Overview
- Editors:
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Stanton B. Gelvin
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Purdue University, West Lafayette, USA
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Robbert A. Schilperoort
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Leiden State Universiy, Leiden, The Netherlands
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Desh Pal S. Verma
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Ohio State University, Columbus, USA
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About this book
During the past ten years, great advances have been made in the area of plant molecular biology. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. Additional chapters that can replace or be added to this first edition will be published periodically. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Those people desiring such material should contact the relevant authors directly. A list of the various contributors to this manual, including their addresses, is included.
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Table of contents (24 chapters)
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Front Matter
Pages I-VIII
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Introduction of DNA into Plant Cells
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- Michael W. Saul, Raymond D. Shillito, Ioan Negrutiu
Pages 1-16
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- Stephen G. Rogers, Harry Klee, Robert B. Horsch, Robert T. Fraley
Pages 17-25
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- Gynheung An, Paul R. Ebert, Amitava Mitra, Sam B. Ha
Pages 29-47
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- Robert B. Horsch, Joyce Fry, Nancy Hoffmann, Jeanne Neidermeyer, Stephen G. Rogers, Robert T. Fraley
Pages 63-71
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- Scott O. Rogers, Arnold J. Bendich
Pages 73-83
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- Jerry L. Slightom, Hector D. Quemada
Pages 85-136
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- Jerry L. Slightom, Roger F. Drong
Pages 137-178
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- Arlette Reynaerts, Marc De Block, Jean-Pierre Hernalsteens, Marc Van Montagu
Pages 179-194
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- Robert C. Garber, B. Gillian Turgeon, Olen C. Yoder
Pages 195-207
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Expression of Genes in Plants
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- Luis Herrera-Estrella, Teemu H. Teeri, June Simpson
Pages 209-230
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- Anna-Lisa Paul, Robert J. Ferl
Pages 231-241
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- Ferenc Nagy, Steve A. Kay, Nam-Hai Chua
Pages 271-299
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- P. H. Schreier, B. Reiss, M. Kuntz
Pages 301-322
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- Sacco De Vries, Harry Hoge, Ton Bisseling
Pages 323-335
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- E. E. Kawata, G. Galili, L. D. Smith, B. A. Larkins
Pages 337-358
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- Stephen H. Howell, David W. Ow, Michel Schneider
Pages 359-369
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- Natasha V. Raikhel, Sebastian Y. Bednarek, David R. Lerner
Pages 371-402
Editors and Affiliations
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Purdue University, West Lafayette, USA
Stanton B. Gelvin
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Leiden State Universiy, Leiden, The Netherlands
Robbert A. Schilperoort
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Ohio State University, Columbus, USA
Desh Pal S. Verma