Abstract
Many of the proteins in chloroplasts are encoded by the nuclear genome. The most extensively studied of these is the small subunit of ribulose-l,5-bisphosphate carboxylase/oxygenase that is encoded by a small gene family. The translation product of cytoplasmic ribosomes is a precursor protein with an NH2-terminal extension. This precursor is transported into the chloroplasts post-translationally in an energy-dependent process (for reviews, see [4, 25]). During or shortly after translocation the transit peptide is cleaved off to yield the mature small subunit. In vitro experiments indicate that the maturation occurs via a processing intermediate [22]. The mature polypeptide is directed to the stroma where it assembles with the chloroplast-encoded large subunit to form a functional holoenzyme.
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Abbreviations
- APS:
-
ammoniumperoxodisulfate
- TEMED:
-
N,N,N’, N’-tetramethylethylene-diamine
- TPCK:
-
L-l-tosylamide-2-phenylethylchlormethylketone
- BSA:
-
bovine serum albumin
- DEPC:
-
diethyl-pyrocarbonate
- DTT:
-
dithiothreitol
- EtBr:
-
ethidium bromide
- PEG:
-
polyethyleneglycol
- RNasin:
-
placental RNase inhibitor
- SAM:
-
S-adenosyl-methionine
- TCA:
-
trichloracetic acid
- PMSF:
-
phenylmethylsulfonyl fluoride
- BAM:
-
benzamidine
- ACA:
-
E-amino-n-caproic acid
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© 1989 Kluwer Academic Publishers, Dordrecht
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Schreier, P.H., Reiss, B., Kuntz, M. (1989). Subcellular targeting of proteins in vivo and in vitro . In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_15
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DOI: https://doi.org/10.1007/978-94-009-0951-9_15
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