Abstract
Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
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Acknowledgments
The authors thank Ms. Yarden Levinsky for help with demonstration of the expansion procedure, Mr. Nitay Aspis for ExM imaging of a larval zebrafish brain and Dr. Nitsan Dahan from the LS&E microscopy core facility for technical assistance with light-sheet microscopy imaging. We also thank the Baier lab for kindly providing us with the s1181Et;UAS:Kaede fish [26] shown in Fig. 3a and the Engert lab for kindly providing us with the actb2:H2B-EGFP fish [27] shown in Fig. 3b. Limor Freifeld is funded by the Zuckerman STEM Leadership Program.
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Perelsman, O., Asano, S., Freifeld, L. (2022). Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos. In: Heit, B. (eds) Fluorescent Microscopy. Methods in Molecular Biology, vol 2440. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2051-9_13
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DOI: https://doi.org/10.1007/978-1-0716-2051-9_13
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