Abstract
With the discovery of targeted gene replacement, moss biology has been rapidly advancing over the last 10 years. This study demonstrates the usefulness of moss as a model organism for plant photosynthesis research. The two mosses examined in this study, Physcomitrella patens and Ceratodon purpureus, are easily cultured through vegetative propagation. Growth tests were conducted to determine carbon sources suitable for maintaining heterotrophic growth while photosynthesis was blocked. Photosynthetic parameters examined in these plants indicated that the photosynthetic activity of Ceratodon and Physcomitrella is more similar to vascular plants than cyanobacteria or green algae. Ceratodon plants grown heterotrophically appeared etiolated in that the plants were taller and plastids did not differentiate thylakoid membranes. After returning to the light, the plants developed green, photosynthetically active chloroplasts. Furthermore, UV-induced mutagenesis was used to show that photosynthesis-deficient mutant Ceratodon plants could be obtained. After screening approximately 1000 plants, we obtained a number of mutants, which could be arranged into the following categories: high fluorescence, low fluorescence, fast and slow fluorescence quenching, and fast and slow greening. Our results indicate that in vivo biophysical analysis of photosynthetic activity in the mosses can be carried out which makes both mosses useful for photosynthesis studies, and Ceratodon best sustains perturbations in photosynthetic activity.
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Abbreviations
- CCD:
-
Charge-coupled device
- DCMU:
-
dichlorophenyl-dimethylurea
- PSII:
-
photosystem II
- EST:
-
expressed sequence tag
References
NW Ashton DJ Cove (1977) ArticleTitleThe isolation and preliminary characterization of auxotrophic analogue resistant mutants in the moss Physcomitrella patens Mol Gen Genet 154 87–95 Occurrence Handle10.1007/BF00265581
M Bezanilla A Pan RS Quatrano (2003) ArticleTitleRNA interference in the moss Physcomitrella patens Plant Physiol 133 470–474 Occurrence Handle10.1104/pp.103.024901 Occurrence Handle14555775
TM Bricker J Lowrance H Sutton LK Frankel (2001) ArticleTitleAlterations of the oxygen-evolving apparatus in a (448)Arg– (448)S mutant in the CP47 protein of photosystem II under normal and low chloride conditions Biochemistry 40 11483–11489 Occurrence Handle10.1021/bi010906x Occurrence Handle11560496
DJ Cove W Kammerer CD Knight MJ Leech CR Martin TL Wang (1991) ArticleTitleDevelopmental genetic studies of the moss, Physcomitrella patens Symp Soc Exp Biol 45 31–43 Occurrence Handle1843414
D Cove C Knight T Lamparter (1997) ArticleTitleMosses as model systems Trends Plant Sci 2 99–105 Occurrence Handle10.1016/S1360-1385(96)10056-X
DH Engels A Lott GH Schmid EK Pistorius (1994) ArticleTitleInactivation of the water-oxidizing enzyme in manganese stabilizing protein-free mutant cells of the cyanobacteria Synechococcus PCC7942 and Synechocystis PCC6803 during dark incubation and conditions leading to photoactivation Photosynth Res 42 227–244 Occurrence Handle10.1007/BF00018265
S Frances MJ White MD Edgerton AM Jones RC Elliott WF Thompson (1992) ArticleTitleInitial characterization of a pea mutant with light-independent photomorphogenesis Plant Cell 4 1519–1530 Occurrence Handle10.1105/tpc.4.12.1519 Occurrence Handle1467651
PA Girod H Fu JP Zryd RD Vierstra (1999) ArticleTitleMultiubiquitin chain binding subunit MCB1 (RPN10) of the 26S proteasome is essential for developmental progression in Physcomitrella patens Plant Cell 11 1457–1472 Occurrence Handle10.1105/tpc.11.8.1457 Occurrence Handle10449580
NH Grimsley NW Ashton DJ Cove (1977) ArticleTitleThe production of somatic hybrids by protoplast fusion in the moss Physcomitrella patens Mol Gen Genet 154 97–100 Occurrence Handle10.1007/BF00265582
Y Hiwatashi T Nishiyama T Fujita M Hasebe (2001) ArticleTitleEstablishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens Plant J 28 105–116 Occurrence Handle10.1046/j.1365-313X.2001.01121.x Occurrence Handle11696191
T Imaizumi A Kadota M Hasebe M Wada (2002) ArticleTitleCryptochrome light signals control development to suppress auxin sensitivity in the moss Physcomitrella patens Plant Cell 14 373–386 Occurrence Handle11884681
T Kaneko S Sato H Kotani A Tanaka E Asamizu Y Nakamura N Miyajima M Hirosawa M Sugiura S Sasamoto T Kimura T Hosouchi A Matsuno A Muraki N Nakazaki K Naruo S Okumura S Shimpo C Takeuchi T Wada A Watanabe M Yamada M Yasuda S Tabata (1996) ArticleTitleSequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions DNA Res 3 109–136 Occurrence Handle8905231
N Keren I Ohad (1998) State transisitions and photoinhibition J-D Rochaix M Goldschmidt-Clermont S Merchant (Eds) The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas, pp 569–596 Kluwer Academic Publishers Dordrecht, The Netherlands
A Koprivova AJ Meyer G Schween C Herschbach R Reski S Kopriva (2002) ArticleTitleFunctional knockout of the adenosine 5’-phosphosulfate reductase gene in Physcomitrella patens revives an old route of sulfate assimilation J Biol Chem 277 32195–32201 Occurrence Handle10.1074/jbc.M204971200 Occurrence Handle12070175
JH Luft (1961) ArticleTitleImprovements in epoxy resin embedding methods J Biophys Biochem Cytol 9 409–414 Occurrence Handle13764136
P Meunier (1993) ArticleTitleOxygen evolution by photosystem II: the contribution of backward transitions to the anomalous behavior of double-hits revealed by a new analysis method Photosynth Res 36 111–118 Occurrence Handle10.1007/BF00016276
J Meurer H Plucken KV Kowallik P Westhoff (1998) ArticleTitleA nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana EMBO J 17 5286–5297 Occurrence Handle10.1093/emboj/17.18.5286 Occurrence Handle9736608
L Nedbal M Trtilek D Kaftan (1999) ArticleTitleFlash fluorescence induction: a novel method to study regulation of photosystem II J Photochem Photobiol B Biol 48 154–157 Occurrence Handle10.1016/S1011-1344(99)00032-9
I Ohad P Siekevitz GE Palade (1967) ArticleTitleBiogenesis of chloroplast membranes II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardii) J Cell Biol 35 553–584 Occurrence Handle10.1083/jcb.35.3.553 Occurrence Handle6064365
P Pesaresi C Varotto E Richly A Lessnick F Salamini D Leister (2003) ArticleTitleProtein–protein and protein–function relationships in Arabidopsis photosystem I: cluster analysis of PS I polypeptide levels and photosynthetic parameters in PS I mutants J Plant Physiol 160 17–22 Occurrence Handle10.1078/0176-1617-00920 Occurrence Handle12685041
S Pursiheimo E Rintamaki E Baena-Gonzalez EM Aro (1998) ArticleTitleThylakoid protein phosphorylation in evolutionally divergent species with oxygenic photosynthesis FEBS Lett 423 178–182 Occurrence Handle10.1016/S0014-5793(98)00088-X Occurrence Handle9512353
J Raymond O Zhaxybayeva JP Gogarten SY Gerdes RE Blankenship (2002) ArticleTitleWhole-genome analysis of photosynthetic prokaryotes Science 298 1616–1620 Occurrence Handle10.1126/science.1075558 Occurrence Handle12446909
D Schaefer JP Zryd CD Knight DJ Cove (1991) ArticleTitleStable transformation of the moss Physcomitrella patens Mol Gen Genet 226 418–424 Occurrence Handle2038304
J Schwuchow FD Sack E Hartmann (1990) ArticleTitleMicrotubule distribution in gravitropic protonemata of the moss Ceratodon Protoplasma 159 60–69 Occurrence Handle10.1007/BF01326635 Occurrence Handle11537091
R Strepp S Scholz S Kruse V Speth R Reski (1998) ArticleTitlePlant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin Proc Natl Acad Sci USA 95 4368–4373 Occurrence Handle10.1073/pnas.95.8.4368 Occurrence Handle9539743
E Turcsanyi I Vass (2000) ArticleTitleInhibition of photosynthetic electron transport by UV-A radiation targets the photosystem II complex Photochem Photobiol 72 513–520 Occurrence Handle10.1562/0031-8655(2000)072<0513:IOPETB>2.0.CO;2 Occurrence Handle11045723
LM Walker FD Sack (1990) ArticleTitleAmyloplasts as possible statoliths in gravitropic protonemata of the moss Ceratodon purpureus Planta 181 71–77 Occurrence Handle10.1007/BF00202326 Occurrence Handle11538268
RG Walters F Shephard JJ Rogers SA Rolfe P Horton (2003) ArticleTitleIdentification of mutants of Arabidopsis defective in acclimation of photosynthesis to the light environment Plant Physiol 131 472–481 Occurrence Handle10.1104/pp.015479 Occurrence Handle12586872
TK Zank U Zahringer C Beckmann G Pohnert W Boland H Holtorf R Reski J Lerchl E Heinz (2002) ArticleTitleCloning and functional characterisation of an enzyme involved in the elongation of Delta6-polyunsaturated fatty acids from the moss Physcomitrella patens Plant J 31 255–268 Occurrence Handle10.1046/j.1365-313X.2002.01354.x Occurrence Handle12164806
M Zeidler E Hartmann J Hughes (1999) ArticleTitleTransgene expression in the moss Ceratodon purpureus J Plant Physiol 154 641–650
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Thornton, L.E., Keren, N., Ohad, I. et al. Physcomitrella patens and Ceratodon purpureus, mosses as model organisms in photosynthesis studies. Photosynth Res 83, 87–96 (2005). https://doi.org/10.1007/s11120-004-5577-3
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DOI: https://doi.org/10.1007/s11120-004-5577-3