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Bacterial Pathogenesis

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Pontus Nordenfelt

    1. Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Mattias Collin

    1. Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2674)

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About this book

This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Bacterial Pathogenesis: Methods and Protocols, Second Edition is a vital resource for researchers in the area of infection biology, as well as but not limited to, those working in the fields of microbiology, immunology, structural biology, molecular biology, genetics, imaging, and computational study.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Biofilms and Subcellular Compartments

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria

      • Supradipta De, Anders P. Hakansson
      Pages 3-32

    3. Formation and Analysis of Mono-species and Polymicrobial Oral Biofilms in Flow-Cell Models

      • Jessica Neilands, Gunnel Svensäter, Gabriella Boisen, Carolina Robertsson, Claes Wickström, Julia R. Davies
      Pages 33-54

    4. Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs)

      • Komal Umashankar Rao, Gabriela Godaly
      Pages 55-60

    5. Strategies to Isolate Extracellular Vesicles from Gram-Negative and Gram-Positive Bacteria

      • Ana Rita Narciso, Marie-Stéphanie Aschtgen
      Pages 61-70

  3. Bacterial Genetics, Genomics, and Phylogenetics

    1. Front Matter

      Pages 71-71
      Download chapter PDF

    2. Total Bacterial RNA Isolation and Northern Blotting Analysis

      • Jens Karlsson, Hannes Eichner, Edmund Loh
      Pages 73-85

    3. Phylogenetic Analysis of Bacterial Pathogen Genomes

      • Xavier Didelot
      Pages 87-99

    4. Determination of Growth Rate and Virulence Plasmid Copy Number During Yersinia pseudotuberculosis Infection Using Droplet Digital PCR

      • Tifaine Hechard, Helen Wang
      Pages 101-115

  4. Identification and Characterization of Bacterial Effector Proteins

    1. Front Matter

      Pages 117-117
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    2. Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence

      • Haniyeh Aghababa, Jacelyn M. S. Loh, Thomas Proft
      Pages 119-129

    3. Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae

      • Tova Lindh, Mattias Collin, Rolf Lood, Magnus Carlquist
      Pages 131-146

    4. Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-β-N-Acetylglucosaminidases

      • Jonathan J. Du, Diego Sastre, Beatriz Trastoy, Blaine Roberts, Daniel Deredge, Erik H. Klontz et al.
      Pages 147-167

    5. Identification of Substrates of Secreted Bacterial Protease by APEX2-Based Proximity Labeling

      • Yanxuan Xie, Yang Mao, Zong-Wan Mao, Wei Xia
      Pages 169-179

    6. Affinity-Purification Combined with Crosslinking Mass Spectrometry for Identification and Structural Modeling of Host–Pathogen Protein–Protein Complexes

      • Lotta J. Happonen
      Pages 181-200

    7. Elucidating the Stoichiometries of Host–Pathogen Protein Interactions with Mass Photometry

      • Emma-Jayne Proctor, Sandeep Satapathy, Martina Sanderson-Smith
      Pages 201-208

  5. Analysis of Host Responses to Bacteria

    1. Front Matter

      Pages 209-209
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    2. Analysis of Neutrophil and Monocyte Inflammation Markers in Response to Gram-Positive Anaerobic Cocci

      • Tobias Schmidt, Ariane Neumann
      Pages 211-220

    3. Quantification of Phagocytosis Using Flow Cytometry

      • Therese de Neergaard, Pontus Nordenfelt
      Pages 221-234
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Editors and Affiliations

  • Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden

    Pontus Nordenfelt, Mattias Collin

Bibliographic Information

  • Book Title: Bacterial Pathogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Pontus Nordenfelt, Mattias Collin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3243-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3242-0Published: 01 June 2023

  • Softcover ISBN: 978-1-0716-3245-1Published: 02 June 2024

  • eBook ISBN: 978-1-0716-3243-7Published: 31 May 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 337

  • Number of Illustrations: 4 b/w illustrations, 66 illustrations in colour

  • Topics: Microbiology, Biomedicine, general

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