Abstract
The recently emerged human pathogenic yeast Candida auris has become a major global threat. As compared to other Candida species, C. auris often displays a high level of resistance to commonly used antifungals and poses additional therapeutic challenges. There is a great need to understand the molecular basis of its success as a drug-resistant human pathogen. The study of condition-specific gene expression can provide good cues of regulatory circuitry governing high drug resistance. Here, we describe the protocol of quantitative reverse transcription PCR (RT–qPCR) which can be conveniently employed as a highly reproducible method for measuring individual transcripts in C. auris cells.
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Wasi, M., Nair, R., Khandelwal, N.K., Prasad, R. (2022). Measuring Gene Expression via mRNA Abundance in Candida auris. In: Lorenz, A. (eds) Candida auris. Methods in Molecular Biology, vol 2517. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2417-3_11
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DOI: https://doi.org/10.1007/978-1-0716-2417-3_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2416-6
Online ISBN: 978-1-0716-2417-3
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