Abstract
Somatic embryogenesis and plant regeneration were induced from immature embryonal axes and immature cotyledons of peanut (Arachis hypogaea L. fastigata type cv JLM-1). Influence of different auxins, cytokinins and sugars on somatic embryogenesis from immature cotyledon explants was also investigated. Among the different auxins tested, 2,4-dichlorophenoxyacetic acid (2,4-d) was most effective, producing the highest frequency of responding cultures and highest average number of somatic embryos per responding culture, while dicamba, picloram, indolepropionic acid, α-naphthaleneacetic acid, 2,4,4-trichlorophenoxypropionic acid and α-naphthoxyacetic acid were also effective for embryogenesis. Indolebutyric acid, indoleacetic acid, p-chlorophenoxyacetic acid and trichlorophenoxyacetic acid were not beneficial. Among the four cytokinins tested, zeatin slightly enhanced the frequency of somatic embryogenesis, while kinetin, 6-γ-γ-dimethylallylaminopurine and benzyladenine were relatively inhibitory. Among the different carbon sources tested, sucrose was the best for embryo induction and at 6% sucrose the highest frequency of responding cultures and average number of somatic embryos per explant were obtained. For inducing embryogenesis from embryonal axes, 2,4-d was more effective than picloram. Highest plant conversion frequency from somatic embryos was obtained in presence of dicamba or NAA and using cotyledon explants.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxy-acetic acid
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- IPA:
-
3-indolepropionic acid
- 2iP:
-
6-γ-γ-dimethylallylaminopurine
- Dicamba:
-
3,6-dichloro-O-anisic acid
- pCPA:
-
p-chlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- NOA:
-
α-naphthoxyacetic acid
- 2,4,5-T:
-
2,4,5-trichlorophenoxyacetic acid
- 2,4,5-TP:
-
2,4,5-trichlorophenoxypropionic acid
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Eapen, S., George, L. Somatic embryogenesis in peanut: Influence of growth regulators and sugars. Plant Cell Tiss Organ Cult 35, 151–156 (1993). https://doi.org/10.1007/BF00032964
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DOI: https://doi.org/10.1007/BF00032964