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Affinity Chromatography

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. B. Vijayalakshmi Ayyar

    1. Molecular Virology and Microbiology, Baylor College of Medicine, Houston, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Sushrut Arora

    1. Astero Erado Inc, Houston, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2466)

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About this book

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Applications of Affinity Chromatography

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Antibody Purification Using Affinity Chromatography

      • Arabelle Cassedy, Richard O’Kennedy
      Pages 3-22

    3. TIM4-Affinity Methods Targeting Phosphatidylserine for Isolation or Detection of Extracellular Vesicles

      • Takeshi Yoshida, Rikinari Hanayama
      Pages 23-36

    4. Application of Affitins for Affinity Purification of Proteins

      • Barbara Mouratou, Frédéric Pecorari
      Pages 37-48

    5. Identification of Clusterin as a Major ABri- and ADan-Binding Protein Using Affinity Chromatography

      • Agueda Rostagno, Miguel Calero, Jorge Ghiso
      Pages 49-60

    6. Application of a Novel CL7/Im7 Affinity System in Purification of Complex and Pharmaceutical Proteins

      • Louise T. Chow, Dmitry G. Vassylyev
      Pages 61-82

    7. Positive and Negative Affinity Chromatography to Purify Norovirus P-Domain Protein

      • Wilhelm Salmen
      Pages 83-92

    8. Robotic Affinity Purification of Soluble and Insoluble Recombinant Glutathione-S-Transferase Fusion Proteins

      • Robert O. J. Weinzierl
      Pages 93-109

    9. Application of Immunoaffinity Mass Spectrometry (IA-MS) for Protein Biomarker Quantification

      • Joe Palandra, Nikolaos Psychogios, Hendrik Neubert
      Pages 111-119

    10. Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP)

      • Markus Niehaus, Marco Herde
      Pages 121-133

    11. Arginine-Affinity Chromatography for Nucleic Acid (DNA and RNA) Isolation

      • Rita Carapito, Joana F. A. Valente, João A. Queiroz, Fani Sousa
      Pages 135-144

    12. Purification and Analysis of Nucleotides and Nucleosides from Plants

      • Henryk Straube, Marco Herde
      Pages 145-155

  3. Ligand Selection and Affinity Matrix Preparation for Affinity Chromatography

    1. Front Matter

      Pages 157-157
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    2. Ligand Selection for Affinity Chromatography Using Phage Display

      • Krištof Bozovičar, Peter Molek, Barbara Jenko Bizjan, Tomaž Bratkovič
      Pages 159-185

    3. Selection and Application of Aptamer Affinity for Protein Purification

      • Ana Paula de Jesus Santos, Ágatha Oliveira-Giacomelli, Vanessa Karen de Sá, Isis Cristina do Nascimento, Erika de Simone Molina, Henning Ulrich
      Pages 187-203

    4. Entrapment of Proteins Within Columns for High-Performance Affinity Chromatography

      • Saumen Poddar, Sadia Sharmeen, David S. Hage
      Pages 205-227

    5. Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation

      • Kishore K. R. Tetala
      Pages 229-240

    6. Ligand Immobilization Methods for Affinity Chromatography

      • Chandra K. Dixit
      Pages 241-247

    7. Preparation of Molecularly Imprinted Poly(N-Isopropylacrylamide) Thermosensitive Based Cryogels

      • Işık Perçin, Neslihan Idil, Adil Denizli
      Pages 249-260
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Editors and Affiliations

  • Molecular Virology and Microbiology, Baylor College of Medicine, Houston, USA

    B. Vijayalakshmi Ayyar

  • Astero Erado Inc, Houston, USA

    Sushrut Arora

Bibliographic Information

  • Book Title: Affinity Chromatography

  • Book Subtitle: Methods and Protocols

  • Editors: B. Vijayalakshmi Ayyar, Sushrut Arora

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2176-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2175-2Published: 19 May 2022

  • Softcover ISBN: 978-1-0716-2178-3Published: 20 May 2023

  • eBook ISBN: 978-1-0716-2176-9Published: 18 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour

  • Topics: Analytical Chemistry, Biological Techniques

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