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Glycovirology

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Yasuo Suzuki

    1. Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2556)

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About this book

This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology.

 

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Comprehensive Cellular Glycan Profiling of Glycoproteins and Glycosphingolipids by Glycoblotting and BEP Methods

    • Hisatoshi Hanamatsu, Jun-ichi Furukawa
    Pages 1-18

  3. High-Performance Liquid Chromatography Mapping Method for the Structural Analysis of N-Glycans at the Molecular, Cellular, and Tissue Levels

    • Taiki Saito, Hirokazu Yagi
    Pages 19-30

  4. Detection of Sialic Acids on the Cell Surface Using Flow Cytometry

    • Hirohito Ishigaki, Yasushi Itoh
    Pages 31-35

  5. Lectin Staining to Detect Human and Avian Influenza Virus Receptors in the Airway of Nonhuman Primates

    • Misako Nakayama, Yasushi Itoh
    Pages 37-43

  6. Preparation of Glycan Arrays Using Glycopeptides Derived From Biomaterials

    • Shin-ichi Nakakita, Yukari Nakakita, Ryohsuke Kurihara, Jun Hirabayashi
    Pages 45-58

  7. Glycan Profiling of Viral Glycoproteins with the Lectin Microarray

    • Takahiro Hiono, Atsushi Kuno
    Pages 59-68

  8. Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application for Glycobiology Research

    • Takashi Kuzuhara
    Pages 69-78

  9. Receptor-Binding Specificity of Influenza Viruses

    • Masaki Imai, Kosuke Takada, Yoshihiro Kawaoka
    Pages 79-96

  10. Functional Analysis of Sulfatide in Influenza A Virus Infection and Replication

    • Tadanobu Takahashi, Yuuki Kurebayashi, Takashi Suzuki
    Pages 97-122

  11. Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry

    • Erik de Vries, Hongbo Guo, Wenjuan Du, Mengying Liu, Frank J. M. van Kuppeveld, Cornelis A. M. de Haan
    Pages 123-140

  12. Receptor-Binding Assay for Avian Influenza Viruses

    • Takahiro Hiono, Daiki Kobayashi
    Pages 141-148

  13. Characterization of Influenza Virus Binding to Receptors on Isolated Cell Membranes

    • Mikhail N. Matrosovich, Alexandra S. Gambaryan
    Pages 149-168

  14. Characterization of Human Parainfluenza Virus Receptor Using Terminal Sialic Acid Linkage-Modified Cells

    • Keijo Fukushima, Tadanobu Takahashi, Takashi Suzuki
    Pages 169-178

  15. Solid-Phase Binding Assay for Ganglioside Binding of Human Respiroviruses

    • Takashi Suzuki
    Pages 179-186

  16. Evaluation of the Glycan-Binding and Esterase Activities of Hemagglutinin-Esterase-Fusion Glycoprotein from Influenza D Virus

    • Hao Song, George F. Gao
    Pages 187-203

  17. Roles of Glycans and Non-glycans on the Epithelium and in the Immune System in H1–H18 Influenza A Virus Infections

    • Nongluk Sriwilaijaroen, Yasuo Suzuki
    Pages 205-242

  18. Roles of Sialyl Glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 Infections

    • Nongluk Sriwilaijaroen, Yasuo Suzuki
    Pages 243-271

  19. Enzymatic Substrates and Fluorescence Imaging of Influenza Virus Sialidase

    • Yuuki Kurebayashi, Tadanobu Takahashi, Takashi Suzuki
    Pages 273-286

  20. Assays for Determining the Sialidase Activity of Influenza Viruses and Monitoring Influenza Virus Susceptibility to Neuraminidase Inhibitors

    • Emi Takashita
    Pages 287-302
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Editors and Affiliations

  • Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan

    Yasuo Suzuki

Bibliographic Information

  • Book Title: Glycovirology

  • Book Subtitle: Methods and Protocols

  • Editors: Yasuo Suzuki

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2635-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2634-4Published: 30 September 2022

  • Softcover ISBN: 978-1-0716-2637-5Published: 02 October 2023

  • eBook ISBN: 978-1-0716-2635-1Published: 29 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 357

  • Number of Illustrations: 42 b/w illustrations, 68 illustrations in colour

  • Topics: Virology, Medical Biochemistry

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