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Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing the Cucurbit[8]Uril Macrocyclic Host

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Multienzymatic Assemblies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2487))

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Abstract

Self-assembly is a phenomenon that governs molecular structural organization in nature, therefore raising research interest for the fabrication of novel nanomaterials with diverse applications in biocatalysis, biomedicine, material templating, and biosensor development. In this chapter we provide protocols for the development of supramolecular protein complexes based on host–guest interactions in the presence of the macrocyclic host, cucurbit[8]uril (CB[8]). CB[8] is reported to exhibit high binding affinity towards the tripeptide Phe-Gly-Gly (FGG), therefore it can be utilized as a selective adhesive of protein molecules, after fusion of FGG to an accessible protein surface.

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Acknowledgements

This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ).

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Authors and Affiliations

  1. Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

    Elisavet Ioannou & Nikolaos E. Labrou

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  1. Elisavet Ioannou
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  2. Nikolaos E. Labrou
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Correspondence to Nikolaos E. Labrou .

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Editors and Affiliations

  1. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Haralambos Stamatis

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Ioannou, E., Labrou, N.E. (2022). Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing the Cucurbit[8]Uril Macrocyclic Host. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2269-8_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2268-1

  • Online ISBN: 978-1-0716-2269-8

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