Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2582)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.
Similar content being viewed by others
Keywords
Table of contents (28 protocols)
-
Pathophysiology
Editors and Affiliations
Bibliographic Information
Book Title: CCN Proteins
Book Subtitle: Methods and Protocols
Editors: Masaharu Takigawa
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2744-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-2743-3Published: 13 November 2022
Softcover ISBN: 978-1-0716-2746-4Published: 14 November 2023
eBook ISBN: 978-1-0716-2744-0Published: 12 November 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 430
Number of Illustrations: 20 b/w illustrations, 66 illustrations in colour