Abstract
3D printing is lately utilized in biological sciences under the scope to develop customized scaffolds that will host biomolecules, either whole cells or parts of them, like enzymes. In the present work, we present a protocol to modify the surface of 3D printed polylactic acid (PLA) well-plates with the aim to co-immobilize multiple enzymes that will perform cascade reactions. Detailed steps to design and print the final models are described. The developed protocol for surface modification is based on coating with chitosan biopolymer and covalent immobilization of the enzymes β-glucosidase, glucose oxidase, and peroxidase via glutaraldehyde cross-linking. Enzymatic activity measurements indicative of the catalytic performance of the system are also presented.
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Acknowledgments
This research has been co-financed by the European Regional Development Fund of the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship, and Innovation, under the call RESEARCH− CREATE−INNOVATE (project code: Τ2ΕDΚ- 01410). E.G. is thankful for the financial support to the Operational Program ≪Human Resources Development, Education and Lifelong Learning≫ in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ), which is co-financed by Greece and the European Union (European Social Fund-ESF).
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Gkantzou, E., Skonta, A., Vasios, AG., Stamatis, H. (2022). 3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_10
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DOI: https://doi.org/10.1007/978-1-0716-2269-8_10
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