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Multienzymatic Assemblies

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Haralambos Stamatis

    1. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2487)

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About this book

This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes

    • James Christenson
    Pages 1-13

  3. Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells

    • Songon An, Prakash Parajuli, Erin L. Kennedy, Minjoung Kyoung
    Pages 15-26

  4. Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades

    • Svyatoslav Kondrat, Eric von Lieres
    Pages 27-50

  5. Structural Characterization of Multienzyme Assemblies: An Overview

    • Anastassios C. Papageorgiou
    Pages 51-72

  6. Assessing Protein Interactions for Clustering of Mitochondrial Urea Cycle Enzymes

    • Ljubica Caldovic, Shivaprasad Bhuvanendran, Jyoti Jaiswal
    Pages 73-92

  7. DNA Nanoscaffolds for Multienzyme Systems Assembly

    • Sung Won Oh, Zhicheng Wang, Jinglin Fu
    Pages 93-112

  8. Strategies for Multienzyme Assemblies

    • Qing Sun, Maryam Raeeszadeh-Sarmazdeh, Shen-Long Tsai, Wilfred Chen
    Pages 113-131

  9. Random and Positional Immobilization of Multi-enzyme Systems

    • Hassan Maleki, Kamyar Khoshnevisan, Hadi Baharifar
    Pages 133-150

  10. Compartmentalized Immobilization of Multi-enzyme Systems

    • Hadi Baharifar, Kamyar Khoshnevisan, Hassan Maleki
    Pages 151-162

  11. 3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization

    • Elena Gkantzou, Anastasia Skonta, Andreas-Georgios Vasios, Haralambos Stamatis
    Pages 163-175

  12. Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing the Cucurbit[8]Uril Macrocyclic Host

    • Elisavet Ioannou, Nikolaos E. Labrou
    Pages 177-187

  13. Lectin-Mediated Coimmobilization of Cascade Glycoenzymes

    • Yifei Zhang, Yunzhenshan Gao, You Yong
    Pages 189-195

  14. Self-Assembled Multienzyme Nanostructures for Biocatalysis in Cellulo

    • Qixin Wei, Jiang Xia
    Pages 197-204

  15. Nanoarmored Multi-Enzyme Cascade Catalysis

    • Mansi Malhotra, Ankarao Kalluri, Challa Vijaya Kumar
    Pages 205-225

  16. Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds

    • Joyce C. Breger, Gregory A. Ellis, Scott A. Walper, Kimihiro Susumu, Igor L. Medintz
    Pages 227-262

  17. A Four-enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-step Cascade Reactions

    • Archontoula Giannakopoulou, Michaela Patila, Nikolaos Chalmpes, Angeliki C. Polydera, Dimitrios Gournis, Haralambos Stamatis
    Pages 263-278

  18. A Bi-enzymatic Immobilized Nanobiocatalyst for the Biotransformation of Oleuropein to Hydroxytyrosol

    • Archontoula Giannakopoulou, Alexandra V. Chatzikonstantinou, Aliki Tsakni, Christos L. Chochos, Dimitra Houhoula, Haralambos Stamatis
    Pages 279-296

  19. A Simple Recombinant E. coli Cell Lysate-Based Biocatalyst for ATP-Dependent Multi-step Reactions

    • Natalia Bartzoka, Thomas D. Loan, Hideki Onagi, Apostolos Alissandratos
    Pages 297-315

  20. CO2 to Methanol: A Highly Efficient Enzyme Cascade

    • Io Antonopoulou, Ulrika Rova, Paul Christakopoulos
    Pages 317-344
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Editors and Affiliations

  • Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Haralambos Stamatis

Bibliographic Information

  • Book Title: Multienzymatic Assemblies

  • Book Subtitle: Methods and Protocols

  • Editors: Haralambos Stamatis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2269-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2268-1Published: 11 June 2022

  • Softcover ISBN: 978-1-0716-2271-1Published: 11 June 2023

  • eBook ISBN: 978-1-0716-2269-8Published: 10 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 379

  • Number of Illustrations: 12 b/w illustrations, 77 illustrations in colour

  • Topics: Enzymology, Chemistry/Food Science, general

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