Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1532)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for theanalysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Similar content being viewed by others
Keywords
- EBV-associated diseases
- EBV-epithelial cell interactions
- purification-mass spectrometry methods
- 3D telomere FISH
- high-throughput RNA sequencing
- qPCR and nuclear run-on assay
- chromatin immunoprecipitation coupled sequencing (ChIP-Seq)
- Zta-DNA interactions
- tissue culture
- post-transplant lymphoproliferative diseases (PTLD)
- Good Manufacturing Practice-complaint
- infectious diseases
Table of contents (19 protocols)
Reviews
Editors and Affiliations
Bibliographic Information
Book Title: Epstein Barr Virus
Book Subtitle: Methods and Protocols
Editors: Janos Minarovits, Hans Helmut Niller
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6655-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6653-0Published: 22 November 2016
Softcover ISBN: 978-1-4939-8262-2Published: 04 May 2018
eBook ISBN: 978-1-4939-6655-4Published: 21 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 269
Number of Illustrations: 1 b/w illustrations, 21 illustrations in colour
Topics: Infectious Diseases