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Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments

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Epstein Barr Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1532))

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Abstract

DNA sequencing approaches originally developed in two directions, the chemical degradation method and the chain-termination method. The latter one became more widespread and a huge amount of sequencing data including whole genome sequences accumulated, based on the use of capillary sequencer systems and the application of a modified chain-termination method which proved to be relatively easy, fast, and reliable. In addition, relatively long, up to 1000 bp sequences could be obtained with a single read with high per-base accuracy. Although the recent appearance of next-generation DNA sequencing (NGS) technologies enabled high-throughput and low cost analysis of DNA, the modified chain-terminating methods are often applied in research until now. In the following, we shall present the application of capillary sequencing for the sequence characterization of viral genomes in case of partial and whole genome sequencing, and demonstrate it on the BARF1 promoter of Epstein Barr virus (EBV).

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Author information

Authors and Affiliations

  1. RT-Europe Nonprofit Research Ltd., Vár 2, Szigetköz Building, Mosonmagyaróvár, 9200, Hungary

    Kalman Szenthe Ph.D. & Ferenc Bánáti Ph.D.

Authors
  1. Kalman Szenthe Ph.D.
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  2. Ferenc Bánáti Ph.D.
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Editor information

Editors and Affiliations

  1. University of Szeged, Szeged, Hungary

    Janos Minarovits

  2. University of Regensburg, Regensburg, Germany

    Hans Helmut Niller

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Szenthe, K., Bánáti, F. (2017). Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments. In: Minarovits, J., Niller, H. (eds) Epstein Barr Virus. Methods in Molecular Biology, vol 1532. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6655-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6655-4_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6653-0

  • Online ISBN: 978-1-4939-6655-4

  • eBook Packages: Springer Protocols

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