Abstract
Medicinal and aromatic plants possess pharmacological properties (antidiabetes, anticancer, antihypertension, anticardiovascular, antileprosy, etc.) because of their potential to synthesize a wide range of therapeutic bioactive secondary metabolites. The concentration of bioactive secondry metabolites depends on plant species, local environment, soil type and internal microbiome. The internal microbiome of medicinal plants plays the crucial role in the production of bioactive secondary metabolites, namely alkaloids, steroids, terpenoids, peptides, polyketones, flavonoids, quinols and phenols. In this review, the host specific secondry metabolites produced by endophytes, their therapeutic properties and host-endophytes interaction in relation to production of bioactive secondry metaboloites and the role of endophytes in enhancing the production of bioactive secondry metabolites is discussed. How biological nitrogen fixation, phosphorus solubilization, micronutrient uptake, phytohormone production, disease suppression, etc. can play a vital role in enhacing the plant growth and development.The role of endophytes in enhancing the plant growth and content of bioactive secondary metabolites in medicinal and aromatic plants in a sustainable mode is highlighted.
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Introduction
Medicinal and aromatic plants have the capability to synthesize different types of bioactive secondary metabolites (Cushnie et al. 2014) that are significant to endure and prosper in the indigenous habitat, incorporating defensive capacities as for abiotic and biotic stresses (Vardhini and Anjum 2015). The ''bioactive'' secondary metabolites are derived from intermediates of primary metabolites and are not essential for growth and development of organisms (Tiwari and Rana 2014). There are five main classes of secondary metabolites produced by plants and microbes such as terpenoids and steroids, fatty acid-derived substances and polyketides, alkaloids, nonribosomal polypeptides, and enzyme cofactors (Erb and Kliebenstein 2020). These biologically active secondary metabolites possess pharmaceutical properties and are used in the treatment of skin disorders, cancer, hypertension, cardiovascular diseases, diabetes, leprosy, thyroid, obesity etc. (Olasehinde et al. 2017; Seca and Pinto 2018; Shayganni et al. 2016). The biologically active secondary metabolites are species and environment specific, therefore, their production and composition depends on plant species, soil types and relationship with microorganisms (Morsy 2014). The communities of plants microbiota and their physiological functions are also affected by bioactive secondary metabolites produced by several medicinal and aromatic plants (Chaparro et al. 2014).
Earlier research focused on bioactive secondary metabolites of medicinal plants only, however, now the focus is shifting towards its microbiome as a large number of bioactive secondary metabolites are actually produced by associated microbes or through their interaction with the host plant. The endophytic microorganisms have crucial role in the production of bioactive secondary metabolites such as- steroids, alkaloids, peptides, polyketones, terpenoids, flavonoids, and phenols (Matsumoto and Takahashi 2017; Pratiwi et al. 2018). Bioactive secondary metabolites also have certain agricultural, industrial and medical applications (Aswani et al. 2020; Daud et al. 2019). It is believed that the phytochemical constituents of plants are related either directly or indirectly to endophytic microbes and their interactions with host plants (Qi et al. 2012). Egamberdieva et al. (2017a) reported that antimicrobial activity of medicinal and aromatic plants correlates with the proportion of antagonistic endophytes.
Apart from producing secondary metabolites on its own, these endophytes may also associate with plants to accelerate the biosynthesis of bioactive compounds secreted by them. The synergistic effect of endophytic microorganisms and medicinal plants have been investigated as a source of crude products with high medicinal potential as compared to plants alone (Hardoim et al. 2015; Heinig et al. 2013). Likewise, Strobel (2003) reported that endophytes associated with medicinal plants produces a wide varieties of secondary metabolites as compared to them alone. Ding et al. (2018) reported that endophytic fungi- Aspergillus sp., Fusarium sp. and Ramularia sp. of Rumex gmelini Turcz increased the production of bioactive compounds in the host plant and showed similarity with them in relation to production pattern of secondary metabolites.
Rapid urbanization and industrialization due to a gradual increase in the world population have put immense pressure on the available cultivated land for accelerated crop production and productivity. To achieve this, excessive and irrational use of agrochemicals such as fertilizers, herbicides, fungicides, and other supplements has been adopted. However, continuous use of these agrochemicals for enhanced soil fertility, productivity and plant production has resulted in adverse impacts on the ecosystem, including pollution of soil, groundwater, and aquifers (Bohlool et al. 1992; Byrnes 1990; Mulongoy et al. 1991; Zhu and Chen 2002). Hence, eco-friendly methods are need of the hour to maintain the quality of soil, water and other habitats of living organisms to keep sustained crop production and ecological stability. Plant growth-promoting substances are produced in huge amounts by these rhizospheric and endophytic microbes that influences the overall growth and development of medicinal and aromatics plants directly or indirectly (Larkin and Tavantzis 2013; Sharma et al. 2020). Endophytes enhances the plant growth and development through different mechanisms like- nutrient acquisition, biological control of phytopathogens and tolerance to abiotic stress (Berg et al. 2014; Egamberdieva et al. 2011; Malfanova et al. 2011). Reports suggest that microbes enhance the survivability of plants by modulating biosynthetic activities involved in accumulation of stress tolerant molecules (proline, glycine betaine, etc.) and antioxidant enzymes (catalase, peroxidase, glutathione reductase, superoxide dismutase, etc.) in plants body under stress conditions (Hussain et al. 2014; Tiwari et al. 2016; Vardharajula et al. 2011).
The objective of this review is (1) to reveal plant- and microbe-derived constituents of medicinal plants; (2) to discuss host endophytic interaction in relation to modulation of biosynthesis of bioactive secondary metabolites in medicinal plants, (3) to highlight possible mechanisms of plant growth promotion of medicinal plants by endophytes.
Bioactive secondary metabolites and endophytes
Endophytic fungi, have the potential to produce similar class and even in some cases exactly the same secondary metabolites that are pharmacologically active as their hosts (Venieraki et al. 2017). This relationship of endophytic fungi with its host is certainly more complex than it appeared initially. It was proposed that the horizontal transfer of genes responsible for the production of these bioactive compounds might have been introduced from the host. However, the genome sequencing showed otherwise, as the corresponding genes differ significantly among the host and endophytic fungi and might have evolved independently (Lu et al. 2019). Inspecting the mechanisms underlying the synthesis of endophyte mediated plant metabolites plays an important role in exploring these endophytes for the production of new bioactive metabolites commercially, especially the ones produced by the plants.
Host plant specific therapeutics bioactive secondary metabolites produced by endophytes
Belonging to the group of natural products with extreme diversity, secondary metabolites are organic compounds synthesized by microorganisms, plants and animals that are not associated with the organism’s normal growth and development. These metabolites are often synthesized to their maximum potential mostly during the stationary phase. The organisms producing secondary metabolites can survive in their absence, thus making them non-essential for immediate survival. However, the secondary metabolites are also essential for cellular metabolism of organism and dependent on primary metabolites for synthesis of important enzymes, substrates and other molecules necessary for long term host survival (Roze et al. 2011). Secondary metabolites are often categorized into different classes and among them most are classified on the basis of their biosynthetic origin like alkaloids, steroids, terpenoids, peptides, polyketones, flavonoids, quinols and phenols (Matsumoto et al. 2017; Pratiwi et al. 2018).
Plants being an excellent source of numerous bioactive compounds, especially the medicinal plants, have been used in traditional medicines for treatment of several diseases and are basis for discovery and advancement of modern therapeutics (Pan et al. 2013). For primary healthcare, nearly 80% population is absolutely reliant on herbal drugs in developing countries and in the last four decades, more than 51% of the small molecule therapeutics approved were natural product based, with rest being produced synthetically. This ever-growing demand for herbal medicines and naturally produced healthcare products, emphasizes the rapidly growing use of medicinal plants (Chen et al. 2016).
Earlier studies on medicinal plants were mainly focused on their constituents, however, with advancement in technology the paradigm has been shifted to structural and functional attributes of microbiomes associated with the host medicinal plants. Surprisingly, it was observed that not only the host plants but also their associated microbial population, plant endophytes in particular, were able to produce the plant therapeutic compounds (Table 1). Ever since the report on Taxomyces andreanae, an endophyte of Taxus brevifolia, producing a bioactive secondary metabolite similar to its host (Stierle et al. 1993), several plant-derived bioactive secondary metabolites have been reported to be synthesized by the host endophytes (Zhao et al. 2011). Recent studies have also shown that microbes or their interaction with host produces several important natural products and in several cases involving medicinal plants it is assumed that microbiome associated with plant, especially endo-microbiome, is significantly involved in bioactive compound production from the plant either directly or indirectly (Miller et al. 2012). The metabolism of associated microbiome and its interaction with host highly influences and regulates the quality, growth and health of medicinal plants. Even the ability of phytotherapeutics to suppress human pathogens can be attributed to these medicinal plant associated microbiome and their metabolites (Miller et al. 2012; Mousa and Raizada 2013).
Endophytic bacteria are the potential source of numerous secondary metabolites that have applications in many plant growth and development aspects like antibiotics, antiparasitics, antioxidants, plant growth promoting compounds and enzymes, etc. There are various reports suggesting the role of host-specific endophytic bacteria in producing the bioactive compounds with antimicrobial activities. The antibacterial activity of endophytic Bacillus and Lysinibacillus species isolated from African Combretum molle was recorded against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus (Diale et al. 2018). Leaf and root endophytes of Raphanus sativus and in another study root endophytes of Zingiber officinale were shown to exhibit antibacterial activity (Taechowisan et al. 2013). There have been reports where cell-wall degrading enzymes from endophytic Micrococcus sp., Bacillus sp., and P. polymyxa isolated from Panax ginseng and Plectranthus tenuiflorus have been shown to exhibit antimicrobial activity (El-Deeb et al. 2013). The other endophytic bacteria such as Paenibacillus denitriformis, Bacillus pseudomycoides and B. licheniformis have been reported to produce L-asparaginase enzyme efficiently (Joshi and Kulkarni 2016). This enzyme has major role in chemotherapy as it catalyses the L-asparagine conversion (Jiang et al. 2018).
Actinomycetes has been well documented for their contribution in antibiotic development and also other bioactive metabolites production. This potential of actinomycetes to synthesize bioactive secondary metabolites is also being studied for their endophytic forms. The endophytic actinomycetes of Chinese medicinal and mangrove plants reported to exhibit antibacterial activity against Enterococcus faecalis, S. aureus, Klebsiella pneumoniae, E. coli, Acinetobacter baumannii and P. aeruginosa. Among these, some were even resistant to antibiotics like methicillin, carbapenem and vancomycin (Jiang et al. 2018). The characterization of several metabolites isolated from endophytic Streptomyces species has been found to exhibit various activities like antibacterial, antimalarial and antifungal (Ek-Ramos et al. 2019). Similarly, endophytic actinomycetes from Iranian medicinal plants, Kennedia nigriscans and Malaysian plants has been seen to exhibit antimicrobial activity against various bacterial pathogens (Fikri et al. 2018). Streptomyces sp. (strain SUK06), an endophyte isolated from Thottea grandiflora (Malaysian medicinal plant), has been reported to inhibit the growth of methicillin-resistant S. aureus (MRSA) (Ghadin et al. 2008).
The endophytic fungi also produces a wide range of these bioactive secondary metabolites with vital functions, exhibiting valuable medicinal and antibiotic properties. Numerous fungal endophytes has been reported to display host specificity in terms of metabolite synthesis. For example, out of the 27 fungal strains obtained from Ginkgo biloba trees bark, only one strain i.e. Fusarium oxysporum SY0056 was able to synthesize Ginkgolide B (Cui et al. 2012). Similarly, Pestalotiopsis uvicola GZUYX13 from Ginkgo biloba leaves, was the only isolate among the 57 strains obtained from different plant parts including root, stem and leaf that was able to produce bilobalide metabolite (Qian et al. 2016). Both these metabolites have been very well documented to have therapeutic roles with the former being involved in cardiovascular or respiratory system disorder while the later having neuroprotective effects (Kiewert et al. 2008; Usai et al. 2011). In another study, out of 11 fungal strains examined for Vincamine indole alkaloids production, isolated from roots and stems of Nerium indicum, only one fungal strain (CH1) was able to produce vincamine alkaloids similar to its host. These alkaloids are reported to show therapeutic properties like cerebrovascular prevention, vascular dementia reduction, etc. (Vora et al. 2013). Moreover, the fungal endophytes of Rheum palmatum (You et al. 2013) and Forsythia suspense (Qu et al. 2008) were reported to produce bioactive compounds like rhein and emodin, and Phillyrin, respectively. These compounds have major applications in alleviating pain and anti-inflammatory properties.
Host-endophytes interaction in relation to production of therapeutic secondary metabolites by medicinal plants
Recently, investigation on the endophytic microorganisms has increased owing to their intimate association with the host. From the conventional point of view, the quantity and quality of the medicinal plant based crude drugs depends largely on the genotypic characters and ecological habitat of the plant and the nutrient status of soil (Dai et al. 2003; Sherameti et al. 2005). Furthermore, this has been recognized gradually in the recent years that endophytic microorganisms have been one of the major drivers in regulating the quantity and quality of crude drugs via host-specific interactions, thus making it indispensable to understand in-depth relationship of endophytic microorganisms with the medicinal plants in order to promote or enhance the production of crude drugs.
Studies revealed that, endophytes do produce secondary metabolites that are either identical or similar to their hosts. Some of these bioactive compounds which are produced by endophyte and host collaboration includes anticancer drugs like podophyllotoxin and camptothecin (Puri et al. 2006) and azadirachtin as natural insecticides (Kusari et al. 2012). There have been numerous mechanisms reported for simultaneous synthesis of biological compounds. However, in some instances, the biosynthetic mechanism completely differs in plants and their endophytes even for the same compound (Bomke et al. 2009). The horizontal gene transfer between the endophytes and their host has been hypothesized as one of the possible mechanisms, though it has been restricted to microorganisms (Taghavi et al. 2005). So, one thing is clear and can be strongly put forward that the interactions between the host and their endophytes significantly regulates the co-production of these bioactive compounds (Heinig et al. 2013).
Recently, significant attention has been given to endophytes by the microbial chemistry community owing to their contribution for the discovery of novel bioactive metabolites. And, it has also been reported that this intimate association of endophytes with their host was more promising in production of higher number and diversity of the bioactive compounds as compared to non-endophytes like epiphytes of rhizospheric microorganisms (Strobel et al. 2003). The relationship being symbiotic in nature is likely to give compounds with reduced cell toxicity. This particular attribute is of prime importance for medical community as these compounds may not adversely affect the eukaryotic systems.
Taxol, a multibillion-dollar anticancer drug, is one of the major success story of endophytic natural products. Initially, this compound was isolated from a traditional medicinal plant Taxus brevifolia (Pacific yew tree) (Wani et al. 1971). After that numerous plants other than this have been reported to produce taxol. The endophytic fungi from Taxomyces andreanae has been investigated to produce this compound (Stierle et al. 1995). Considering the mechanism behind its biosynthesis in the host, it has been suggested that the genome of endophytes has no significant homology with Taxus sp. for the production of taxol (Heinig et al. 2013), demonstrating the independent development of taxol biosynthesis in endophytes. Nevertheless, this example supports the rationale that traditional medicinal plants can be used as the starting point to investigate endophytes for their production of biologically active compounds. As stated earlier, approximately 70% of anti-infectives or their derivatives are naturally produced. However, instead of synthesising these derivatives through combinatorial chemistry, their biosynthesis at genetic level has been elucidated. These synthesis of natural products are regulated mostly by single gene clusters, therefore efforts are carried out in the direction to utilize these clusters through genetic engineering for biosynthesis of these natural compounds and also their derivatives. Figure 1 shows host endophytes interaction in relation to modulation of biosynthesis of bioactive secondry metabolites.
Induction of metabolic activities of host plant
Induction of host secondary metabolism by the endophytes has been dealt less when compared to the endophyte metabolism induction, however, phenolic compounds related to defense in roots infected with endophytes have been studied long ago and are very well reported (Schulz et al. 1999). Induction of phenolic compound by infection of endophytes in Lolium perenne has been reported. Some of the major compounds that induced the antioxidant potential of plant extracts upon colonization of endophytes include chlorogenic acids, hydroxycinnamic acids and glycosylated flavonoids (Qawasmeh et al. 2012). Although these metabolic changes can be reduced or even remain unaltered upon infection, but scenario changes when the host interacts with endophytic toxic compounds. The toxicity to plants can be attributed to the herbicidal activity of endophytes. In a study, it was shown that leucinostatin A, a peptide derived from endophyte, was glycosylated in Taxus species, whereas it was still toxic to the non-host plants (Strobel et al. 1997). These toxic compounds may be used to regulate the selectivity of plant–microbe interrelationship.
Effect of host plant on metabolic pattern of endophytes
As discussed earlier, host plant metabolism is influenced by their endophytes, and vice versa. It can also be speculated that alteration of secondary metabolite patterns in endophytes is influenced by their host range. The regulation of pathogenic fungi metabolite patterns by the host plant has also been reported. Heterobasidium species showed different metabolic pattern for infecting and non-infecting pine species (Hansson et al. 2014).
Modification of metabolisms of endophytes inside the host plant
Metabolism of bioactive metabolites from host and their biosynthesis by endophytes are the most fascinating areas, however, meagre information is available. In recent times, the ability to synthesize bioactive metabolites by endophytes has gained momentum, corresponding to the constant discovery of these endophytic fungi capable of producing plant compounds. As mentioned earlier also, so far synthesis of Taxol (paclitaxel) by endophytes is the most notable example for synthesis of plant metabolite partially. Owing to its important medical use as anti-cancer drug, its production on large scale in cell cultures has been optimized, however, one has to look for alternative sources other than plants to meet the ever growing demands for this drug (Cusido et al. 2014; Heinig et al. 2013; Malik et al. 2011).
The discovery of Taxol production in T. andreanae, an endophytic fungus of Taxus brevifolia was succeeded by the isolation of other endophytic fungus from a variety of Taxus sp. (Yuan et al. 2006; Zhang et al. 2009). After that, several other fungi, isolated from Taxus baccata and non-Taxus plants, were reported for the production of microbial taxane including Aspergillus, Alternaria, Fusarium, Cladosporium, Monochaetia, Pestlotia, Pestalotiopsis, Pithomyces, Penicillium and Xylaria. However, upon re-examination of taxane biosynthesis by fungi and host plant revealed no independent biosynthesis of taxane in the endophytes (Heinig et al. 2013). On the other hand, different bioactive metabolites including torreyanic acid also having anticarcinogenic properties were found in an endophyte, Pestalotiopsis microspora, isolated from other species of Taxaceae (Lee et al. 1996). In similar findings, endophytes were reported to produce different compounds in hosts from unrelated families like podophyllotoxin from Berberidaceae and rohitukine from Meliaceae, as precursor for another cancer drug (Müller et al. 2015). In some cases, like podphyllotoxin from Podophyllum, the sustainable production of such valuable bioactive compounds has not yet been achieved, though Trametes hirsute, a novel fungal endophyte, has shown promising approach for synthesis of this compound (Puri et al. 2006). Synthesis of insecticidal compounds like azadirachtin A and B from Eupenicillium parvum of Azadirachta indica has also been reported (Kusari et al. 2012). These examples, of course doesn’t rule out the endophytes existence on respective host plants.
So, far the examples involved fungi as endophytes, however, the bacterial endophytes also share similar interactions with their host. Bioactive metabolites of oxylipin family with antimicrobial properties have been reported from stem extracts of Alternanthera brasiliana (Trapp et al. 2015). Several among these metabolites were also found in the genus Bacillus isolated from Alternathera plants and it was speculated that these compounds of oxylipin family obtained by the host from their bacterial endophytic counterparts shared some similarity (Trapp et al. 2015).
Biotransformation of host compounds by endophytes
Paraconiothyrium variabile, a leaf endophyte isolated from a medicinal plant Cephalotaxus harringtonia, has been reported to metabolize the metabolites of its host plant. In-depth analysis and monitoring of these compounds and structural characterization revealed beneficial effects of the fungal endophyte. The endophyte could transform the glycosylated flavonoids, leading to aglycone compound production in the host which significantly induced the hyphal growth from spores (Tian et al. 2014). This study strongly depicts an underlying chemical co-operation between the host and the endophyte. In another study the symbiotic fungi Colletotrichum tropicale in cucumber plant changed the leaf chemistry by altering the host metabolism such that the leaf cutting by ants was reduced to almost half in the colonized plants as compared to non-colonized plants. Thus, the endophytes could even alter the plant–insect interaction (Estrada et al. 2013). Besides this, endophytes has also been reported to show the detoxification of host defense compounds. A toxic compound called benzoxazinoids is synthesized by maize plants for its protection from other pests. However, the enzymes synthesized by endophyte metabolizes this compound via deglycosylation resulting in a non-toxic product (Saunders and Kohn, 2008). Not only this, the detoxification of benzoxazinoids also leads to enhanced colonization by other non-tolerant fungal endophytes. The other mechanisms applied by endophytes to detoxify certain toxic plant compounds includes nitration, hydrolysis, acylation, reduction and oxidation (Zikmundova et al. 2002).
Modulation of phytochemistry of medicinal plants by endophytes
Apart from the biosynthetic mechanisms and interactions mentioned above regarding the bioactive metabolites, endophytes have also been observed to play a major role in improving the biosynthesis of host metabolites (Table 2). In a study, when Catharanthus roseus plants were inoculated with Choanephora infundibulifera and Curvularia sp., it was observed that the content of a terpenoids indole alkaloid (TIA) was increased by 229–403%. There was a significant upregulation in regulatory and structural genes involving biosynthetic pathway of TIA in endophyte inoculated plants, as evident from real-time PCR results (Pandey et al. 2016). In a similar study, biosynthesis of tanshionones was enhanced and related genes were upregulated by application of polysaccharide fraction from an endophyte Trichoderma atroviride in host plant Salvia miltiorrhiza (Ming et al. 2013). From correlative transcriptome and metabolome analysis, this can be further suggested that endophytes can even reprogram the host metabolism by favouring secondary metabolism over the primary one (Dupont et al. 2015).
Like the fungal partners, bacterial endophytes have also been reported to exhibit potential for producing several bioactive metabolites with profound effect on both primary and secondary metabolism of the host. Upon inoculation of poplar plants with Paenibacillus sp., levels of urea, threitol and asparagine increased significantly whereas several organic acids, sugar phosphates and amino acids were reduced (Scherling et al. 2009). Likewise, inoculation of grapevine with an endophyte Enterobacter ludwigii significantly increased the level of vanillic acid while reducing several others including ampellopsin, catechin, isohopeaphenol, esculin, etc. (Lòpez-Fernàndez et al. 2016).
Several studies involving medicinal plants, Arabidopsis thaliana, food crops, trees/shrubs and ornamentals were conducted to understand the effect of bacteria on its phytochemistry. Among them, medicinal and crop plants were investigated in detail, and comprehensive information has been reviewed to know the mechanism behind the bacteria-mediated alterations or regulations in host plants. Next comes the herbs in which the families Lamiaceae, Asteraceae and Fabaceae were the dominating ones studied in which Bacillales and Pseudomonadales were the most investigated bacterial orders followed by Actinomycetales.
The main purpose of study with the medicinal plants was to explore the potential of endophytic bacteria to regulate the level of bioactive or specific medicinal metabolite(s). Upregulation of cytochrome P450 oxidoreductase and monooxygenase genes was observed in Artemisia annua induced by the endophyte Pseudonocardia sp.. These genes were responsible for biosynthesis of artemisinin, an antimalarial agent (Li et al. 2012). In another study, two endophytic bacteria, Micrococcus sp. and Staphylococcus sciuri have reported to boost the production of metabolites like serpentine, ajmalicine and vindoline, having therapeutic applications, in Catharanthus roseus (Tiwari et al. 2013). Similarly, many other major endophytic bacteria including Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens, Paenibacillus polymyxa, etc. have been reported to enhance the production and accumulation of important secondary metabolites in the host (Del Giudice et al. 2008; Gao et al. 2015).
Additionally, the root endophytes have also been suggested to play an important role in transformation of plant metabolites to various other derivatives depending on the interaction. For example, root-associated bacteria was found in the essential oil producing parenchymatous cells of Vetiver plant where they metabolized the oil sesquiterpenes to other new compounds that were either absent or present in least amounts in raw oil (Del Giudice et al. 2008). Similarly, the vetiver plantlets inoculated in-vitro with root-associated bacteria produced oils with marked differences in composition as compared to the plantlets in-vivo. It was further reported that these group of bacteria significantly induced the plant terpene synthase gene expression (Del Giudice et al. 2008). Besides this the bacterial endophyte, Burkholderia sp., from ginseng roots transforms ginsenoiside Rb1 to potent antitumor form Rg3 (Fu et al. 2017). Accordingly, it can be further suggested from these studies that the endophytic bacteria besides enhancing the amount of particular bioactive metabolites in their host, also executed the biotransformation of less active compounds to active by-products. Interestingly, it was observed that an important metabolite maytansine, an antitumor agent, is biologically synthesized by community of root endophytic bacteria in Putterlickia retrospinosa and P. verrucosa plants, which was initially presumed to be of plant origin (Kusari et al. 2014).
The research conducted to explore, analyse and understand the underlying mechanism of bacteria-mediated phytochemical alterations are expanding due to the development in next generation sequencing and advance mass spectroscopy technologies. Future investigations on reprogramming of plant metabolome by beneficial microorganisms can henceforth be put beyond their roles as plant growth regulators or as plant protectants only. Keeping in view, their close association with the hosts than their free-living counterparts along with their promising applications, the endophytes are drawing notable attention in the present dynamics of research. It can now be established that the bioactive metabolites discovered in crude plant extracts can have various origin viz. plants, endophytes, or from synergistic effort of plant and endophytes, from endophyte mediated modification of plant metabolites and vice-versa (Etalo et al. 2018). Moreover, besides plant–microbe interaction, microbe-microbe interactions like bacterial endosymbionts in the fungal hyphae or endophyte-endophyte interactions adds to the complexity of plant metabolome, suggesting how the interplay of chemistry and genetics regulates the interactional outcomes.
Talking about the application part of endophytic bacteria, they are applied using various approaches based on the host plant growth stage and need. Among the many available methods, foliar spray and seed coating were proven more economical. There are several other endophytes that have been applied for plant growth promotions as microbial inoculants to boost up the bioactive compound synthesis in host. For example, Azotobactor chroococcum CL13 when applied to the turmeric rhizomes, saw an increase in the production of several phenolic compounds and sesquiterpenoids and curcuminoids (Kumar et al. 2014). This induction of secondary metabolites by the endophytes could be more efficient in medicinal and aromatic plants. In a related study, inoculation of two endophytic bacteria P. aeruginosa and P. pseudoalcaligenes in a medicinal plant Hyptis suaveolens increased the content of essential oils (Jha 2019). Similarly, the content of morphine and total alkaloid increased significantly in Pappaver sominiferum when Stenotrophomonas maltophilia (N5-18) was applied through foliar spray, which ultimately lead to increased productivity (Bonilla et al. 2014). The endophyte, Gilmaniella sp. AL12, have been reported to increase the herb medicinal quality of Atractylodes lancea as they interfere with the characteristic metabolites by altering the accumulation of volatile content in host (Wang et al. 2012). Synthesis of these bioactive compounds in medicinal plants, as mentioned earlier, are a reflection of genetic and biochemical alterations taking place between the endophyte and the host plant.
To date, most of the investigations involved an effect of single microbial strain on the plant metabolome. Future studies that debriefs the inter-relationships of microbiome composition and functions with dynamics of plant metabolome will greatly assist in understanding the ecological importance of microbe-derived alteration of the plant metabolome.
Plant growth promotion and endophytes
Endophytic bacteria colonise and multiply in the internal tissue of plants but do not cause disease symptoms in their hosts (Alori et al. 2017). Endophytes can promote the growth and development of medicinal plants through a variety of mechanisms (Fig. 2), including: (1) biological nitrogen fixation (BNF); (2) phosphate mobilization and solubilization; (3) siderophore production; (4) K and Zn solubilization; (5) phytohormone production; (6) production of volatile organic compounds (VOCs); (7) induction of systemic acquired resistance (SAR) and induced systemic resistance (ISR); (8) stimulating beneficial plant–microbe interaction; (9) interference with pathogen toxin production and (10) modulating the expression of stress-responsive genes in the plant biological system.
Endophytes play a significant role in plant growth promotion and biological control of plant pathogens in medicinal and aromatic plants, as shown in Tables 3 and 4. There is growing interest in using these beneficial endophytic microbes as biofertilizers and biopesticides under various abiotic and biotic stresses (Mohamad et al. 2018). The symbiotic association between endophytic microbes and plants helps each other in nutrient acquisition, water uptake and other nutrients supplements (Malfanova et al. 2011). Furthermore, many recent studies have begun to look into the importance of endophytic bacteria to medicinal plants, particularly those that grow in unusual or stressed environments (Egamberdieva et al. 2017b; Sharma et al. 2020; Vejan et al. 2016; Yan et al. 2019).
Biological nitrogen fixation
Nearly 78 percent of nitrogen is found in the atmosphere, but it is inactive, and plants cannot use it. The plant can only absorb nitrogen in its reduced form, such as ammonia or nitrate. Nitrogen is an essential primary nutrient for plants in agricultural production systems and one of the most important yield-limiting factors worldwide (Dojima and Craker 2016). Biological nitrogen fixing diazotrophs are alternative source of nitrogenous chemical fertilizers and protect the agricultural ecosystem from hazardous effects of chemiclas (Mulongoy et al. 1991).
The endophytic bacteria are ubiquitously associated with medicinal plant species and they have unique functions. Only a few studies on medicinal plants have been conducted to explore the potential of endophytes. However, recently, some studies reported that the medicinal plant Ferula songorica has been associated with huge numbers of endophytic bacteria and among them about 88% of the strains related to the BNF (Liu et al. 2017). The medicinal plant Glycyrrhiza uralensis F. has been shown to be capable of nitrogen fixation by the majority of endophytic bacterial isolates (76%) (Li et al. 2018a, b, c). Some endophytic diazotrophs, such as Azospirillum and Azotobacter have the advantage of colonising the interior plant tissue rather than the plant's surface, enabling them to better utilise the carbon source supplied by the plant (Aloo et al. 2020). Besides, endophytic diazotrophs efficiently work in the absence or low amount of oxygen in the internal biological system (stem nodes and xylem vessels) of the plant because the BNF gene- nitrogenase is sensitive to oxygen (Yan et al. 2019).
Solubilization of phosphorus
Phosphorus (P) is an essential element for plant growth and development. Phosphorus is found in soil in a huge amount but mostly inaccessible to plants because soluble phosphorus easily precipitates with calcium in alkaline soils and with iron and aluminium in acidic soils. Plants uptake less than 1% of the total phosphorus in the form of orthophosphate anions (mainly as HPO42− and H2PO4−) (Achal et al. 2007; Zhu et al. 2011). Therefore, it is necessary to provide an unavailable form of P to the plant by using different mechanisms. The most feasible approach is to use microbes to solubilize these insolubilized P compounds. The microbes can solubilize phosphorus by secreting phosphatases enzymes or releasing organic acids and protons (Sharma et al. 2020). Plant growth and development are enhanced by the increased availability of phosphorus(Gouda et al. 2016).
The Bacillus and Pseudomonas are the most efficient phosphate-solubilizing microorganisms (El-Deeb et al. 2013). Li et al (2018a, b, c) reported that endophytic bacterial genera such as Bacillus and Microbacterium isolated from medicinal plant Glycyrrhiza uralensis were able to phosphate solubilization. Additionally, the medicinal plant Ferula sinkiangensis had almost 19% of the endophytic bacteria related to phosphate solubilization (Liu et al. 2017). Moreover, many researchers reported that medicinal plants like Vitis vinifera, Capsicum annuum, Trigonella foenum, Trigonella foenum-graecum and Lactuca sativa were associated with endophytic microbes which can solubilize phosphate for the growth and development of medicinal plants (Li et al. 2018a, b, c; Radhakrishnan et al. 2017).
Enhancement of uptake and translocation of micronutrients
Micronutrients play a crucial role in plant metabolism, photosynthesis, respiration and biosynthetic reactions. These are also important constituents of cytochromes, ribosomes and co-factors of different enzymes such as catalase, peroxidase, DNA polymerase and RNApolymerase (Kerkeb and Connoly 2006; Mahender et al. 2019; Rout and Sahoo 2015). Micronutrient use efficiency is only 2–5% (Tian et al. 2008). Rhizospheric or endophytic microorganisms have a significant contribution to the enhancement of uptake and translocation of micronutrients through different mechanisms (Singh et al. 2018; Singh et al. 2017a; Singh et al. 2017b; Singh and Prasanna, 2020) such as: (1) siderophore production; (2) organic acid secretion in root exudates; (3) secretion of phytase enzyme; (4) secretion of metal chelating substances; (5) upregulation of micronutrient transporter or genes; (6) modification of root morphology and anatomy.
Siderophores are iron-binding chelating compounds that are produced by endophytic or rhizospheric microbes to improve plant growth and development by providing iron to plants and by inhibiting the growth of plant pathogenic microorganisms in the root zone or plant biological system (Mohamad et al. 2020; Niessen and Soppa, 2020). Siderophores convert insoluble ferric ions or Fe(OH)3 into the soluble ferric complex compound that can be uptake by plants (Solanki et al. 2014). Li et al. (2018a, b, c) found that around 23 and 57% of the endophytes related to the medicinal plant's Glycyrrhiza uralensis Fisch. and Ferula sinkiangensis secreted siderophores, respectively, and these microbes belongs to different group of microbe genera such as Bacillus, Achromobacter, and Janibacter. The endophytic bacteria associated with medicinal plants such as Capsicum annuum, Launaea nudicaulis, Jatropha curcas, Arachis Hypogaea, Brassica oxyrrhina, and Brassica napus were showed siderophore production ability (Egamberdievaand Tiezzi 2019; Ma et al. 2010; Mohamad et al. 2019; Radhakrishnanand Lee 2016).Besides iron, endophytes also enhance Zn availability to plants through organic acids secretion in root exudates (Singh et al. 2017b).The most effective Zn solubilizing Gram-positive microscopic organisms have to belong to the genus Bacillus. Furthermore, it has been reported that Bacillus amyloliquefasciens, Bacillus megaterium, and Bacillus sp., show phosphorus, potassium, and zinc solubilization (Verma et al. 2015).
Production of phytohormones
Phytohormones are signal molecules that coordinate cellular activities and management of medicinal plant growth, development and vigor. The best-known example of phytohormones that are produced by endophytic microbiota is indole-3-acetic acid (IAA), which is synthesised via the indole-3-pyruvate pathway. Like rhizospheric microbes, endophytic microbes have been shown to have plant growth promoting activities that can be due to the production of phytohormones and enzymes involved in growth regulation, such as ethylene, 1-aminocyclo- propane-1-carboxylic acid (ACC) deaminase, 2,3-butanediol, cytokinins, auxins, indole-3-acetic acid (IAA), acetoin, or combinations of these plant growth hormones (Asaf et al. 2017; Egamberdieva et al. 2017c; Li et al. 2018a, b, c). Indole-3-acetic acid (IAA) is the phytohormone responsible for stimulating cell division, cell elongation, differentiation, and gene regulation to promote plant growth and development (Sharma et al. 2020). Recently, many IAA producing endophytic bacteria (Arthrobacter sp., Enterobacter sp., Pantoea sp., Bacillus sp., Brevibacterium sp., Achromobacter sp., and Stenotrophomonas sp.) have been reported to be associated with the medicinal plants Hypericum perforatum, Ferula sinkiangensis, Ziziphora capitate Ajuga bracteosa (Egamberdieva et al. 2017b; Li et al. 2018a, b, c; Liu et al. 2017; Naragani et al. 2016). The endophytic fungus Fusarium sp. impacts on the growth and development of Euphorbia pekinensis (E5 and E4) by producing IAA and GA (Dai et al. 2003). The fungi Fusarium sp. DL26 and Pyrenochaeta sp. DL351 could improve the growth and development of the orchid Dendrobium loddigesii Rolfe by root elongation by secreting IAA and NH3. (Chen et al. 2010).
El-Deeb et al. (2013) reported that Plectranthus tenuiflorus medicinal plant was inhabitated by Bacillus sp., Bacillus megaterium, Bacillus pumilus, Bacillus licheniformis, Micrococcus luteus, Paenibacillus sp., Pseudomonas sp., and Acinetobacter calcoaceticus endophytes. These endophytic isolates effectively produce gibberellic acid (GA) and cytokinin. GA has biologically important functions such as plant growth-promoting factors, i.e., stem and root elongation, flowering, and/or helping to overcome dormancy in seeds. Cytokinins are a category of plant hormones that play an important role during the cell cycle, i.e., induce the plant cellular division process and, consequently, influence the formation and relative growth of roots and shoots (Arkhipova et al. 2007). Abscisic acid (ABA), which is considered a plant stress hormone, is responsible for many types of stress, including water, salt, and low temperatures (Fahad et al. 2015). Salicylic acid (SA) is also known as a critical phytohormone that is involved in various processes, like seed germination, root initiation, floral induction, and thermogenesis, besides plant tolerance to biotic and abiotic stresses (Yan et al. 2019; Fahad et al. 2015).
Resistance toward abiotic stresses
Plants are exposed to a number of factors which are detrimental to their growth, productivity, and survival in the environment, known as stress. It can be both biotic and abiotic in nature. Abiotic stresses include salinity, pH, temperature fluctuations, excess or low water, irradiance, nutrient deficiency or excess, and even mechanical injury and wounding. Whereas, biotic stress can include various pathogens (bacteria, fungi, and viruses) and herbivores. Endophytic bacteria often produce various compounds that can alleviate these stresses and confer protection to the plant. According to various reports, plants on inoculation with these endophytes often accumulate compounds mainly, carbohydrates, proline, various lytic enzymes, and antibiotics that can inhibit various pathogens (Brader et al. 2014). Moreover, it causes the plant to develop resistance against pathogens by complementing the induced systemic resistance mechanisms (Pieterse et al. 2014).
Endophytes are known to provide various plant-growth promoting nutrients as well as assist in coping with various environmental stresses, which ultimately improves plants yield and productivity. It is therefore of great concern in having knowledge about endophytes, their role in promoting plant growth and their biocontrol, defence and resistance to disease and environment. Acremonium strictum AL16 an endophytic fungus in association with a Chinese medicinal herb, Atractylodes lancea improved the plant growth traits and also alleviated drought conditions by increasing soluble sugars, proteins, proline and antioxidant enzyme activity. It also increased the abscissic acid levels in the host as well as decreased the degree of plasmalemma oxidation, thereby conferring protection to the host plant (Yang et al. 2014).
Unfavourable environmental conditions, viz. temperature extremes, heavy metal toxicity, salinity, drought, and floods can negatively impact plant growth, development and yield (Farooq et al. 2009; Wang et al. 2003; Zhu 2002). Endophytes can help medicinal plants in alleviating these stresses and promoting their growth (Nadeem et al. 2014; Shahzad et al. 2015) For instance, Pseudomonas fluorescence when inoculated in Madagascar periwinkle (Catharanthus roseus) improved its growth attributes as compared to control uninoculated plants under water deficit stress conditions (Jaleel et al. 2007). Furthermore, Pseudomonas fluorescens along with Piriformospora indicain a dual inoculation improved the transplanting shock in musli (Chlorophytum sp.) (Gosal et al. 2010). Also, when an autochthonous isolate was tested along with native mycorrhizal fungi (alone and in consortium), plant growth was stimulated along with improved nutrient and drought tolerance responses (Armada et al. 2016).
Gagné-Bourque et al. (2016) reported the increase of total soluble carbohydrates along with glucose, fructose and starch in drought induced Brachypodium distachyon grass associated with Bacillus subtilis strain B26. However, well-known stress related metabolites (raffinose-related carbohydrate) were not proliferated. Pepper plants (Capsicum annuum L.) in association with Bacillus and Arthrobacter endophytes showed significant proline accumulation in relation to osmotic stress responses (Sziderics et al. 2007). Moreover, endophytes associated with plants can also modulate enzymatic activity causing mitigation of abiotic stress. Damodaran et al. (Damodaran et al. 2014) reported an increase in defence enzymes like Phenylalanine lyase, peroxidase, catalase and superoxide dismutase on inoculation with endophytes like Bacillus subtilus, Bacillus cereus, Bacillus thuringiensis, Bacillus saffensis, Bacillus pumilus, and Bacillus marisflavi in response to high sodium concentrations in gladiolus plants. Association with endophytes have also resulted in plant growth promotion as well as tolerance to low temperature. Bacillus and other derived genus have been associated with Triticum aestivum cultivated in northern hills zone of India, showing its temperature flexibility (Verma et al. 2015).
Resistance toward biotic stresses (biological control of plant pathogens)
Interest in worldwide food production is expanding so as to take care of the teeming millions. However, plant diseases are turning into an obstacle as to how to accomplish these objectives. Symptoms of different plant diseases can range from rusts, blights, scabs, cankers, spots, mildews, wilts and many more as they affect the quality and quantity of the produce. A number of approaches can be followed to control the disease progression, mainly cultural, chemical and biological. Cultural practices are time consuming and does not guarantee complete protection whereas chemical control strategies are not environmentally friendly and cause resistance in pathogens. Biological control, which involves the use of microorganisms to inhibit or reduce disease incidences, provides a practical solution to the problem (Maloy, 1993) (Table 4).
Endophytes show biocontrol activities in two ways- direct or indirect. Direct biocontrol is shown by the endophyte either through production of antagonist metabolites like lytic enzymes (Strobel 2003), antibiotics (Berg and Hallmann 2006), siderophores, and insecticidal compounds (Azevedo et al. 2000; Hallmann et al. 1998) or by competing with pathogen for space and nutrients. Indirect biocontrol is shown by priming the plant defence mechanism through callose deposition, hydrolysing enzymes production or activating the plant defense proteins (PRPs) in a process known as induced systemic resistance (Kloepper and Ryu 2006). Furthermore, endophytes can trigger plant growth through plant growth promotion (PGP) activities and can outcompete cell death caused by pathogen (Berg and Hallmann 2006). Moreover, endophytes associated with medicinal plants have received special interest as they are the producers of important bioactive secondary metabolites. As such plant microbe interaction involving endophytes and medicinal plants have been extensively reviewed (Sekar and Kandavel 2010; Singh 2013). Figure 3 show the mechanisms employed by endophytes for biological control of plant pathogens.
The use of microbial endophytes as biocontrol agents against several phytopathogenic fungi have been advocated by a number of researchers (Egamberdieva et al. 2017b; Erdogan and Benlioglu 2010; Lacava et al. 2007). They have been used on a number of crops like Nicotiana attenuata (Santhanam et al. 2014), Solanum torvum and Solanum melongena (Achari and Ramesh 2014), Solanum trilobatum (Bhuvaneswari et al. 2013) and have shown biological control due to a number of bioactive metabolites. Egamberdieva et al. (2017a) reported antagonistic activity against the phytopathogens Alternaria alternate, Pythium ultimum, Fusarium oxysporum, Fusarium culmorum, Fusarium solani, Botrytis cinerea, Gaeumannomyces graminis because of the endophytes Bacillus, Pseudomonas, Arthrobacter, Stenotrophomonas and Serratia, isolated from medicinal plants Ziziphora capitata and Hypericum perforatum.
Endophytic microorganisms from medicinal plants also demonstrated antimicrobial activity against human pathogens in addition to phytopathogens. In an investigation by Passari et al. (2015) almost half of the endophytes isolated showed antagonistic activity against at least two out of the four pathogens tested, namely: Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Further, El-Deeb et al. (2013) isolated endophytic microbes from different parts of the medicinal plant Plectranthus tenuiflorus which demonstrated antimicrobial activity against at least one out of the six human pathogens like Klebsiella pneumonia, Proteus mirabilis, Escherichia coli, Streptococcus agalactiae, Staphylococcus aureus and Candida albicans.
Mohamad et al. (2018) isolated 114 endophytes from Glycyrrhiza uralensis, wild ethnomedicinal plant in Xinjiang desert, and estimated their antifungal activity against different phytopathogens namely Alternaria solani, Colletotrichum gloeosporioides, Verticillium dahliae, Fusarium oxysporum f. sp., Fulvia fulva, Pestalotiopsis microspora, Fusarium oxysporum f. sp. vasinfectum, Fusarium graminearum and Ceratocystis fimbriata. The results indicated that endophytes belonged to various genera including Bacillus atrophaeus, Bacillus mojavensis, Bacillus halotolerans, Brevibacterium frigoritolerans, and Nocardioides alkalitolerans. They also inhibited various human pathogens like Bacillus cereus, Salmonella enteritidis, Staphylococcus aureus, and Escherichia coli.Endophytic isolates exhibiting antagonism against a variety of fungal phytopathogens in vitro also had the ability to suppress Fusarium oxysporum f. sp. radicis-lycopersici in vivo (Egamberdieva et al. 2017b). Moreover endophytic fungi were also influenced by insect’s attack, so they also produced bioactive insecticidal compounds.
Production of antimicrobial metabolites
Pathogens in nature have acquired resistance to commonly used synthetic chemicals; therefore, the search for alternate antimicrobial compounds is the need of the hour. Endophytes offer these eco-friendly metabolites, mainly amides and amines, which are not only natural but also nontoxic to mammals. These antimicrobial agents are perhaps secondary metabolites produced by the endophytes. It includes polyketides, which are amino acid derived compounds or terpenes and low molecular weight compounds with varied structures (Keller et al. 2005). Synthesis of these polyketides is carried out by large multimodular complexes known as polyketide synthases (PKS) and they are basically carboxylic acids derived via condensation of acetyl coenzyme A (acetyl CoA) and malonyl CoA forming carbon chains of β-ketone groups (Keller et al. 2005).
Pseudomonas fluorescens Q2-87 an endophytic bacteria whose PKSs synthesise 2,4-diacetylphloroglucinol and has been antagonistic to a number of phytopathogens (Alvin et al. 2014), whereas PKS from Aspergillus nidulans produces the cholesterol lowering compound, lovastatin (Keller et al. 2005). Moreover, along with PKSs, endophytes also synthesize non-ribosomal peptides produced in non-ribosomal peptide synthetases (NRPS), which aids in the condensation of non-proteinogenic and proteinogenic amino acids. Penicillin and cephalosporin, β-lactam antibiotics, are produced by the first identified fungal NRPS (Keller et al. 2005).
Many investigators reported various NRPs namely spoxazomicins, siderophores, and serobactin produced from endophytic microbes of orchids and grasses namely, Streptosporangium oxazolinicum, Pseudomonas aeruginosa, and Herbaspirillum seropedicae respectively (Inahashi et al. 2011; Miller et al. 2012; Rosconi et al. 2013). Moreover, the presence of genes related to NRPS or PKS should be a primary step in characterization of endophytes producing bioactive metabolites as they contain conserved regions critical for their functioning (Miller et al. 2012). The β-ketoacyl synthase (KS) domain of PKS and adenylation (A) domain of NRPS are conserved, which promotes condensation of acetyl CoA and malonyl CoA and which identifies growing peptide chain respectively (Keller et al. 2005).
Chowdhary and Sharma (2020) reported that endophytic fungi (Chaetomium globosum and Trichoderma harzanium) of Aloe vera showed antagonistic activity against Sclerotinia sclerotiorum by the production of various antifungal compounds like hexadecanoic acid, 2, 3-bis[(trimethylsilyl) oxy] propyl ester and other unsaturated fatty acids (palmitic and linoleic acid). A variety of other metabolites like fatty acids, fatty acid methyl ester, alkanes and hydrocarbons have also been reported to suppress fungal pathogens like Fusarium (Al-Rashdi et al. 2020). Morphological changes have been elicited because of these metabolites like loss of turgidity, disintegration and shrinkage of hyphae in the fungal pathogen. Bacillus and Streptomyces species constitutes the most diverse and cosmopolitan gram positive bacterial endophytes present, producing secondary metabolites having antimicrobial property against phytopathogens (Reinhold-Hurek and Hurek, 2011; Frank et al. 2017).
Streptomyces sp. is source of a number of antibiotics, hence is known for its antagonistic activity against different phytopathogens. Streptomyces sp. strain NRRL 30,562 associated with Kennedia nigriscans was reported to be antagonistic against various plant pathogenic bacteria and fungi due to the presence of antibiotics, namely munumbicins A, B, C, and D (Castillo et al. 2006). Also Streptomyces caeruleatus endophyte in leguminous plants was effective against Xanthomonas campestris pv glycine (Mingma et al. 2014). Moreover, endophytic actinobacteria from Azadirachta indica and Nothofagus sp. have been evaluated for their antimicrobial activities against various phytopathogenic fungi such as- Sclerotinia sclerotiorum, Mycosphaerella fijiensis, Pythium, Phytophthora sp. and Rhizoctonia solani (Castillo et al. 2007; Verma et al. 2009). Streptomyces seoulensis characterized from two different plant species in southern Patagonia by Castillo et al. (2007) suggested the plants survival strategy against phytopathogens in that particular area due to production of metabolites. Matsumoto and Takahashi (2017) reported the antagonistic activity of endophytic actinobacteria against Xanthomonas campestris pv. Oryzae strain KB-88, Kocuria rhizophila strain KB-212, and Mucor racemosus strain KF-223.
The genus Bacillus has been reported in several instances to have antimicrobial activity. More often, they have been proposed for crop management (Aloo et al. 2018). Gond et al. (2015) described lipopeptides producing Bacillus subtilis and Bacillus amyloliquefaciens as endophytes in maize seeds and inhibiting Fusarium moniliforme. Similarly, Bacillus amyloliquefaciens endophytic in Bruguiera gymnorrhiza (L.) showed antagonism against a variety of fungal and bacterial pathogens particularly Capsicum wilt in pots as well as field trials (Hu et al. 2010). Moreover, Bacillus cereus and Bacillus mojavensis endophytes in rice exhibited antagonism against a variety of fungal pathogens in rice, namely, Magnaporthe grisea, Magnaporthe salvinii, Fusarium verticillioides, Fusarium fujikuroi, and Fusarium proliferum (Etesami and Alikhani, 2017).
Endophytes isolated from radish mainly Brachybacterium, Paenibacillus and Bacillus subtilis possessed antifungal activity against Rhizoctonia solani, Pythium ultimum, Fusarium oxysporium and Phytophthora capsici(Seo et al. 2010). Furthermore, B. subtilis strain EDR4 endophyte in wheat reported an antifungal protein which inhibited growth of Fusariumgraminearum, Gaeumannomyces graminis var triticiBacilluscinerea, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, and Macrophoma kuwatsukai (Liu et al. 2010a, b).
Egamberdieva et al. (2017a) reported endophytic bacteria of various genera, namely, Bacillus, Arthrobacter, Stenotrophomonas, Erwinia, Serratia, Achromobacter, Pseudomonas, Enterobacter, and Pantoea from Ziziphora capitata and Hypericum perforatum. Latter supported the growth of various bacteria having antagonistic activity against F. oxysporum than the former. On the contrary, Fusarium oxysporum as an endophyte can produce antagonistic compounds against nematode such as gibepyrone D, 4-hydroxybenzoic acid and indole-3-acetic acid (IAA) (Bogner et al. 2017).
Another significant class of antimicrobial secondary metabolites are the lipopeptides, which are known for their antimicrobial activity as well as activator of plant defence machinery (Stein, 2005; Raaijmakers et al. 2010). There are several isoforms of polypeptides which can be produced by the same bacterial species. The most investigated ones are related to Bacillus and Paenibacillus lipopeptides (Villarreal-Delgado et al. 2018). Moreover, many Bacillus amyloliquefaciens strains are known to produce higher lipopeptides (Ongena and Jacques, 2008).
Production of hydrolytic enzymes
The antagonistic activity of endophytes against various pathogens can also be due to their ability to produce a number of lytic enzymes. These enzymes can degrade the cell wall polymer complexes of different pathogens and make it susceptible to lysis. Lipases and chitinases are enzymes which degrade the chitinous cell wall of fungus making it a potent biocontrol agent against phytopathogens (Suresh et al. 2010; Wahyudi et al. 2011). Pseudomonas sp. endophytically associated with a number of medicinal plants like Coleus forskohlii, Launaea nudicaulis, and Cupressus sempervirens produced chitinolytic enzymes and exhibited control against phytopathogens, namely, Fusarium chlamydosporum, Fusarium solani, Fusarium oxysporum, Seiridium cardinal, Ralstonia solanacearum, and Macrophomina phaseolina (Mansoor et al. 2007; Raio et al. 2011; Singh et al. 2013). Furthermore, Bacillus sp. as endophyte in association with some medicinal plants like Glycyrrhiza uralensi, Panax quinquefolius, and Arachis hypogaea controlled plant diseases because of their ability to produce chitinolytic enzymes (Mohamad et al. 2018; Nautiyal et al. 2013; Song et al. 2014). Sahu et al. (2020) reported different strains of Bacilli isolated from Ocimum tenuiflorum showing antagonism against sheath blight of rice by inducing defense enzymes mainly phenyl ammonia lyase, peroxidase, and polyphenol oxidase. In similar study endophytes in association with Ziziphora capitata, a medicinal plant, also produced chitinolytic enzymes (Egamberdieva et al. 2017a). Moreover, different lytic enzymes like cellulases, lipases and proteases were produced by endophytes associated with plants like Ferula sinkiangensis, Ferula songorica, Glycyrrhiza uralensis, and Hypericum perforatum (Egamberdieva et al. 2017a; Li et al. 2018a, b, c; Liu et al. 2017; Liu et al. 2016a, b).
Disrupting quorum sensing signals in pathogens
Pathogens communicate with each other via signalling molecules such as N-acyl homoserine lactones (AHLs), often called as quorum sensing (Waters and Bassler 2005). This cell to cell communication is responsible for regulating the expression of various virulence factors contributing in the pathogenicity (Fuqua et al. 2001). Endophytes can degrade these QS signals, called as Quorum quenching, which can ultimately reduce the virulence of the pathogen (Helman and Chemin, 2015). For example, many Bacillus species can produce AHL lactonases which can degrade these signalling molecules, thereby diminishing the virulence (Zhou et al. 2008). Rajesh and Rai (2014) investigated the potential of endophytic Bacillus firmus and Enterobacter asburiae associated with Pterocarpus santalinus in degrading the AHLs and preventing Pseudomonas aeruginosa biofilm formation. Moreover, these enzymes have also been reported in various other endophytic microbes like Pseudomonas, thus, contributing in better disease control and improved plant health.
Inducing overproduction of antimicrobial or immunological compounds by plants
Notwithstanding the previously mentioned approaches, endophytic microbes can also stimulate the immunological or antimicrobial responses in plants through the production of varied compounds. In a study done by Siddaiah et al. (2017),Trichoderma hamatum UoM 13, an endophytic fungus can lead to the overproduction of various defence enzymes, salicyclic acid and pathogenesis related proteins (PRP) which complements systemic immunity against downy mildew in pearl millet. Moreover, there has been an upregulation of host defence genes (Tc00g04254) in Theobroma cacao leaves because of endophtic fungus Colletotrichum tropicale on pathogen damage (Mejía et al. 2014). Kushwaha et al. (2019) studied the compatibility of fungal endophytes (Aspergillus, Sarocladium and Penicillium) of Withania somnifera with biological control (Trichoderma viridae) and reported that the co-inoculation significantly increased the withanolide A content by expressing its biosynthetic genes.
Space competition with pathogens
Endophytes can often compete with the pathogen for space, thereby limiting its ability to grow and cause disease. Fungal endophytes, in particular, proliferate rapidly, thereby exhausting the nutrients for the pathogen to grow. It has been suggested that colonization by Beauveria bassiana endophytically can control the damping off and root rot of tomato and cotton, competition for space being the likely mechanism for biological control against Rhizoctonia solani and other pathogens (Ownley et al. 2008). Moreover, in response to endophyte, the plant produces lignin and other cell wall components, thereby limiting the growth of the endophyte and maintaining its avirulence (Harman et al. 2004).
Conclusions and future prospects
For medicinal and aromatic plants, endophytes have more valuable functions, such as controlling phytopathogens, promoting plant growth and modulating the phyto-chemistry. Endophytes of medicinal and aromatic plants are more efficient in producing bioactive secondary metabolites that can be used as antimicrobial agents against human, animal, and plant pathogens. Application of endophytes as a substitute for pesticides or chemical fertilisers is more fascinating because it prompts use of medicinal plants by the consumer and the pharmacology industry without any residue of synthetic chemicals. Thus, endophytes may be more prominent agents in scavenging synthetic chemical compounds and enhancing the production of bioactive secondary metabolites in medicinal plants. Therefore, there is a further need to isolate endophytes from medicinal plants to explore as a plant growth promoting agent and detect the bioactive compounds to utilise instead of synthetic chemicals to save the environment. Endophytes of medicinal and aromatic plants could produce some important bioactive secondary metabolites freely, which enables the pharmacological industry to conduct large-scale fermentation of bioactive secondary metabolites, independent of the cultivation of medicinal plants.
Data availability
Data sharing not applicable to this article as no datasets were generated or analysed during the current study.
Code availability
Not applicable.
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Singh, D., Thapa, S., Mahawar, H. et al. Prospecting potential of endophytes for modulation of biosynthesis of therapeutic bioactive secondary metabolites and plant growth promotion of medicinal and aromatic plants. Antonie van Leeuwenhoek 115, 699–730 (2022). https://doi.org/10.1007/s10482-022-01736-6
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DOI: https://doi.org/10.1007/s10482-022-01736-6