Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2544)
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About this book
This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.
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Keywords
Table of contents (21 protocols)
Editors and Affiliations
Bibliographic Information
Book Title: Hepatocytes
Book Subtitle: Methods and Protocols
Editors: Naoki Tanimizu
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2557-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2556-9Published: 21 September 2022
Softcover ISBN: 978-1-0716-2559-0Published: 22 September 2023
eBook ISBN: 978-1-0716-2557-6Published: 20 September 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 281
Number of Illustrations: 7 b/w illustrations, 42 illustrations in colour
Topics: Physiology, Biomedicine, general