Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2466)
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About this book
This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.Similar content being viewed by others
Keywords
Table of contents (18 protocols)
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Ligand Selection and Affinity Matrix Preparation for Affinity Chromatography
Editors and Affiliations
Bibliographic Information
Book Title: Affinity Chromatography
Book Subtitle: Methods and Protocols
Editors: B. Vijayalakshmi Ayyar, Sushrut Arora
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2176-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2175-2Published: 19 May 2022
Softcover ISBN: 978-1-0716-2178-3Published: 20 May 2023
eBook ISBN: 978-1-0716-2176-9Published: 18 May 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 278
Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour