Skip to main content
SpringerLink
Log in

Fluorescent Microscopy

  • Book
  • © 2022

Overview

Editors:
  1. Bryan Heit

    1. Department of Microbiology and Immunology, Center for Human Immunology, The University of Western Ontario, London, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2440)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. 

Similar content being viewed by others

Keywords

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Basic Fluorescence Imaging

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Fluorescence Microscopy: A Field Guide for Biologists

      • Lucy H. Swift, Pina Colarusso
      Pages 3-39

    3. Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy

      • Richard Manivanh, Seema S. Lakdawala, Jennifer E. Jones
      Pages 41-56

    4. Optimizing Long-Term Live Cell Imaging

      • Alex Lac, Austin Le Lam, Bryan Heit
      Pages 57-73

  3. Quantitative Approaches

    1. Front Matter

      Pages 75-75
      Download chapter PDF

    2. Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy

      • Janhavi Nagwekar, Caterina Di Ciano-Oliveira, Gregory D. Fairn
      Pages 77-90

    3. Bimolecular Fluorescence Complementation to Visualize Protein–Protein Interactions in Cells

      • Cassandra R. Edgar, Jimmy D. Dikeakos
      Pages 91-97

    4. Monitoring Cellular Responses to Infection with Fluorescent Biosensors

      • Amena Aktar, Kasia M. Wodz, Bryan Heit
      Pages 99-114

    5. Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF)

      • Erika Jang, Siavash Ghaffari, Warren L. Lee
      Pages 115-124

    6. Measurement of Minute Cellular Forces by Traction Force Microscopy

      • Valentin Jaumouillé
      Pages 125-139

  4. Tissue and Live Animal Imaging

    1. Front Matter

      Pages 141-141
      Download chapter PDF

    2. Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues

      • Lane B. Buchanan, Zhongtian Shao, Yuan Chung Jiang, Abbie Lai, Thomas J. Hope, Ann M. Carias et al.
      Pages 143-164

    3. Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin

      • Madison Turk, Jeff Biernaskie, Douglas J. Mahoney, Craig N. Jenne
      Pages 165-180

    4. Quantifiable Intravital Light Sheet Microscopy

      • Holly C. Gibbs, Sreeja Sarasamma, Oscar R. Benavides, David G. Green, Nathan A. Hart, Alvin T. Yeh et al.
      Pages 181-196

    5. Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing

      • Frank L. Jalufka, Sun Won Min, Madison E. Platt, Anna L. Pritchard, Theodore E. Margo, Alexandra O. Vernino et al.
      Pages 197-209

    6. Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

      • Ory Perelsman, Shoh Asano, Limor Freifeld
      Pages 211-222

  5. Super-Resolution Approaches

    1. Front Matter

      Pages 223-223
      Download chapter PDF

    2. Super-Resolution Radial Fluctuations (SRRF) Microscopy

      • Jayme Salsman, Graham Dellaire
      Pages 225-251

    3. Sample Preparation for Multicolor STED Microscopy

      • Walaa Alshafie, Thomas Stroh
      Pages 253-270
Back to top

Editors and Affiliations

  • Department of Microbiology and Immunology, Center for Human Immunology, The University of Western Ontario, London, Canada

    Bryan Heit

Bibliographic Information

  • Book Title: Fluorescent Microscopy

  • Editors: Bryan Heit

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2051-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2050-2Published: 27 February 2022

  • Softcover ISBN: 978-1-0716-2053-3Published: 28 February 2023

  • eBook ISBN: 978-1-0716-2051-9Published: 26 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 370

  • Number of Illustrations: 27 b/w illustrations, 70 illustrations in colour

  • Topics: Analytical Chemistry, Materials Science, general

Publish with us

Policies and ethics

Back to top

Search

Navigation