Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2517)
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About this book
This volume covers the latest experimental protocols used by researchers to study the recently emerged fungal pathogen of humans, Candida auris, and also explores the pathogen’s antifungal resistance, virulence, and pathogenicity. The chapters in this book cover topics such as the identification of C. auris in clinic samples by PCR and mass spectrometry; molecular typing to identify clade affiliation; genetic manipulation; genome analysis by bioinformatics; and phenotypic characterization using various biochemical, mass spectrometry, microscopy, and flow cytometry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Candida auris: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field.
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Keywords
Table of contents (26 protocols)
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Isolation and Identification of Candida auris
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Testing Susceptibility of Candida auris to Antifungal Drugs
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Genetic Manipulation of Candida auris
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Phenotypic and Biochemical Characterization of Candida auris
Editors and Affiliations
Bibliographic Information
Book Title: Candida auris
Book Subtitle: Methods and Protocols
Editors: Alexander Lorenz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2417-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2416-6Published: 09 June 2022
Softcover ISBN: 978-1-0716-2419-7Published: 10 June 2023
eBook ISBN: 978-1-0716-2417-3Published: 08 June 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 342
Number of Illustrations: 16 b/w illustrations, 60 illustrations in colour
Topics: Microbiology, Mycology