Abstract
The transfer of foreign DNA to plant cells can be achieved in a variety of ways that are described in several chapters of this manual. This chapter describes the various selectable and screenable marker genes that are presently used in plant transformation experiments. Screenable markers have been widely used in gene constructs to study the regulation of plant gene expression.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79:568–570.
Blochlinger K, Diggelman H (1984) Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol Cell Biol (1984): 2929–2931.
Budar F, De Boeck F, Van Montagu M, Hernalsteens JP (1987) Introduction and expression of the octopine T-DNA oncogenes in tobacco plants and their progeny. Plants Sci (in press).
De Block M, Herrera-Estrella L, Van Montagu M, Schell J, Zambryski P (1984) Expression of foreign genes in regenerated plants and in their progeny. EMBO J 3:1681–1689.
Ellis JG, Ryder MH, Tate ME (1984) Agrobacterium tumefaciens TR-DNA encodes a pathway for agropine biosynthesis. Mol Gen Genet 195:466–473.
Herrera-Estrella L, De Block M, Messens E, Hernalsteens JP, Van Montagu M, Schell J (1983) Chimeric genes as dominant selectable markers in plant cells. EMBO J 2:987–995.
Herrera-Estrella L, Van den Broeck G, Maenhout R, Van Montagu M, Schell J, Timko M, Cashmore A (1984) Light inducible and chloroplast associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature 310:115–120.
Horsch RB, Fry JE, Hoffmann NL, Wallroth M, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231.
Joos H, Inzé D, Caplan A, Sormann M, Van Montagu M (1983) Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell 32:1057–1067.
Jorgensen R (1986) Introduction of improved marker for male sterile genes via Ti. Proceedings of the Tomato Biotechnology Symposium (University of California, Davis, August 1986).
Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) ‘In vitro’ transformation of plant protoplasts with Ti plasmid DNA. Nature 296:72–74.
Leemans J, De Blaere R, Willmitzer L, De Greve H, Hernalsteens JP, Van Montagu M, Schell J (1982) Genetic identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J 1:147–152.
Marton L, Wullems GJ, Molendijk L, Schilperoort RA (1979) ‘In vitro’ transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277:129–130.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–497.
Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3:2717–2722.
Reiss B, Sprengel R, Will H, Schaller H (1984) A new sensitive method for quantitative and qualitative assay of neomycine phosphotransferase in crude cell extract. Gene 30:211–218.
Salomon F, De Blaere R, Leemans J, Hernalsteens JP, Van Montagu M, Schell J (1984) Genetic identification of functions of TR-DNA transcripts in octopine crown gall. EMBO J 3:141–146.
Simpson J, Timko MP, Cashmore AR, Schell J, Van Montagu M, Herrera-Estrella L (1985) Light inducible and tissue-specific expression of a chimeric gene under control of a 5′-flanking sequence of a pea chlorophyll a/b-binding protein gene. EMBO J 4:2723–2729.
Somers DA, Gengenbach BG, Biesboer DD, Hackett WP, Green CE (1986) Vlth International Congress of Plant Tissue and Cell Culture (University of Minnesota, Minneapolis, Minnesota, USA), abstracts.
Veiten J, Veiten L, Hain R, Schell J (1984) Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens. EMBO J 3:2723–2730.
Van Den Elzen PJM, Towsend J, Lee KY, Bedbrook JR (1985) A chimaeric hygromycin resistance gene as a selectable marker in plant cells. Plant Mol Biol 5:299–302.
References of the addendum
An G, Watson BD, Chiang CC (1986) Transformation of tobacco, tomato, potato and Arabidopsis thaliana using a binary Ti vector system. Plant Physiol 81:301–305.
Deak M, Kiss GB, Koncz C, Dudits D (1986) Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep 5:97–100.
Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Gallupi GR, Goldberg SB, Hoffmann NL, Woo SC (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80:4803–4807.
Garcia J, Hille J, Goldbach R: Transformation of Vigna unguiculata using Agrobacterium tumefaciens. EMBO Workshop Plant DNA Infectious Agents.
Lloyd AM, Barnason AR, Rogers SE, Byrne MC, Fraley RT, Horsch RB (1986) Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens. Science 234:464–466.
Lorz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol Gen Genet 199:178–182.
Matzke MA, Susani M, Binns AN, Levis ED, Rubenstein I, Matzke AJM (1984) Transcription of a zein gene introduced into sunflower using a Ti plasmid vector. EMBO J (1984): 1525–1531.
Paszkowski J, Pisan B, Shillito RD, Hohn T, Hohn B, Potrykus I (1986) Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome. Plant Mol Biol 6:303–312.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers, Dordrecht
About this chapter
Cite this chapter
Reynaerts, A., De Block, M., Hernalsteens, JP., Van Montagu, M. (1989). Selectable and screenable markers. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_9
Download citation
DOI: https://doi.org/10.1007/978-94-009-0951-9_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6918-2
Online ISBN: 978-94-009-0951-9
eBook Packages: Springer Book Archive