Abstract
Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume. The cultures originated from three-week-old leaf callus. Several media including MS, B5, AA, SL, PCM, SH and L-6 were tested for their effects on cell growth. Maximum growth was observed in L-6 medium containing 44.5 μM 2,4-D. After 6 to 8 weeks the suspensions were filtered through 500, 250, 125 and 60 μm sieves, respectively, for four to five subcultures. An embryogenic cell line (VA-686) was obtained from the cell fraction collected below 250 μm. The VA-686 cell line is being maintained on L-6 medium with 4.5 μM 2,4-D and 2.3 μM Zeatin. Somatic embryogenesis was induced by transferring the cells to L-6 medium with 4.6 μM zeatin in which green cell clusters were produced. The somatic embryos developed from most of the cell clusters when plated on L-6 agar medium with 2.3 μM BA.
Plantlets were obtained from the embryos on L-6 medium with 10.0 μM IBA. The regenerated plants were grown to maturity in the greenhouse.
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Abbreviations
- BA:
-
Benzyladenine
- 2,4-D:
-
2,4- dichlorophenoxyacetic acid
- GA3 :
-
Gibberellic acid
- IBA:
-
Indole-3-butyric acid
- IPA:
-
Isopentenyladenine
- KN:
-
Kinetin
- NAA:
-
Napthaleneacetic acid
- AA:
-
Toriyama and Hinata, 1985
- SL:
-
Phillips and Collin, 1980
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Communicated by G. C. Phillips
A project sponsored by United States Agency for International Development, Washington D.C.
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Kumar, A.S., Gamborg, O.L. & Nabors, M.W. Plant regeneration from cell suspension cultures of Vigna aconitifolia . Plant Cell Reports 7, 138–141 (1988). https://doi.org/10.1007/BF00270124
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DOI: https://doi.org/10.1007/BF00270124