sp., Peridinium sp., Ceratium sp., and Gymnodinium sp.) is one of the most common taxa in large rivers. They are adapted to a wide variety of habitats, including both deep and shallow water, and occur across a wide range of nutrient concentrations (Padisák et al. 2009; Xiao et al. 2011). Peridinium sp., the key species of functional group Lo, prefers to reproduce at high temperatures (20–27 °C) (Grigorszky et al. 2006), while the high-temperature environment in the Yangtze River during the wet season also appears suitable for functional groups P and J (Liu et al. 2019). Urban and cropland are the main land uses in the middle and lower reaches of the Yangtze River (Xiong et al. 2021). Abundant rainfall during the wet season transfers pollutants from land into the river through surface runoff processes, which would be consistent with nutrient concentration increases in the river (Tong et al. 2017). Previous studies in the Yangtze River indicated that COD, turbidity, TP, and TN concentrations also were significantly greater during wet than dry seasons (Xiong et al. 2022), which also would be favorable for functional groups P, J, and Lo (Padisák et al. 2009; Long et al. 2020).
Driving factors of phytoplankton communities
Phytoplankton community structure in aquatic ecosystems is determined by the dynamic interplay of growth and losses. To a large extent, growth is regulated by resource availability, i.e., nutrients, light, temperature, etc., while losses are influenced by scouring, grazing, cell death, and subsidence (Interlandi and Kilham 2001; Reynolds 2006; Cao et al. 2018; Sommer et al. 2018). Environmental variables can disrupt the ecological balance of more pristine waters, thus, leading to a new community equilibrium through successional processes. Multiple factors can affect phytoplankton communities, including water quality, climate, hydrology, and land use among others. However, the influences that drive phytoplankton succession often vary among study areas (Xu et al. 2010; Zhou et al. 2016; Wu et al. 2019; Huang et al. 2021; Qu et al. 2022; Rusanov et al. 2022). Water quality and physical habitat influence riverine aquatic life significantly (Pilière et al. 2014; Green et al. 2022), and local factors such as water quality and hydrology have direct impacts on riverine phytoplankton (Tian et al. 2021). Our results demonstrate that water quality, habitat, climate, and land use all played important roles in shaping phytoplankton taxonomic and functional groups, with water quality and habitat being the main driving factors.
Water quality parameters such as nitrogenous nutrients, turbidity, TSS, and WT were important in affecting the variations in phytoplankton taxonomic and functional groups. This finding was consistent with previous studies (Michalak 2016; Tian et al. 2021) where nutrient levels were extremely critical for phytoplankton growth and community succession (Baines and Pace 1991; Xu et al. 2010; Liu et al. 2019). Although nitrogenous nutrients are often the main factors driving phytoplankton functional groups (Ding et al. 2022b), most phytoplankton prioritize the utilization of NH4-N (Dortch 1990), with combinations of high nitrogen and low phosphorus beneficial at preventing cyanobacterial blooms (Wang et al. 2020). The Yangtze River is a typical river with poor nutrients (Liu et al. 2019), and nitrogen is critical for phytoplankton growth when it is in an oligotrophic condition (Jiang and Nakano 2022). In contrast, low nitrogen has less effect on nitrogen-fixing cyanobacteria (Boyer et al. 2006), thus, it is likely that Oscillatoria sp. had been dominant for a long period of time before the study. The underwater light condition is a major factor affecting phytoplankton growth, which had been detected in lake studies in the Yangtze River basin (Wu et al. 2013, 2019). Yang et al. (2019) also reported that mixing regime, water temperature, and light availability were the driving factors controlling phytoplankton community dynamics in an estuarine reservoir of the Yangtze River. Since discharge, turbidity, and suspended solids are the main environmental factors affecting river phytoplankton, they are able to perform their functions by regulating water quantity and underwater light (Salmaso and Braioni 2008; Jia et al. 2020; Tian et al. 2021; Ding et al. 2022b). As a large river with high water volume and high sand content, the species selectivity in turbid habitats of the Yangtze River may favor diatoms, especially those with high surface/volume ratios and low light acclimation (Reynolds 1994). In addition to water quality, flow, and channel habitat conditions, river bank conditions such as riparian buffers can be critical in that they protect the river from negative impacts from surrounding land uses (Mundahl and Mundahl 2022). Adequate areas and intact vegetated buffers can effectively intercept surface runoff into streams. Vegetation can filter and absorb pollutants to reduce nutrient levels in surface runoff and groundwater (Osborne and Kovacic 1993; Yates et al. 2007; Mundahl and Mundahl 2022) by reducing nutrients in the river, thus, increasing water transparency and driving phytoplankton succession.
Climate also plays an important role in driving variations in phytoplankton taxonomic and functional groups. Precipitation and air temperature contributed significantly to phytoplankton community changes, which have been reported in other studies (Blois et al. 2013). Climate is one of the major factors contributing to differences across rivers worldwide. Thus, it is plausible that phytoplankton variation in large rivers may be largely driven by interregional differences in climate (Rusanov et al. 2022). Ding et al. (2022b) found significant effects of precipitation and air temperature on phytoplankton diversity in spring and autumn in the Yellow River, China. Greater air temperatures also can lead to significant increases in river phytoplankton, especially cyanobacteria (Haakonsson et al. 2017; Bao et al. 2022). Conversely, precipitation effects on phytoplankton community structure are related to altered nutrients and other abiotic factors in aquatic ecosystems (Zhou et al. 2016; Peng et al. 2021).
Land use contributed less to the variations in phytoplankton taxomonic and functional groups than other factors in the current study. The effects of land use on phytoplankton are mainly through the increase or decrease of nutrients and water transparency to regulate the phytoplankton community, with the effects differing across different between land uses (Peng et al. 2021). Moreover, land use is a comprehensive indicator that combines multiple factors (Qu et al. 2022), and is associated with nutrients (Xiong et al. 2022), hydrology (Guse et al. 2015), physical habitat (Mundahl and Mundahl 2022), and other pollutants (Kelso and Baker 2022). However, land use in the Yangtze River seems to affect other aquatic biota such as fish more directly than other organisms (Xiong et al. 2021, 2023). This may jointly result from the water quality and habitat characteristics in large rivers (e.g., high flows, turbid water, rapid changes in habitat conditions) and the succession of phytoplankton in these systems (Reynolds 2006; Padisák et al. 2006). The different responses of aquatic biota to land use and other human stressors warrants further comprehensive studies in large river ecosystems.
Limitations and recommendations
Limitations exist in the current study. First, we focused on seasonal variations of phytoplankton during the 2 year period of wet and dry seasons. However, results from this relatively low-frequency, short-timespan monitoring may not fully represent the overall pattern of phytoplankton community dynamics in the Yangtze River. In the future, studies involving more seasons and larger spatial scales would be recommended. Second, the Yangtze River, as China’s largest river, is subject to a complex and diverse array of anthropogenic disturbances (Chen et al. 2016; Chen et al. 2017, 2020), including dams, navigation, sand mining, overfishing, urbanization, and riparian development among others—all of which have been demonstrating as having significant effects on river ecosystems (Zhang et al. 2019; Xiong et al. 2021, 2022, 2023; Gao et al. 2023). In this study, we focused on the effects of land use, water quality, habitat, and climate on phytoplankton communities, However, there are still a multitude of other unmeasured or unquantified factors that drive variations of phytoplankton communities (Sommer et al. 2012). Phytoplankton is an intermediate link in the energy flow of aquatic ecosystems, which should not only take into account the effects of numerous abiotic factors (Lv et al. 2014), but also consider the effects of biotic factors, such as predation by zooplankton and planktivorous fishes (Zhang et al. 2018b; Yin et al. 2022; Guo et al. 2023). Interspecific interactions such as these were not considered in this study, though they also may play important roles in shaping phytoplankton community patterns (Yang et al. 2018). In summary, longer-term, more intensive, and studies across larger spatial scales are needed in the future to fully understand phytoplankton patterns and their driving mechanisms in large river systems such as the Yangtze River.
Conclusions
The main conclusions of this study include:
-
(1)
A total of 150 phytoplankton taxa belonging to 26 functional groups were identified in the middle and lower reaches of the Yangtze River. The phytoplankton were mainly composed of cyanobacteria, bacillariophyta, and chlorophyta, and the abundance, biomass, and species richness of phytoplankton were significantly greater during the wet season compared to the dry season.
-
(2)
Phytoplankton taxonomic and functional group compositions differed significantly between seasons. The taxonomic variation in phytoplankton communities between wet and dry seasons were driven mainly by the abundances of Oscillatoria sp., Pseudanabaena sp., and Melosira granulate. The variation in the functional composition of phytoplankton communities between seasons was mainly contributed by P, Lo, and J.
-
(3)
Water quality (NO3-N, total suspended solids, turbidity) and habitat (water flow, riverbank and river channel conditions) were the primary driving factors for phytoplankton community variation, followed by climate (air temperature and precipitation) and land use.
Availability of data and materials
All data will be available in the data center of Institute of Hydrobiology, Chinese Academy of Sciences (www.ihb.ac.cn).
References
Abonyi A, Leitão M, Lançon AM, Padisák J (2012) Phytoplankton functional groups as indicators of human impacts along the River Loire (France). Hydrobiologia 698:233–249. https://doi.org/10.1007/s10750-012-1130-0
APHA (American Public Health Association) (2005) Standard methods for the examination of water and wastewater, 21st ed. American Public Health Association, American Water Works Association, Water Environment Federation, Washington, DC, USA
Arneth A, Shin Y-J, Leadley P, Rondinini C, Bukvareva E, Kolb M, Midgley GF, Oberdorff T, Palomo I, Saito O (2020) Post-2020 biodiversity targets need to embrace climate change. PNAS 117:30882–30891. https://doi.org/10.1073/pnas.2009584117
Baines SB, Pace ML (1991) The production of dissolved organic matter by phytoplankton and its importance to bacteria: patterns across marine and freshwater systems. Limnol Oceanogr 36:1078–1090. https://doi.org/10.4319/lo.1991.36.6.1078
Bao L, Chen J, Tong H, Qian J, Li X (2022) Phytoplankton dynamics and implications for eutrophication management in an urban river with a series of rubber dams. J Environ Manage 311:114865. https://doi.org/10.1016/j.jenvman.2022.114865
Berger E, Haase P, Kuemmerlen M, Leps M, Schäfer RB, Sundermann A (2017) Water quality variables and pollution sources shaping stream macroinvertebrate communities. Sci Total Environ 587–588:1–10. https://doi.org/10.1016/j.scitotenv.2017.02.031
Best J (2019) Anthropogenic stresses on the world’s big rivers. Nat Geosci 12:7–21. https://doi.org/10.1038/s41561-018-0262-x
Birk S (2019) Detecting and quantifying the impact of multiple stress on river ecosystems. In: Sabater S, Elosegi A, Ludwig R (eds) Multiple stressors in river ecosystems, vol 14. Elsevier, Amsterdam, pp 235–253
Blinn DW (1993) Diatom community structure along physicochemical gradients in saline lakes. Ecology 74:1246–1263. https://doi.org/10.2307/1940494
Blois JL, Williams JW, Fitzpatrick MC, Jackson ST, Ferrier S (2013) Space can substitute for time in predicting climate-change effects on biodiversity. PNAS 110:9374–9379. https://doi.org/10.1073/pnas.1220228110
Bowes MJ, Gozzard E, Johnson AC, Scarlett PM, Roberts C, Read DS, Armstrong LK, Harman SA, Wickham HD (2012) Spatial and temporal changes in chlorophyll-a concentrations in the River Thames basin, UK: are phosphorus concentrations beginning to limit phytoplankton biomass? Sci Total Environ 426:45–55. https://doi.org/10.1016/j.scitotenv.2012.02.056
Boyer JN, Dailey SK, Gibson PJ, Rogers MT, Mir-Gonzalez D (2006) The role of dissolved organic matter bioavailability in promoting phytoplankton blooms in Florida Bay. Hydrobiologia 569:71–85. https://doi.org/10.1007/s10750-006-0123-2
Cao J, Hou Z, Li Z, Chu Z, Yang P, Zheng B (2018) Succession of phytoplankton functional groups and their driving factors in a subtropical plateau lake. Sci Total Environ 631–632:1127–1137. https://doi.org/10.1016/j.scitotenv.2018.03.026
Chen Y, Chapman DC, Jackson JR, Chen D, Li Z, Kilgore KJ, Phelps Q, Eggleton MA (eds) (2016) Fishery resources, environment, and conservation in the Mississippi and Yangtze (Changjiang) River Basins, vol 84. American Fisheries Society, Bethesda
Chen Y, Zhang S, Huang D, Li B, Liu J, Liu W, Ma J, Wang F, Wang Y, Wu S, Wu Y, Yan J, Guo C, Xin W, Wang H (2017) The development of China’s Yangtze River Economic Belt: how to make it in a green way? Sci Bull 62:648–651. https://doi.org/10.1016/j.scib.2017.04.009
Chen Y, Qu X, Xiong F, Lu Y, Wang L, Hughes RM (2020) Challenges to saving China’s freshwater biodiversity: fishery exploitation and landscape pressures. Ambio 49:926–938. https://doi.org/10.1007/s13280-019-01246-2
Ding Y, Li M, Pan B, Zhao G, Gao L (2022a) Disentangling the drivers of phytoplankton community composition in a heavily sediment-laden transcontinental river. J Environ Manage 302:113939. https://doi.org/10.1016/j.jenvman.2021.113939
Ding Y, Pan B, Zhao X, Zhao G, Han X, Li M (2022b) Will a heavy sediment load affect responses of phytoplankton functional groups to aquatic environmental changes in different water body types? Sci Total Environ 837:155863. https://doi.org/10.1016/j.scitotenv.2022.155863
Dortch Q (1990) The interaction between ammonium and nitrate uptake in phytoplankton. Mar Ecol Prog Ser 61:183–201. https://doi.org/10.3354/meps061183
Dudgeon D, Arthington AH, Gessner MO, Kawabata Z-I, Knowler DJ, Lévêque C, Naiman RJ, Prieur-Richard A-H, Soto D, Stiassny MLJ, Sullivan CA (2006) Freshwater biodiversity: importance, threats, status and conservation challenges. Biol Rev 81:163–182. https://doi.org/10.1017/S1464793105006950
Duong TT, Hoang TTH, Nguyen TK, Le TPQ, Le ND, Dang DK, Lu X, Bui MH, Trinh QH, Dinh THV, Pham TD, Rochelle-Newall E (2019) Factors structuring phytoplankton community in a large tropical river: case study in the Red River (Vietnam). Limnologica 76:82–93. https://doi.org/10.1016/j.limno.2019.04.003
Escalas A, Catherine A, Maloufi S, Cellamare M, Hamlaoui S, Yéprémian C, Louvard C, Troussellier M, Bernard C (2019) Drivers and ecological consequences of dominance in periurban phytoplankton communities using networks approaches. Water Res 163:114893. https://doi.org/10.1016/j.watres.2019.114893
Fang Y, Jawitz JW (2019) The evolution of human population distance to water in the USA from 1790 to 2010. Nat Commun 10:1–8. https://doi.org/10.1038/s41467-019-08366-z
Fu H, Chen L, Ge Y, Wu A, Liu H, Li W, Yuan G, Jeppesen E (2022) Linking human activities and global climatic oscillation to phytoplankton dynamics in a subtropical lake. Water Res 208:117866. https://doi.org/10.1016/j.watres.2021.117866
Gao W, Xiong F, Lu Y, Qu X, Xin W, Chen Y (2023) Development of a phytoplankton-based index of biotic integrity for ecological health assessment in the Yangtze River. Ecol Process 12:41. https://doi.org/10.1186/s13717-023-00456-7
Gilvear DJ, Heal KV, Stephen A (2002) Hydrology and the ecological quality of Scottish river ecosystems. Sci Total Environ 294:131–159. https://doi.org/10.1016/S0048-9697(02)00060-8
Gong P, Liu H, Zhang M, Li C, Wang J, Huang H, Clinton N, Ji L, Li W, Bai Y, Chen B, Xu B, Zhu Z, Yuan C, Suen H, Guo J, Xu N, Li W, Zhao Y, Yang J, Yu C, Wang X, Fu H, Yu L, Dronova I, Hui F, Cheng X, Shi X, Xiao F, Liu Q, Song L (2019) Stable classification with limited sample: transferring a 30-m resolution sample set collected in 2015 to mapping 10-m resolution global land cover in 2017. Sci Bull 64:370–373. https://doi.org/10.1016/j.scib.2019.03.002
Graco-Roza C, Soininen J, Corrêa G, Pacheco FS, Miranda M, Domingos P, Marinho MM (2021) Functional rather than taxonomic diversity reveals changes in the phytoplankton community of a large dammed river. Ecol Indic 121:107048. https://doi.org/10.1016/j.ecolind.2020.107048
Green NS, Li S, Maul JD, Overmyer JP (2022) Natural and anthropogenic factors and their interactions drive stream community integrity in a North American river basin at a large spatial scale. Sci Total Environ 835:155344. https://doi.org/10.1016/j.scitotenv.2022.155344
Grigorszky I, Kiss KT, Béres V, Bácsi I, M-Hamvas M, Máthé C, Vasas G, Padisák J, Borics G, Gligora M, Borbély G (2006) The effects of temperature, nitrogen, and phosphorus on the encystment of Peridinium cinctum, Stein (Dinophyta). Hydrobiologia 563:527–535. https://doi.org/10.1007/s10750-006-0037-z
Guo K, Wu N, Wang C, Yang D, He Y, He J, Chai Y, Duan M, Huang X, Riis T (2019) Trait dependent roles of environmental factors, spatial processes and grazing pressure on lake phytoplankton metacommunity. Ecol Indic 103:312–320. https://doi.org/10.1016/j.ecolind.2019.04.028
Guo C, Li S, Ke J, Liao C, Hansen AG, Jeppesen E, Zhang T, Li W, Liu J (2023) The feeding habits of small-bodied fishes mediate the strength of top-down effects on plankton and water quality in shallow subtropical lakes. Water Res 233:119705. https://doi.org/10.1016/j.watres.2023.119705
Guse B, Kail J, Radinger J, Schröder M, Kiesel J, Hering D, Wolter C, Fohrer N (2015) Eco-hydrologic model cascades: Simulating land use and climate change impacts on hydrology, hydraulics and habitats for fish and macroinvertebrates. Sci Total Environ 533:542–556. https://doi.org/10.1016/j.scitotenv.2015.05.078
Haakonsson S, Rodríguez-Gallego L, Somma A, Bonilla S (2017) Temperature and precipitation shape the distribution of harmful cyanobacteria in subtropical lotic and lentic ecosystems. Sci Total Environ 609:1132–1139. https://doi.org/10.1016/j.scitotenv.2017.07.067
Han X, Pan B, Zhao G, Li D, Sun H, Zhu P, Lu Y (2020) Local and geographical factors jointly drive elevational patterns of phytoplankton in the source region of the Yangtze River, China. River Res Applic 37:1145–1155. https://doi.org/10.1002/rra.3711
Haque MA, Jewel MAS, Akhi MM, Atique U, Paul AK, Iqbal S, Islam MS, Das SK, Alam MM (2021) Seasonal dynamics of phytoplankton community and functional groups in a tropical river. Environ Monit Assess 193:1–16. https://doi.org/10.1007/s10661-021-09500-5
Hu H, Wei Y (2006) The freshwater algae of China: systematics, taxonomy and ecology. Beijing Science Press, Beijing
Hu X, Hu M, Zhu Y, Wang G, Xue B, Sangam S (2022) Phytoplankton community variation and ecological health assessment for impounded lakes along the eastern route of China’s South-to-North Water Diversion Project. J Environ Manage 318:115561. https://doi.org/10.1016/j.jenvman.2022.115561
Huang G, Wang X, Chen Y, Deng L, Xu D (2021) Survival strategies of phytoplankton functional groups to environmental factors in a drinking water reservoir, central China. Ann Limnol Int J Lim 57:18. https://doi.org/10.1051/limn/2021016
Interlandi SJ, Kilham SS (2001) Limiting resources and the regulation of diversity in phytoplankton communities. Ecology 82:1270–1282. https://doi.org/10.1890/0012-9658(2001)082[1270:LRATRO]2.0.CO;2
Izaguirre I, Allende L, Escaray R, Bustingorry J, Pérez G, Tell G (2012) Comparison of morpho-functional phytoplankton classifications in human-impacted shallow lakes with different stable states. Hydrobiologia 698:203–216. https://doi.org/10.1007/s10750-012-1069-1
Jacobson CR (2011) Identification and quantification of the hydrological impacts of imperviousness in urban catchments: a review. J Environ Manage 92:1438–1448. https://doi.org/10.1016/j.jenvman.2011.01.018
Jia J, Gao Y, Zhou F, Shi K, Johnes PJ, Dungait JAJ, Ma M, Lu Y (2020) Identifying the main drivers of change of phytoplankton community structure and gross primary productivity in a river-lake system. J Hydrol 583:124633. https://doi.org/10.1016/j.jhydrol.2020.124633
Jiang M, Nakano S-I (2022) The crucial influence of trophic status on the relative requirement of nitrogen to phosphorus for phytoplankton growth. Water Res 222:118868. https://doi.org/10.1016/j.watres.2022.118868
Jiang Z, Liu J, Chen J, Chen Q, Yan X, Xuan J, Zeng J (2014) Responses of summer phytoplankton community to drastic environmental changes in the Changjiang (Yangtze River) estuary during the past 50 years. Water Res 54:1–11. https://doi.org/10.1016/j.watres.2014.01.032
Jiang Z, Chen J, Zhou F, Shou L, Chen Q, Tao B, Yan X, Wang K (2015) Controlling factors of summer phytoplankton community in the Changjiang (Yangtze River) estuary and adjacent East China Sea shelf. Cont Shelf Res 101:71–84. https://doi.org/10.1016/j.csr.2015.04.009
Kakouei K, Kraemer BM, Anneville O, Carvalho L, Feuchtmayr H, Graham JL, Higgins S, Pomati F, Rudstam LG, Stockwell JD, Thackeray SJ, Vanni MJ, Adrian R (2021) Phytoplankton and cyanobacteria abundances in mid-21st century lakes depend strongly on future land use and climate projections. Global Change Biol 27:6409–6422. https://doi.org/10.1111/gcb.15866
Kelso JE, Baker MA (2022) Organic matter sources and composition in four watersheds with mixed land cover. Hydrobiologia 849:2663–2682. https://doi.org/10.1007/s10750-022-04884-y
Kim HG, Hong S, Kim D-K, Joo G-J (2020) Drivers shaping episodic and gradual changes in phytoplankton community succession: taxonomic versus functional groups. Sci Total Environ 734:138940. https://doi.org/10.1016/j.scitotenv.2020.138940
Kruk C, Mazzeo N, Lacerot G, Reynolds CS (2002) Classification schemes for phytoplankton: a local validation of a functional approach to the analysis of species temporal replacement. J Plankton Res 24:901–912. https://doi.org/10.1093/plankt/24.9.901
Li S, Fan Z, Ren Y, Chen D, Liu S, Duan X (2012) Annual characteristics of phytoplankton communities at Yichang to Chenglingji section in the middle reach of the Yangtze River. Resour Environ Yangtze Basin 21:62–68
Liu M, Li P, Zeng Z, Huang C, Liu S (2017) The characteristic of phytoplankton community structure in Anqing section of the Yangtze River. Freshw Fish 47:29–36. https://doi.org/10.3969/j.issn.1000-6907.2017.04.006
Liu Y, Xu X, Wang T, Ni J (2019) Microscopic view of phytoplankton along the Yangtze River. Sci China Technol Sci 62:1873–1884. https://doi.org/10.1007/s11431-019-9545-y
Liu H, Qu X, Xia W, Chen Y (2023) Taxonomic, functional, and phylogenetic diversity patterns reveal different processes shaping river fish assemblages in the Eastern Huai River Basin, China. Water Biol Security 2:100078. https://doi.org/10.1016/j.watbs.2022.100078
Long S, Zhang T, Fan J, Li C, Xiong K (2020) Responses of phytoplankton functional groups to environmental factors in the Pearl River, South China. Environ Sci Pollut Res 27:42242–42253. https://doi.org/10.1007/s11356-020-08344-9
Lu Y (2020) Study of habitat assessment and restoration strategies of the main stem in the middle and lower reaches of Yangtze River. Master Thesis, Dalian Ocean University
Lv H, Yang J, Liu L, Yu X, Yu Z, Chiang P (2014) Temperature and nutrients are significant drivers of seasonal shift in phytoplankton community from a drinking water reservoir, subtropical China. Environ Sci Pollut Res 21:5917–5928. https://doi.org/10.1007/s11356-014-2534-3
Michalak AM (2016) Study role of climate change in extreme threats to water quality. Nature 535:349–350. https://doi.org/10.1038/535349a
Monchamp ME, Spaak P, Domaizon I, Dubois N, Bouffard D, Pomati F (2018) Homogenization of lake cyanobacterial communities over a century of climate change and eutrophication. Nat Ecol Evol 2:317–324. https://doi.org/10.1038/s41559-017-0407-0
Mundahl ND, Mundahl ED (2022) Aquatic community structure and stream habitat in a karst agricultural landscape. Ecol Process 11:18. https://doi.org/10.1186/s13717-022-00365-1
Osborne LL, Kovacic DA (1993) Riparian vegetated buffer strips in water-quality restoration and stream management. Freshw Biol 29:243–258. https://doi.org/10.1111/j.1365-2427.1993.tb00761.x
Padisák J, Borics G, Grigorszky I, Soróczki-Pintér É (2006) Use of phytoplankton assemblages for monitoring ecological status of lakes within the Water Framework Directive: the assemblage index. Hydrobiologia 553:1–14. https://doi.org/10.1007/s10750-005-1393-9
Padisák J, Crossetti LO, Naselli-Flores L (2009) Use and misuse in the application of the phytoplankton functional classification: a critical review with updates. Hydrobiologia 621:1–19. https://doi.org/10.1007/s10750-008-9645-0
Peng X, Zhang L, Li Y, Lin Q, He C, Huang S, Li H, Zhang X, Liu B, Ge F, Zhou Q, Zhang Y, Wu Z (2021) The changing characteristics of phytoplankton community and biomass in subtropical shallow lakes: coupling effects of land use patterns and lake morphology. Water Res 200:117235. https://doi.org/10.1016/j.watres.2021.117235
Pilière A, Schipper AM, Breure AM, Posthuma L, De Zwart D, Dyer SD, Huijbregts MA (2014) Comparing responses of freshwater fish and invertebrate community integrity along multiple environmental gradients. Ecol Indic 43:215–226. https://doi.org/10.1016/j.ecolind.2014.02.019
Qu Y, Wu N, Guse B, Fohrer N (2018) Riverine phytoplankton shifting along a lentic-lotic continuum under hydrological, physiochemical conditions and species dispersal. Sci Total Environ 619–620:1628–1636. https://doi.org/10.1016/j.scitotenv.2017.10.139
Qu Y, Wu N, Guse B, Makarevičiūtė K, Sun X, Fohrer N (2019) Riverine phytoplankton functional groups response to multiple stressors variously depending on hydrological periods. Ecol Indic 101:41–49. https://doi.org/10.1016/j.ecolind.2018.12.049
Qu X, Chen Y, Liu H, Xia W, Lu Y, Gang D, Lin L (2020) A holistic assessment of water quality condition and spatiotemporal patterns in impounded lakes along the eastern route of China’s South-to-North water diversion project. Water Res 185:116275. https://doi.org/10.1016/j.watres.2020.116275
Qu Y, Wu N, Guse B, Fohrer N (2022) Distinct indicators of land use and hydrology characterize different aspects of riverine phytoplankton communities. Sci Total Environ 851:158209. https://doi.org/10.1016/j.scitotenv.2022.158209
Qu X, Olden JD, Xia W, Liu H, Xie Z, Hughes RM, Chen Y (2023) Hydrology and water quality shape macroinvertebrate patterns and facilitate non-native species dispersals in an inter-basin water transfer system. J Environ Manage 329:117111. https://doi.org/10.1016/j.jenvman.2022.117111
Reynolds CS (1994) The long, the short and stalled: on the attributes of phytoplankton selected by physical mixing in lakes and rivers. Hydrobiologia 289:9–21. https://doi.org/10.1007/BF00007405
Reynolds CS (2000) Hydroecology of river plankton: the role of variability in channel flow. Hydrol Process 14:3119–3132. https://doi.org/10.1002/1099-1085(200011/12)14:16/17%3c3119::AID-HYP137%3e3.0.CO;2-6
Reynolds CS (2006) The ecology of phytoplankton: ecology, biodiversity and conservation. Cambridge University Press, Cambridge
Reynolds CS, Huszar VLM, Kruk C, Naselli-Flores L, Melo S (2002) Towards a functional classification of the freshwater phytoplankton. J Plankton Res 24:417–428. https://doi.org/10.1093/plankt/24.5.417
Rusanov AG, Bíró T, Kiss KT, Buczkó K, Grigorszky I, Hidas A, Duleba M, Trábert Z, Földi A, Ács É (2022) Relative importance of climate and spatial processes in shaping species composition, functional structure and beta diversity of phytoplankton in a large river. Sci Total Environ 807:150891. https://doi.org/10.1016/j.scitotenv.2021.150891
Salmaso N, Braioni MG (2008) Factors controlling the seasonal development and distribution of the phytoplankton community in the lowland course of a large river in Northern Italy (River Adige). Aquat Ecol 42:533–545. https://doi.org/10.1007/s10452-007-9135-x
Shoener BD, Schramm SM, Béline F, Bernard O, Martínez C, Plósz BG, Snowling S, Steyer J-P, Valverde-Pérez B, Wágner D (2019) Microalgae and cyanobacteria modeling in water resource recovery facilities: a critical review. Water Res X 2:100024. https://doi.org/10.1016/j.wroa.2018.100024
Sommer U, Adrian R, Domis LDS, Elser JJ, Gaedke U, Ibelings B, Jeppesen E, Lürling M, Molinero JC, Mooij WM, Donk EV, Winder M (2012) Beyond the plankton ecology group (PEG) model: mechanisms driving plankton succession. Annu Rev Ecol Evol Syst 43:429–448. https://doi.org/10.1146/annurev-ecolsys-110411-160251
Song J, Hou C, Liu Q, Wu X, Wang Y, Yi Y (2020) Spatial and temporal variations in the plankton community because of water and sediment regulation in the lower reaches of Yellow River. J Clean Prod 261:120972. https://doi.org/10.1016/j.jclepro.2020.120972
Song Y, Guo Y, Liu H, Zhang G, Zhang X, Thangaraj S, Sun J (2022) Water quality shifts the dominant phytoplankton group from diatoms to dinoflagellates in the coastal ecosystem of the Bohai Bay. Mar Pollut Bull 183:114078. https://doi.org/10.1016/j.marpolbul.2022.114078
Stoffers T, Buijse AD, Geerling GW, Jans LH, Schoor MM, Poos JJ, Verreth JAJ, Nagelkerke LAJ (2022) Freshwater fish biodiversity restoration in floodplain rivers requires connectivity and habitat heterogeneity at multiple spatial scales. Sci Total Environ 838:156509. https://doi.org/10.1016/j.scitotenv.2022.156509
Sun X, Wang W (2021) The impact of environmental parameters on phytoplankton functional groups in northeastern China. Ecol Eng 164:106209. https://doi.org/10.1016/j.ecoleng.2021.106209
Tan Q, Ma X, Li B, Lu H, Fu M, Yao W (2017) Ecological health assessment of the upper reaches of the Yangtze River, based on biotic integrity index of phytoplankton. Freshwater Fisheries 47:97–104. https://doi.org/10.13721/j.cnki.dsyy.2017.03.015
Thorp JH, Delong MD (1994) The riverine productivity model: anheuristic view of carbon sources and organic processing in large river ecosystems. Oikos 70:305–308. https://doi.org/10.2307/3545642
Tian Y, Jiang Y, Liu Q, Xu D, Liu Y, Song J (2021) The impacts of local and regional factors on the phytoplankton community dynamics in a temperate river, northern China. Ecol Indic 123:107352. https://doi.org/10.1016/j.ecolind.2021.107352
Tong Y, Bu X, Chen J, Zhou F, Chen L, Liu M, Tan X, Yu T, Zhang W, Mi Z, Ma L, Wang X, Ni J (2017) Estimation of nutrient discharge from the Yangtze River to the East China Sea and the identification of nutrient sources. J Hazard Mater 321:728–736. https://doi.org/10.1016/j.jhazmat.2016.09.011
Vörösmarty CJ, McIntyre PB, Gessner MO, Dudgeon D, Prusevich A, Green P, Glidden S, Bunn SE, Sullivan CA, Liermann CR, Davies PM (2010) Global threats to human water security and river biodiversity. Nature 467:555–561. https://doi.org/10.1038/nature09440
Wang J, Liu Q, Zhao X, Borthwick AGL, Liu Y, Chen Q, Ni J (2019) Molecular biogeography of planktonic and benthic diatoms in the Yangtze River. Microbiome 7:153. https://doi.org/10.1186/s40168-019-0771-x
Wang Y, Cai Y, Yin X, Yang Z (2020) Succession of phytoplankton functional groups in Macau’s two shallow urban border reservoirs under multiple changing factors. J Clean Prod 264:121553. https://doi.org/10.1016/j.jclepro.2020.121553
Wang C, Jia H, Wei J, Yang W, Gao Y, Liu Q, Ge D, Wu N (2021) Phytoplankton functional groups as ecological indicators in a subtropical estuarine river delta system. Ecol Indic 126:107651. https://doi.org/10.1016/j.ecolind.2021.107651
Wu Z, Cai Y, Liu X, Xu C, Chen Y, Zhang L (2013) Temporal and spatial variability of phytoplankton in Lake Poyang: the largest freshwater lake in China. J Great Lakes Res 39:476–483. https://doi.org/10.1016/j.jglr.2013.06.008
Wu Z, Liu J, Huang J, Cai Y, Chen Y, Li K (2019) Do the key factors determining phytoplankton growth change with water level in China’s largest freshwater lake? Ecol Indic 107:1–8. https://doi.org/10.1016/j.ecolind.2019.105675
Wu N, Guo K, Suren AM, Riis T (2023a) Lake morphological characteristics and climatic factors affect long-term trends of phytoplankton community in the Rotorua Te Arawa lakes, New Zealand during 23 years observation. Water Res 229:119469. https://doi.org/10.1016/j.watres.2022.119469
Wu Z, Wang F, Wang X, Li K, Zhang L (2023b) Water quality assessment using phytoplankton functional groups in the middle-lower Changjiang River, China. Limnologica 99:126056. https://doi.org/10.1016/j.limno.2023.126056
Xiao L, Wang T, Hu R, Han B, Wang S, Qian X, Padisák J (2011) Succession of phytoplankton functional groups regulated by monsoonal hydrology in a large canyon-shaped reservoir. Water Res 45:5099–5109. https://doi.org/10.1016/j.watres.2011.07.012
Xiong F, Olden JD, Lu Y, Liu H, Qu X, Xia W, Guo C, Wu X, Infante DM, Wang L, Chen Y (2021) Riparian land use and in–channel stressors drive fish community structure in the Yangtze River. Landscape Ecol 36:3079–3095. https://doi.org/10.1007/s10980-021-01278-8
Xiong F, Chen Y, Zhang S, Xu Y, Lu Y, Qu X, Gao W, Wu X, Xin W, Gang D, Lin L (2022) Land use, hydrology, and climate influence water quality of China’s largest river. J Environ Manage 318:115581. https://doi.org/10.1016/j.jenvman.2022.115581
Xiong F, Infante DM, Olden JD, Gao W, Wang L, Chen Y (2023) River–lake connectivity, wetland, and human stress factors shape fish diversity (alpha and beta) patterns in the middle and lower Yangtze River, China. Landscape Ecol 38:3809–3824. https://doi.org/10.1007/s10980-023-01616-y
Xu H, Paerl HW, Qin B, Zhu G, Gao G (2010) Nitrogen and phosphorus inputs control phytoplankton growth in eutrophic Lake Taihu, China. Limnol Oceanogr 55:420–432. https://doi.org/10.4319/lo.2010.55.1.0420
Yang W, Zheng Z, Zheng C, Lu K, Ding D, Zhu J (2018) Temporal variations in a phytoplankton community in a subtropical reservoir: an interplay of extrinsic and intrinsic community effects. Sci Total Environ 612:720–727. https://doi.org/10.1016/j.scitotenv.2017.08.044
Yang C, Nan J, Li J (2019) Driving factors and dynamics of phytoplankton community and functional groups in an estuary reservoir in the Yangtze River, China. Water 11:1184. https://doi.org/10.3390/w11061184
Yang Y, Chen H, Al MA, Ndayishimiye JC, Yang J, Isabwe A, Luo A, Yang J (2022) Urbanization reduces resource use efficiency of phytoplankton community by altering the environment and decreasing biodiversity. J Environ Sci 112:140–151. https://doi.org/10.1016/j.jes.2021.05.001
Yates AG, Bailey RC, Schwindt JA (2007) Effectiveness of best management practices in improving stream ecosystem quality. Hydrobiologia 583:331–344. https://doi.org/10.1007/s10750-007-0619-4
Yin C, He W, Guo L, Gong L, Yang Y, Yang J, Ni L, Chen Y, Jeppesen E (2022) Can top-down effects of planktivorous fish removal be used to mitigate cyanobacterial blooms in large subtropical highland lakes? Water Res 218:118483. https://doi.org/10.1016/j.watres.2022.118483
Zhang X, Xie P, Chen F, Li S, Qin J (2007) Driving forces shaping phytoplankton assemblages in two subtropical plateau lakes with contrasting trophic status. Freshw Biol 52:1463–1475. https://doi.org/10.1111/j.1365-2427.2007.01776.x
Zhang M, Shi X, Yang Z, Yu Y, Shi L, Qin B (2018a) Long-term dynamics and drivers of phytoplankton abundance in eutrophic Lake Taihu. Sci Total Environ 645:876–886. https://doi.org/10.1016/j.scitotenv.2018.07.220
Zhang Y, Wang R, Qu X, Xia W, Xin W, Guo C, Chen Y (2018b) Effects of aquaculture on phytoplankton communities of lakes in the middle reach of the Yangtze River Basin. Acta Hydrobiol Sin 42:1135–1143. https://doi.org/10.7541/2018.139
Zhang Y, Peng C, Huang S, Wang J, Xiong X, Li D (2019) The relative role of spatial and environmental processes on seasonal variations of phytoplankton beta diversity along different anthropogenic disturbances of subtropical rivers in China. Environ Sci Pollut Res 26:1422–1434. https://doi.org/10.1007/s11356-018-3632-4
Zhao G, Pan B, Li Y, Zheng X, Zhu P, Zhang L, He H (2020) Phytoplankton in the heavy sediment-laden Weihe River and its tributaries from the northern foot of the Qinling Mountains: community structure and environmental drivers. Environ Sci Pollut Res 27:8359–8370. https://doi.org/10.1007/s11356-019-07346-6
Zhou Q, Zhang Y, Lin D, Shan K, Luo Y, Zhao L, Tan Z, Song L (2016) The relationships of meteorological factors and nutrient levels with phytoplankton biomass in a shallow eutrophic lake dominated by cyanobacteria, Lake Dianchi from 1991 to 2013. Environ Sci Pollut Res 23:15616–15626. https://doi.org/10.1007/s11356-016-6748-4
Zhu A, Hu J, Li S, Zhou L, Liang Y (2013a) Phytoplankton diversity and water quality in the main stream and tributaries of Three Gorges Reservoir region of Yangtze River during dry seasons. J Lake Sci 25:378–385. https://doi.org/10.18307/2013.0311
Zhu K, Bi Y, Hu Z (2013b) Responses of phytoplankton functional groups to the hydrologic regime in the Daning River, a tributary of Three Gorges Reservoir, China. Sci Total Environ 450–451:169–177. https://doi.org/10.1016/j.scitotenv.2013.01.101
Acknowledgements
We thank many students and field staff who assisted in this study's field sampling and laboratory analyses.
Funding
The current study was mainly supported by National Key Research and Development Program of China (2023YFC3209002; 2019YFD0901203) and Chinese Academy of Sciences (ZDRW-ZS-2017-3-2). HW and GF were also supported by the Innovative and Entrepreneurial Talents Project of Jiangxi Province (JXSQ2020102109).
Author information
Authors and Affiliations
Contributions
YC designed the study. WG, FX, and YL collected the data. WG analyzed the data. WG, FX, YL, WX, HW, GF, CK, LF, XG, and YC wrote the manuscript. All authors contributed to revising, commenting, and finalizing the manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1.
Supplementary tables.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Gao, W., Xiong, F., Lu, Y. et al. Water quality and habitat drive phytoplankton taxonomic and functional group patterns in the Yangtze River. Ecol Process 13, 11 (2024). https://doi.org/10.1186/s13717-024-00489-6
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s13717-024-00489-6