Summary
A technique has been developed for the isolation of large numbers of protoplasts from protonemal tissue of Physcomitrella patens, and for their regeneration to give whole plants. Somatic hybrids have been selected following treatment of mixtures of protoplasts from complementary auxotrophic strains with 50 mM CaCl2 at high pH. The hybrids have a morphology different from that of normal haploid strains, but similar to that of aposporously produced diploids. The progeny resulting from selffertilisation of the hybrids show a segregation which is consistent with their being the products of meioses in an autotetraploid.
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Anne, J., Peberdy, J.F.: Induced fusion of fungal protoplasts following treatment with polythylene glycol. J. gen. Microbiol. 92, 413–417 (1976)
Ashton, N.W.: Genetic studies on auxotrophic and developmental mutants in the moss, Physcomitrella patens. Ph.D. Thesis, University of Cambridge, 1974
Ashton, N.W., Cove, D.J.: The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens. Molec. gen. Genet. 154, 87–95 (1977)
Bopp, M., Jahn, H., Klein, B.: Eine einfache Methode, das Substrat während der Entwicklung von Moosprotonemen zu wechseln. Rev. Bryol. Lichen. 33, 219–223 (1964)
Carlson, P.S., Smith, H.H., Dearing, R.D.: Parasexual interspecific plant hybridisation. Proc. nat. Acad. Sci. (Wash.) 69, 2292–2294 (1972)
Engel, P.P.: The induction of biochemical and morphological mutants in the moss Physcomitrella patens. Amer. J. Bot. 55, 438–446 (1968)
Ferenczy, L., Kevei, F., Szegedin, M.: High frequency fusion of fungal protoplasts. Experientia (Basel) 31, 1028–1030 (1975)
Kao, K.N., Michayluk, M.R.: A method for high-frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)
Keller, W.A., Melchers, G.: The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28c, 737–741 (1973)
Melchers, G., Labib, G.: Somatic hybridisation of plant by fusion of protoplasts. 1. Selection of light resistant hybrids of “haploid” light sensitive varieties of tobacco. Molec. gen. Genet. 135, 277–294 (1974)
Schieder, O.: Selection of a somatic hybrid between auxotrophic mutants of Sphaerocarpos donnelli AUST. using the method of protoplast fusion. Z. Pflanzenphysiol. 74, 357–365 (1974)
Stumm, I., Meyer, Y., Abel, W.O.: Regeneration of the moss Physcomitrella patens (Hedw.) from isolated protoplasts. Plant Sci. Letters 5, 113–118 (1975)
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Grimsley, N.H., Ashton, N.W. & Cove, D.J. The production of somatic hybrids by protoplast fusion in the moss, Physcomitrella patens . Molec. Gen. Genet. 154, 97–100 (1977). https://doi.org/10.1007/BF00265582
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DOI: https://doi.org/10.1007/BF00265582