Abstract
The development of several in vitro transcription systems based on phage-specific RNA polymerases has made it possible to synthesize large quantities of RNAs from cloned DNA sequences. These transcripts can be translated by in vitro translation systems or introduced into Xenopus laevis oocytes to study post-transcriptional, translational, or post-translation al processes. Here we describe one of the in vitro transcription systems that utilizes a specific promoter and the RNA polymerase from the phage SP6 of Salmonella typhimurium. We also describe methods used to analyze translation of these RNAs in the Xenopus oocyte.
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© 1989 Kluwer Academic Publishers, Dordrecht
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Kawata, E.E., Galili, G., Smith, L.D., Larkins, B.A. (1989). Translation in Xenopus oocytes of mRNAs transcribed in vitro . In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_17
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DOI: https://doi.org/10.1007/978-94-009-0951-9_17
Publisher Name: Springer, Dordrecht
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