Abstract
Fruits and vegetables (F&V) are living tissues that continue to respire after picking, and while this can be controlled by freezing, for the conservation of its components, maintaining its sensory quality. This review aims (i) to review the use of novel combined technologies used in the F&V freezing process, (ii) to evaluate its unconventional variants to obtain high-quality frozen products, including different aspects that influence this thermal process. The basic principles and uses of new technologies (i.e., ultrasound, magnetic fields, high pressure, microwaves, osmotic dehydration, isochoric freezing and cryogenic freezing and unconventional processes) are described. Moreover, was evaluated the impact of each technology on the control of the formation and growth of ice crystals, and its impact on the microstructure and quality characteristics of F&V, as well as their proposed mathematical models. It is concluded that new technologies combined with freezing have a positive and promising effect on process optimization, since their application can minimize the negative effects of traditional freezing methods.
Graphical Abstract
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Fruits and vegetables (F&V) are living organisms, metabolically active and perishable foods with a soft and delicate texture that contain high amounts of water in their composition (from 70 to 90%), characteristics that make them susceptible to mechanical damage, microbial attack and, consequently, loss of its organoleptic [1] and bioactive quality [2]. Likewise, its intense rate of respiration (generation of heat and carbon dioxide (CO2) by oxidation of its stored nutrients) and transpiration (elimination of water vapor for the regulation of internal thermal balance) lead to deterioration, senescence, and reduction of its useful life [3, 4].
Freezing process is a conventional preservation method widely used to extend the shelf life of highly perishable foods after harvest. It consists of subjecting the food to temperatures below 0 °C, causing a reduction in the mobility and water activity (aw) and, producing a decrease in chemical and enzymatic reactions and proliferation of microbial growth [5, 6]. In this context, freezing represents a common and viable method for prolonging the shelf life and also is non-invasive process, that preserves the intrinsic properties of the food with it the nutritional and organoleptic qualities, maintaining food safety and security [7]. However, the nucleation and formation of ice crystals inside the tissues is associated with various factors of the food (size, shape, structure, and composition) and the freezing method [8, 9]. During freezing process, heat and mass transfer phenomena are strongly linked to properties such as surface area, composition, density, and porosity of the food. These properties have a significant impact on the cost and efficiency of the finished product process and its nutritional and organoleptic characteristics. For this reason, the numerical models established for the freezing process are based on said transport phenomena, which will allow predicting, calculating freezing times and temperatures that are essential to maintain effective control of the process [10, 11].
There are efforts to optimize the freezing process in terms of speed that lead to reducing the structural damage produced in conventional freezing [12]. However, optimizing the process is complicated due to the ionic composition of the F&V, which causes high osmotic stress, consequently, an increase in the diffusion coefficient of cellular water, resulting in smaller ice crystals, which would solve the problem of cellular microstructure degradation [13]. Among the novel technologies, ultrasound-assisted freezing has shown excellent results due to the acoustic cavitation effect, which promotes ice nucleation by microbubbles and generating very small ice crystals [14]. Magnetic field assisted freezing has gained quite a lot of popularity, which through vibrating movements in the water molecule causes the water to remain liquid in a subcooled state and, furthermore, suppresses the nucleation of large ice crystals, favoring the formation of fine and microscopic crystals [15]. High pressure freezing is also an interesting technology with a great potential, its effects on the chemical and microbiological aspects of food are governed by Le Chatelier’s principle [16]. The high pressure allows accelerating the nucleation stage, giving rise to the formation of a homogeneous matrix composed of numbers of ice crystals of microscopic size that will preserve the textural quality. Electromagnetic wave-assisted freezing is considered a technology with great potential, which causes friction between water molecules to produce a heating effect, thus, the energy is dissipated by the microwave, inducing the partial melting of the ice crystals [17, 18], since it allows control of the stage of formation of ice crystals during the process [19]. Osmotic dehydration as a pretreatment to the freezing process is based on the elimination of a large part of the water present in food by means of osmotic solutions with a high concentration of solutes for a period of time and a determined temperature, favoring the least amount of ice crystals, thus avoiding microstructural damage and preserving the organoleptic attributes, mainly in the color of the product [20].
There are other types of unconventional freezing such as cryogenic freezing, which consists of a very fast reduction of the temperature below the freezing threshold of the food, achieving beneficial effects such as reducing thawing losses, improving the appearance of F&V and maintaining the complete cell structure [21, 22]. Likewise, isochoric freezing is capable of completely preserving the cellular microstructure of the food, reduces microbial attack and allows considerable energy savings [23,24,25]. For all the above-mentioned, the aims of this review are to consolidate the knowledge related to fundamentals of the freezing process, the numerical models that explain this unit operation, the transport phenomena that occur, and the novel technologies combined with the freezing process.
Fundamentals of Freezing in Fruits and Vegetables (F&V)
Although freezing consists of subjecting the food to temperatures below 0 °C, internationally the reference temperature for freezing is -18 °C; at this temperature a large percentage of water contained in the food has been transformed into ice crystals [26]. However, since water contains dissolved solids, and as it freezes, the solutions that remain in a liquid state become more concentrated in solutes. This increase in concentration produces a phenomenon called cryoscopic descent, that is, as solutions are concentrated in dissolved solids, the temperature at which freezing occurs decreases, this explains why freezing occurs at temperatures below 0 °C [11, 27]. After the freezing process it is possible to notice three significant changes in the plant tissue, (1) dehydration of the cell due to the location of the ice nucleation, (2) solute damage induced by the increase in intracellular fluid concentration during freezing and (3) mechanical damage through stress imparted by the expansion of the ice phase [28] closely related to the location of nucleation, within cells (intracellular nucleation) or outside of cells (extracellular nucleation). Extracellular nucleation is more convenient for the optimal conservation of plant tissue because it has heterogeneous nucleation sites [29].
A homogeneous nucleation in the intercellular space with formation of smaller ice crystals than the size of the cell, is favorable. This would avoid perforation of the vacuole membrane, maintaining its structure and cellular integrity. On the contrary, if this does not happen, the formation of large ice crystals would cause the release of simple sugars, minerals, vitamins, polysaccharides, bioactive compounds, pigments, among others, from the vacuole into the extracellular space. This would negatively affect the osmotic balance, the turgidity of the cell and the functionality of the plant tissue, thus leading to the detriment of the organoleptic properties of texture, aroma and flavor of the F&V. In addition, the stability of bioactives would be altered, compromising the effectiveness of their antioxidant capacity and other chemoprotective properties favorable to human health [30,31,32,33,34]. Thus, the faster the extracellular ice formation occurs, the less damage to the cell walls of the food, whereas the opposite effect would occur if the process is done slowly [5, 35].
On the other hand, during the freezing process synchronous mass and heat transfer phenomena occur in non-steady state. The food loses heat by convection through its surface and by conduction from its interior [36]. Three stages can be distinguished in this process: a) pre-cooling, b) phase change and c) post-cooling [37, 38] indicate that Plank, in 1913, proposed a numerical model to predict the freezing time, being one of the most used mathematical models due to its ease. Based on this model, new numerical models have been developed. For example, Ede in 1949 adapted this equation for use in food (Eq. 1):
where: \({t}_{f}\) is the freezing time, \({\rho }_{f}\) is the density of the frozen material, \({L}_{f}\) is the latent heat of the food (kJ/kg), \({T}_{f}\) is the freezing temperature (°C), \({T}_{a}\) is the freezing air temperature (\(^\circ{\rm C}\)), \(h\) is the convection heat transfer coefficient for the material (\({w/m}^{2}\) °C), \(a\) is the thickness or diameter of the object (m), \(k\) is the thermal conductivity of the frozen material (\(w/m^\circ C\)), and the constants P’ y R’ whose values are according to the form of the product: P’ = ½, R’ = 1/8 for the infinite plate; P’ = ¼, R’ = 1/16 for infinite cylinder; and P ‘ = 1/6 y R’ = 1/24 for the sphere or cube.
The F&V represent a group of highly perishable foods due to their high-water content, up to 90%, which makes them susceptible to attack by microorganisms such as bacteria, fungi and enzymes [3, 4]. The freezing of F&V has become one of the most favorable technologies in the food industry, due to this process it is possible to store F&V seasonal, in order to extend its consumption time [39]. However, the freezing of F&V is very complex and different from the freezing of a drop of water due to the solutes present. In F&V the only thing that changes phase is pure water, the extracellular solution is concentrated in solids and the system becomes unbalanced, then, to try to restore the equilibrium of concentrations, water begins to migrate from inside the cell, that is, from the vacuole through the tonoplast into the cytoplasm and then into the intercellular space through the cell membrane and cell wall, and into the extracellular space through mechanisms called osmotic [11].
Novel Freezing Technologies
Ultrasound-Assisted Freezing
Otero et al. [40] described ultrasound as acoustic waves which are of low frequency (\(20 - 100000 Hz\)) and high intensity (generally \(<1 W/{cm}^{2}\)). Ultrasound is generated by the attraction of polarized molecules in the high-frequency electric field, which causes elastic deformation of ferroelectric materials. Zhang et al. [41] mention that these waves induce a series of compression and rarefaction cycles during their propagation. On the other hand, the ultrasonic effect is linked to the cavitation phenomenon that occurs when a high ultrasonic power is reached during ultrasound transmission [42]. The attractive forces cause the formation of bubbles or cavities in two ways: (a) stable, when this phenomenon occurs gradually in a way that the bubbles do not reach the critical size to implode, or (b) transient, when the bubbles reach a critical size and implode violently [41]. The application of power ultrasound in the food freezing process, both solid and liquid, has shown to have relevant effects in optimizing this process. During freezing, ultrasonic irradiation triggers cavitation and micro-flow effects, which increase the rate of heat and mass transfer. It also increases the nucleation rate, which promotes the formation of ice crystals to occur microscopically and uniformly distributed in the microstructure of the food, thus considerably reducing structural damage (Fig. 1).
However, for its application to result in beneficial and non-adverse effects, the different ultrasonic parameters that influence the efficiency of the freezing process must be considered. One of them, the ultrasonic intensity, if this also increases the efficiency of the process, but also increases the thermal effect, which impairs said efficiency. For this reason, it is essential to maintain a balance between the ultrasonic intensity and the thermal effect in such a way that the efficiency of the freezing process is preserved [41, 43, 44]. Likewise, in the freezing of F&V it is important to take into account the percentage of void that the plant tissues present; the greater the vacuum, the less effective the ultrasound treatment [45]. Figure 2A represents the way in which ultrasound influences ice crystals, and how the cavitation phenomenon develops. The effects of ultrasonic irradiation on the freezing and quality of strawberries, blueberries, apple, pear, Hami melon, broccoli, and red radish frozen by ultrasound-assisted immersion have been evaluated (Table 1). From these studies, it has been verified that the increase in the ultrasonic intensity decreases the time necessary to reach the supercooling stage, thus reducing the freezing time. Likewise, it improves the nucleation rate and the growth rate of the crystals, therefore, the application of this technology allows to improve the quality of frozen F&V and reduces the loss due to dripping and nutrients [46,47,48].
On the other hand, Zhang et al. [41] mention that the ultrasonic parameters depend on the nature of the food that will be subjected to said procedure, because their intrinsic characteristics and also influence the efficiency of the process. Cong et al. [79] established a mathematical model for the quantitative analysis of the heat and mass transfer of the drops in the ultrasonic-assisted freezing process, a model that allows knowing the influence of the ultrasonic effect on the performance of the heat and mass transfer (Eq. 2):
where: \({R}_{b}\) is the bubble radius, \({R}_{0}\) is the initial bubble radius, \({P}_{0}\) is the constant hydrostatic pressure, \({P}_{d}\) is the liquid vapor pressure, \(\mu\) is the dynamic viscosity of water, \({P}_{a}\) is the ultrasonic pressure, \(d\) represents the droplet, \(\rho\) is the density.
Alternatives are currently being studied to improve the effect of cavitation, since it plays an important role in optimizing the freezing process. It has been shown that cavitation affects the efficiency of the freezing rate and the acceleration of the formation of ice crystals [80]. A research carried out by Jiang et al. [81], evaluated the effect of pre-injection of pressurized CO2 combined with ultrasound-assisted immersion freezing on the microstructure of sweet melon, obtaining significantly positive effects. It was determined that the application of pressurized CO2 contributes to the improvement of the ultrasonic cavitation effect, consequently, it improves the formation and growth of ice crystals, allowing to reduce the microstructural damage of the product more effectively. However, despite being a remarkable technology for the optimization of the freezing process, it has only been used on a laboratory scale because considerable funding is required for the development of equipment that operates on an industrial scale [49].
Magnetic Field-Assisted Freezing
Magnetic fields are created by passing an electric current through a coil, which acts as an electromagnetic field. To create the magnetic field in an electromagnet, a coil of wire is wound around the magnetic core and when an electric current is passed through the wire, the magnetic field from all the turns of the wire passes through and penetrates the iron coil. This causes the domains to rotate and small magnetic fields to form from the core, creating the geomagnetic field effect [82]. Magnetic fields cause changes in the physical and chemical properties of liquid water molecules that are frozen through mechanisms of vibration, orientation, and rotation of their dipoles. This results in the magnetisation of water and alters its properties, including specific heat capacity, surface tension, conductivity, viscosity, and diffusion coefficient [52]. They also influence the distribution of electron clouds within water molecules (charge), the network of hydrogen bonds between molecules and the interactions/clusters between molecules and ions in aqueous solutions, which influences the kinetics of freezing.
During the freezing process, this technology strengthens the hydrogen bonds between water molecules, which enhances stability and order. This leads to a high level of supercooling, creating microscopic, fine, and evenly dispersed ice crystals in the food [83]. This protects the cell membranes of the F&V tissue, thereby enhancing the texture, taste and appearance of frozen foods [15, 84]. However, it is important to acknowledge that the impact of the magnetic field is contingent on its intensity, as well as the length and temperature of exposure during the procedure [82]. Figure 2B shows the action mechanisms of magnetic fields on water molecule clusters.
A study was conducted to compare the impact of permanent magnetic field (PMF) and alternating magnetic field (AMF) assisted freezing on the microstructure of blueberries showed that applying AMF at 0.05 mT led to a 55.8% reduction in ice crystals. However, as AMF intensity increased, the phase change time also escalated, promoting the growth of ice crystals in food freezing, hence conveying an undesirable outcome. While in PMF significantly reduced the size of crystals, as the intensity was increased. An optimal PMF intensity for blueberries in terms of freezing parameters and microstructure was found to be 10 mT, resulting in a 33.6% reduction in crystal size [55]. Similar research was conducted on cherries, indicating that PMF-assisted freezing requires less energy than AMF-assisted freezing. In cherry, as well as blueberry, the optimal PMF intensity was 10mT, resulting in a reduction of approximately 67% in ice crystals. The use of both types of magnetic fields led to a decrease in nutrient loss, with PMF resulting in lower losses than AMF [56].
Similarly, the effects of applying AMF to freeze minimally processed guava were evaluated, and positive results were obtained when 7.02 mT of AMF was used. Both phase change time and drip losses were reduced, and texture properties were better preserved. However, the precise effects could not be described as there is still insufficient study material on real food matrices with a wide range of magnetic parameters [15]. The effects of static magnetic field (SMF) application have been investigated in several F&V including apple, peach, cucumber, Indian jujube, broccoli, and cauliflower. The results showed that the optimal SMF intensity varied depending on the F&V studied. Furthermore, SMF application led to a decrease in respiratory intensity, membrane permeability y ice crystal size, which had a positive effect the preservation of the quality and microstructure of the Fruits and delicate vegetables such as spinach [31, 58, 85, 86]. However, in cherry tomatoes, SMF and AMF were found to increase the enzymatic activity of catalase, which is an enzyme that delays ripening. Therefore, these techniques can be used to control ripening during postharvest of fruit [57].
High-Pressure-Assisted Freezing
The use of pressure in the freezing process has great potential, as the pressure exerted (150 to 760 MPa) plays a key role in the transition from water to ice (Fig. 2C). Its application can improve the characteristics of the ice crystals formed during the process, as well as the freezing and thawing kinetics [83]. Acording to Sanz and Otero [87], three different high-pressure freezing processes can be distinguished (Fig. 2C), depending on how the phase transition occurs: 1. High-pressure-assisted freezing (HPAF), 2. High-pressure-induced freezing (HPIF), and 3. High-pressure-shift freezing (HPSF). In HPAF, the phase transition occurs at a constant pressure above atmospheric pressure, as the temperature drops below the corresponding freezing point, ice I known as ice polymorphs are obtained. Under HPAF conditions (200 MPa), the point of freezing of water will be very low (-22 °C), which reduces the water crystallisation but increases its vitrification upon freezing. Consequently, the formation and configuration of crystals is different, increasing the density of water molecules and preventing the expansion of their volume. Ice crystals formed under HAPF can be produced by two pathways, which lead to the formation of ice I (Fig. 2C—green line A to E) and ice V (Fig. 2C—dashed green line—a to e). Such thermodynamic water conditions can prevent mechanical damage caused by ice crystals in the F&V tissue.
Samples subjected to HPIF undergo a phase transition during the freezing process, which begins with a gradual increase in pressure (300 MPa), then the sample temperature decreases at a constant pressure, at which point the pressure gradually increases (up to 600 MPa), initiating the transition phase, leading to nucleation and formation of ice V (at -20 °C and 600 MPa) (Fig. 2C – red line A’’ to D’’) [51]. For the desired ice polymorph, compression can be performed directly at the target pressure (770 MPa) where ice VI is obtained (Fig. 2C red dashed line - a’’ to b’’). In the case of an HPSF, there is a slight increase in sample temperature due to pressurisation (210 MPa). Then, when the system pressure is released (fully or partially), the phase transition begins, increasing the freezing point and, suddenly and instantaneously, supercooling and expansion occurs, where the water at this point is still liquid (Fig. 2C, blue line C’-D’). Subsequently, a rapid nucleation starts after a slight heating (blue line D’-E’) and an immediate subsequent cooling (blue line E’-F), forming fine and uniform crystals of ice I. These pressure changes reduce the phase transition time and prevent sample heating, which is especially suitable for samples of large size or low thermal conductivity [87, 88].
It is important to note that the effects of high pressure on food chemistry and microbiology are based on the Le Chatelier’s principle. When the equilibrium of a system is disturbed, phenomena such as phase transitions, chemical reactivity, changes in molecular configuration and chemical reactions occur, which are accompanied by a decrease in volume, but which are counteracted by reactions involving an increase in volume. In that regard, all cellular components are affected by the resulting high pressure, including the cell membrane and its proteins (denaturation), enzymes (inhibition) and ribosomes, as well as the entire cell metabolism [16, 89]. For the mathematical modelling of high-pressure freezing processes, Sanz and Otero [87] mention that it is essential to take into account the temperature variation caused by pressure changes and the thermophysical properties of the products inside the pressure vessel, which cause different temperature increases after a pressure change. This variation can be determined using the following numerical Eq. (3):
where: T is the temperature \(\left(K\right)\), P is the pressure \(\left(Pa\right)\), V is the specific volume \(\left({m}^{3}/kg\right)\), \(\alpha\) is the thermal expansion coefficient \(\left({K}^{-1}\right)\) and \({c}_{p}\) is the specific heat capacity \(\left(\frac{J}{kg}\times K\right)\).
High-pressure freezing has been demonstrated to have favorable effects on the preservation of cell structure in food due to its ability to cause rapid nucleation leading to the uniform formation of microscopic ice crystals. This ensures that textural deteriorations to food products are minimized [90]. In peach and mango, high-pressure exchange freezing has been assessed for its microstructural effects. The product’s original structure was effectively preserved, and its textural quality remained almost intact. This technology considerably reduces the issues of freeze cracking or the creation of large ice crystals, which are frequently seen in conventional freezing [59]. The results are similar to those obtained in previous studies using this technology on vegetables such as carrots and Chinese cabbage (Table 2) [91, 92].
Microwave-Assisted Freezing
The use of microwave technology is a method that relies on an alternating electromagnetic field that produces thermal energy through the rotational movement of water molecules and ionic species. Since the frequency of microwaves typically ranges from 300 to 3000 MHz, this field causes rapid dipole movement in water and ionic/polar molecules [105]. In addition, heating occurs due to the deformation and friction of the water molecules, while the energy dissipated by the microwaves promotes the partial melting of formed ice crystals [18]. Two hypotheses have been proposed to explain this beneficial effect: (i) the microwave-induced (constant or pulsed) rotation of water molecules around crystals could disrupt ice crystal growth, as it impacts on hydrogen bonds between water molecules and water clusters during freezing and (ii) temperature oscillations due to increased microwave power could cause partial melting of crystals and lead to increased secondary nucleation. In both cases, this would lead to an increased number of smaller crystals, which were demonstrated by [106, 107], where a reduction of crystal size in food matrices in the order of 15-20% was evidenced.
Figure 2D shows the heating mechanisms of water molecules by (1) dipolar polarization and (2) ionic conduction triggered by microwave irradiation. Sadot, Curet, Le-Bail, Rouaud, and Havet [108] propose a mathematical model that is founded on an enthalpy formulation of the heat Eq. (4), incorporating terms deriving from Maxwell’s Eq. (5):
where: \(\omega\) is the pulsation (rad·s −1), \({\varepsilon }_{0}\) is the vacuum permittivity (F·m −1), \({\varepsilon }^{{\prime}{\prime}}\) is the relative dielectric loss factor, Q is the heat source term, (W·m −3), E is the local electric field, (V·m −1), \(\rho\) is the density (kg·m −3), H is the specific enthalpy, (kJ/kg), t is the time (s), k is the thermal conductivity, (W·m −1 ·K −1) and T is the temperature (K).
In freezing, the use of microwave technology enables efficient regulation of ice crystal formation within the cellular structure of food. This addresses a significant challenge often encountered during traditional freezing practices, positioning this method as a viable alternative for enhancing the freezing process [19]. The diminishment of crystal size is believed to arise from the rotation of water molecules caused by the alternating electric field produced by microwaves, which interferes with hydrogen bonds and may lead to the formation of the crystalline structure [109]. This is confirmed by a study that evaluated the effects of microwave-assisted freezing on potatoes and apples (Table 1). It was observed that freezing parameters including characteristic freezing time, overall freezing time and freezing rate, were not impacted in either of the products. The freezing curves of microwave-assisted freezing were very similar to those of conventional freezing. However, microwave-assisted freezing has less drip loss and consequently foods retain their firmness and texture [60].
Osmotic Dehydration-Assisted Freezing
The aim of dehydration is to produce foods with low moisture levels (aw below 0.70) or intermediate moisture (aw from 0.70 to 0.85) [50]. Osmotic processing consists of treating the food product with a hypertonic solution (low aw) to remove water from the food by an osmotic mechanism. Osmotic solutes of high and low molecular weight such as sugars (sucrose, honey, glucose, fructose, sorbitol, corn syrup), salts (sodium chloride, potassium chloride, calcium chloride), polyols (glycerol, sorbitol, erythritol), organic acids (lactate and ascorbic acid), concentrated fruit juices and combinations thereof are often used in an oxygen-free environment to avoid oxidative reactions [50]. Under these conditions, there is a bidirectional transfer of mass taking place: (i) water moves from the product to the osmotic solution and (ii) the osmotic solute is transferred from the solution to the product [110]. This mass transfer phenomenon is explained by selective osmotic transport through semi-permeable cell membranes [111], where water loss is greater than solute gain. Solutes enter the area between the cell membrane and the cell wall once they cross the membrane [112].
In addition, the leaching of soluble products (such as sugars, acids, minerals, and vitamins) has a significant impact on the sensory and nutritional properties of the product [113]. A combination of water and solute is then transported from the surface of the food product towards the center due to the osmotic pressure created between the osmotic solution and the food matrix. The process concludes when osmotic equilibrium is attained [114]. Mass transfer during the osmotic dehydration process of F&V is schematically presented in Fig. 2E. In general, this method is typically used as a pre-treatment before freezing food to reduce the amount of water present and, consequently, minimize crystal formation during the process. This process promotes an even distribution of crystals, which reduces damage to cell membranes [20] and results in significant benefits in the final product [115]. Such favorable outcomes are associated with the enhancement and maintenance of the inherent features of the food matrix, including sensory, nutritional, and functional attributes [116]. Additionally, this method is cost-effective as it employs basic equipment and consumes relatively low energy [70].
Goula and Lazarides [117] conducted numerical modelling to calculate water loss, solute gain, and the eventual equilibrium point following the process of osmotic dehydration. They obtained the Eqs. 6 y 7:
where: WL is the water loss, SG is the solid gain, \({s}_{1}\) and \({s}_{2}\) are parameters that can be defined as relative rate constants for water loss and solids gain, respectively, t is the time, \({WL}_{\alpha }\) is the water loss at equilibrium and \({SG}_{\alpha }\) is the solid gain at equilibrium. Eqs. 6 and 7 can be linearized to obtain the Eqs. 8 and 9 comprise the Azuara Model.
A study was conducted to examine the impact of osmotic pre-treatment and freezing on the volatile fraction of strawberries (Table 1). It was determined that using osmotic pre-treatment before freezing was advantageous, as it augmented the production of crucial aroma compounds in the fruit, thereby avoiding loss of quality [64]. Similarly, in the volatile fraction of kiwifruit, osmotic dehydration resulted in the creation of esters whilst decreasing the presence of aldehydes and alcohol [73]. A study investigated the effects of osmotic dehydration and freezing on papaya pieces. The findings were positive, as the brightness of the yellow color increased with the prolongation of osmotic dehydration. Recent studies have shown that this technology applied to the arazá fruit improved the freezing rate, avoided drip loss, which resulted in enhanced bioaccessibility of polyphenols with higher antioxidant capacity [71]. However, it is crucial to monitor the time factor as it could have an impact on the sweetness [65]. In a study conducted on apple cubes, it was noted that greater concentration of the osmotic medium resulted in higher firmness [74]. This observation was also supported by a similar study that found dehydro-freezing to effectively enhance the firmness of frozen products under examination, including peppers, carrots, and cucumber, while considerably reducing drip losses (Table 2) [12].
Unconventional Freezing Variants
Cryogenic Freezing
Ultra-rapid freezing, facilitated by cryogenic fluids, provides exceptionally high freezing rates. This process generates numerous small ice crystals in both intercellular and intracellular cavities and reduces water dislocation, thus avoiding detrimental effects caused by dehydration and shrinkage of the microstructure. As a result, the overall quality of the frozen food is effectively preserved. In addition, during cryogenic freezing, an outer layer or crust of ice forms on the surface of the product. This outer layer of ice gives the product mechanical strength and acts as a moisture barrier. The operational advantages of products frozen by this method are non-agglomeration and adherence to mechanical conveyor belts compared to other conventional freezing methods [75, 118].
This is based on the use of cryogenic liquids that allows a temperature reduction to occur quickly, the most used are CO2 and nitrogen gases [119]. Tangtua et al. [76] mention that these cryogenic liquids are responsible for the rapid absorption of heat, thus reducing the temperature of the food below its freezing point. Consequently, great benefits are obtained, among which we can mention the conservation of nutrients, organoleptic characteristics, and cellular structure, since the leakage of intracellular liquid produced after the defrosting of the food is reduced. In Fig. 3A, a cryogenic food freezer is graphically represented, in which nitrogen is being used as a cryogenic liquid.
Likewise, Tangtua et al. [76] evaluated the methodology to stop the activity of the polyphenol oxidase (PPO) and the peroxidase (POD) enzymes in ripe mango pulp before cryogenic freezing after storage, where the immersion of mango pulp in a citric acid/Calcium chloride solution and the immersion of litchi pulp in a calcium chloride solution improved fruit firmness, moreover, cryogenic freezing with liquid nitrogen were effective treatments to control enzymes and minimized changes in fruit quality during frozen storage at -24 °C for six months. On the other hand, Alhamdan et al. [77] mention that the liquid nitrogen cryogenic freezing (LNCF) method is superior to the conventional slow freezing method using the deep freezer (CSF) method during frozen storage in preserving the basic color parameters of fresh Barhi fruit and the deterioration of its texture, this due to the reduction of the harmful effects of crystallization and recrystallization in the microstructure of the F&V tissues during quick freezing method. Numerical model to analyze the heat transfer process in the freezing of myrtle, which will allow the establishment of a theoretical basis for subsequent experiments of this type of freezing in blueberries, having the Eq. (10):
where: “Re” is the Reynolds number, Nu is the Nusselt number, Pr is the Prandtl number, and η is the viscosity.
Supporting that, the quality of a frozen product depends on the temperature and speed of operation since they determine the distribution and size of ice crystals formed in the tissues. On the other hand, Razali et al. [21] observed that cryogenic freezing is indeed a promising method to preserve the quality of export fruits such as durian, since it positively prolongs storage life and also prevents durian dehiscence compared to conventional freezing (Table 1).
Isochoric Freezing
In isochoric freezing, food is submerged in an isotonic solution and then subjected to temperatures below 0°C. This freezing technique is characterized by being carried out under constant volume conditions. It is based on the principle of Le Chatelier also known as “The law of equilibrium”, which describes that as the formation of ice occurs and it expands, the pressure increases and, consequently, hinder the formation of ice inside the food matrix, which explains why part of the volume remains thawed or, in other words, in the liquid phase. The isochoric freezing sample represented in a phase diagram of pure water follows the trajectory of the liquid state curve, which lies at the interface between ice I, ice III and liquid water. This sample reaches an equilibrium pressure up to the triple point of 209.9 MPa at a sub-zero temperature of -21.985 °C, where a considerable part of the water contained in the sample remains liquid (45%). These process conditions restrict any further ice development and also prevent damage caused by the expansion of ice crystals during the transition phase of the water state [120].This increase in pressure will cease once a thermodynamic equilibrium is established between ice and water at the given freezing temperature [121]. Figure 3B graphically represents what was previously described.
Due to the above, this technique is currently gaining preponderance, being classified as an emerging technology with remarkable efficiency capable of completely preserving the cellular microstructure of the food. Likewise, its application does not imply damage due to the formation of ice since the ice crystals are distributed evenly and are also capable of minimizing microbial growth. For several years, this technique has been developed by Rubinsky and his group [23, 122, 123].
In addition to being an efficient freezing technique, it is economical due to it allows significant energy savings where approximately 35% less energy is needed compared to traditional freezing [124, 125]. The authors, in turn, introduced a numerical model that represents the freezing process in isochoric thermodynamic systems, having the following numerical equations that establish the relationship between the energy required to freeze a volume (V) and a certain temperature in an isochoric system concerning an isobaric system is given to the Stefan number (11):
and the isochoric frozen fraction IF = IP/100, for (12):
where: \(Ste\) is the number of de Stefan, \(c\) is the specific heat \((\frac{J}{kg}.K)\), \(T\) is the temperature \((K)\), \(init\) means initial, \(L\) represents the change of de enthalpy between frozen and thawed phases \((kJ/kg)\), \(R\) is the relation of energy \((\frac{W}{m}.K)\), \({\text{IF}}\) represents the isochoric frozen fraction, subscripts 1 and 2 represent the frozen and liquid domain respectively.
Regarding fruits, this technique has been applied in sweet cherry freezing performed by Bilbao-Sainz et al. [39] where it was observed that in an isochoric system under a temperature of -4 °C, the physical, nutritional, and organoleptic characteristics of the fruit were preserved, being these very similar to fresh cherries. Similarly, Bilbao-Sainz et al. [78] studied the effects on tomato conservation by applying this technique, where it was observed, that the preservation of the physical, nutritional, and organoleptic characteristics of the fruit, being very similar to the product in a fresh state. On the other hand, Bilbao-Sainz et al. [23] carried out a comparative study on the conservation of pomegranate through isochoric freezing and isochoric supercooling, the latter differs because during this process the food is brought well below its freezing point without the formation of ice crystals inside the food container. As a result, it was observed that in isochoric supercooling the physical, nutritional, and organoleptic properties of the product were maintained, likewise, the contents of ascorbic acid and anthocyanins increased. However, in isochoric freezing, although the physical and nutritional properties were preserved, in terms of organoleptic properties, the texture of the pomegranate arils was affected. In this sense, the isochoric supercooling technique turns out to have a greater efficiency for the preservation of the properties of freshly cut arils.
On the other hand, positive effects have also been found in potatoes and spinach, where isochoric freezing effectively leads to less loss due to dripping and volume contraction, as well as a better-preserved texture and microstructure [24, 104]. Even though this technique has been studied for several years, there is still not enough information to fully understand the mechanisms involved in conservation processes under isochoric conditions, which is why it is important to continue carrying out studies and research on this topic [126, 127].
Trends And Future Prospects
Over the years, new study approaches have emerged in the frozen F&V industry, as shown in Fig. 4, where the main studies that have been carried out for periods between 1955 and the present are observed. Initially from the years 1955–2000, the main approaches were sensory, instrumental analysis, and frozen storage, having the papaya greater research field. From 2001 to 2010, emphasis was placed on increasing the freezing speed and preserving the nutraceutical value of F&V, emerging rapid freezing methods (cryogenic freezing), and incorporating assisted technologies such as microwave. In the last decade, from 2011 to 2022, the studies had more emphasis on quality, frozen storage, microstructure, antioxidant activity/capacity, enzymatic activity, and optimization of the freezing process. Studies began on emerging combined technologies, such as ultrasound, osmotic dehydration, and dehydrofreezing.
On the other hand, it is important to mention that China is the country with the highest scientific production in food freezing with around 72 papers published in high impact journals (Fig. 5a). In addition, this country together with Thailand (8), India (7) and Japan (6) are the Asian territories that focus their research efforts on food freezing with a total number of 93 scientific publications, and it is this continent that leads the world. In addition, China has established direct collaborative networks with Russia and Canada (Fig. 5b), thereby increasing its scientific research and contributing to that of other countries in this regard. Similarly collaborative networks are established by countries such as France with India and Ukraine, as well as Thailand with Japan and the United States with Canada. In the Americas, the United States was the leading country in terms of number of publications (17), followed by Brazil (12) and Canada (8), and together they represented the American continent with a total of 37 scientific productions. Among the European countries, Spain had the highest number of scientific publications (14), followed by France (11) and Ukraine (8), for a total of 33 European scientific publications. It is hoped that the countries with the most research will establish collaborative networks with countries in the Caribbean, South America and Africa, which have a high potential for F&V production due to their tropical climates, and thus carry out in-depth studies on the main export and native crops, which are the axis of development in these continents, and at the same time, with the help of these new technologies, large F&V wastes can be avoided.
This review provides new approaches related to new technologies related to the freezing of F&V that have not been addressed in the course of the last 20 years, but that are being incorporated at an industrial level in the area of frozen foods, standing out among them the freezing assisted by magnetic field, high pressures, and unconventional technologies such as isochoric freezing. These technologies have shown promise for the optimization of the freezing process and the preservation of food quality (Table 3), however, according to the literature on emerging technologies such as ultrasound, further research is required to optimize the operating conditions and parameters for its application at an industrial scale.
Conclusions
Overall, novel freezing assisted technologies and unconventional freezing variants have been shown to have great potential in the frozen F&V industry in terms of mitigating structural damage (dripping and loss of firmness) caused during the conventional freezing process, as they allow to reduce the freezing rate and promote the formation of microscopic ice crystals with uniform distribution. They also contribute to preserve the morphological integrity of cellular tissues, thus maintaining the organoleptic, functional and/or nutraceutical quality of F&V. They can also inhibit enzyme activity (peroxidase and polyphenoloxidase) and cause the destruction of potentially pathogenic micro-organisms. Among the novel technologies, ultrasound, high pressure, and microwaves allow obtaining high quality frozen products through various mechanisms related to the distribution of water in the food matrix, without transferring foreign components to the food. However, they are expensive technologies, and their scale of operation is limited. Meanwhile, osmotic dehydration or dehydrofreezing and magnetic field freezing are the most accessible and economical technologies, as they do not require sophisticated equipment and expensive refrigerants to carry out the freezing process.
Regarding unconventional processes, isochoric freezing by volume-invariant mechanisms retains 45% of the water in liquid state, avoiding tissue damage caused by the ice crystals expansion and can reduce the energy required to freeze a food product by up to 65–70% compared to conventional isobaric freezing. In cryogenic freezing, due to its very high freezing rate, it prevents dehydration and shrinkage of tissue microstructure, and also forms a crust that protects against mechanical damage and acts as a moisture barrier, thus maintaining the texture, colour, flavours and microbiological safety of food frozen by this method. On the other hand, the numerical models presented in this article allow a mathematical understanding of each revised technology and important parameters to control in the freezing process such as temperature, freezing time, heat transfer, and mass. Undoubtedly, these new combined technologies mean a significant advance in the frozen food industry, however, it is important to continue research on these technologies to better understand their mechanisms of action and their relationship with the optimization of the F&V freezing process.
Data Availability
No datasets were generated or analysed during the current study.
References
Porat R, Lichter A, Terry LA, Harker R, Buzby J (2018) Postharvest losses of fruit and vegetables during retail and in consumers’ homes: Quantifications, causes, and means of prevention. Postharvest Biol Technol 139:135–149. https://doi.org/10.1016/j.postharvbio.2017.11.019
Brasil IM, Siddiqui MW (2018) Chapter 1 - postharvest quality of fruits and vegetables: An overview. In: Siddiqui MW (ed) Preharvest modulation of postharvest fruit and vegetable quality. Academic Press, pp 1–40
Da Silva DL, Silveira AS, Ronzoni AF, Hermes CJL (2022) Effect of freezing rate on the quality of frozen strawberries (Fragaria x ananassa). Int J Refrig 144:46–54. https://doi.org/10.1016/j.ijrefrig.2022.07.006
Khan MIH, Wellard RM, Nagy SA, Joardder MUH, Karim MA (2017) Experimental investigation of bound and free water transport process during drying of hygroscopic food material. Int J Therm Sci 117:266–273. https://doi.org/10.1016/j.ijthermalsci.2017.04.006
Chi Khang V, Le Dang T, Van Muoi N, Thanh Truc T (2022) the evaluation of freezing temperatures and ripeness levels on the quality characteristics of frozen pineapple fruits. J Microbiol Biotechnol Food Sci 12(3):e5439. https://doi.org/10.55251/jmbfs.5439
Prakobsang S, Pornchalermpong P (2018) Comparison of the effect of freezing on the quality of ‘nam dokmai” mango fruit. MATEC Web Conf. https://doi.org/10.1051/matecconf/201819203027
Vintilă M, Veringă D, Bogoescu M, Sorică C (2019) Research on freezing behaviour of’Augusta’and’Simultan’blueberry fruits grown in Romania. Acta Horticulturae 1242:779–784
Mowafy SG, Sabbah MA, Mostafa YS, Elansari AM (2020) Effect of freezing rate on the quality properties of Medjool dates at the tamr stage. J Food Process Preserv 44(12):e14938. https://doi.org/10.1111/jfpp.14938
Rindang A, Darmawati E, Hartulistiyoso E (2022) Numerical methods and its application in freezing process. IOP Conf Ser: Earth Environ Sci 1038(1):012077. https://doi.org/10.1088/1755-1315/1038/1/012077
Muthukumarappan K, Marella C, Sunkesula V (2019) Chapter 15 - Food freezing technology. In: Kutz M (ed) Handbook of farm, dairy and food machinery engineering, 3rd edn. Academic Press, pp 389–415
Neri L, Faieta M, Di Mattia C, Sacchetti G, Mastrocola D, Pittia P (2020) Antioxidant activity in frozen plant foods: effect of cryoprotectants, freezing process and frozen storage. Food 9(12):1886. https://doi.org/10.3390/foods9121886
Schudel S, Prawiranto K, Defraeye T (2021) Comparison of freezing and convective dehydrofreezing of vegetables for reducing cell damage. J Food Eng 293:110376. https://doi.org/10.1016/j.jfoodeng.2020.110376
Alabi KP, Zhu Z, Sun D-W (2020) Transport phenomena and their effect on microstructure of frozen fruits and vegetables. Trends Food Sci Technol 101:63–72. https://doi.org/10.1016/j.tifs.2020.04.016
Comandini P, Blanda G, Soto-Caballero MC, Sala V, Tylewicz U, Mujica-Paz H, ... Gallina Toschi T (2013) Effects of power ultrasound on immersion freezing parameters of potatoes. Innov Food Sci Emerg Technol 18:120–125. https://doi.org/10.1016/j.ifset.2013.01.009
Panayampadan AS, Shafiq Alam M, Aslam R, Kumar Gupta S, Kaur Sidhu G (2022) Effects of alternating magnetic field on freezing of minimally processed guava. LWT 163:113544. https://doi.org/10.1016/j.lwt.2022.113544
Norton T, Sun D-W (2008) Recent advances in the use of high pressure as an effective processing technique in the food industry. Food Bioprocess Technol 1(1):2–34. https://doi.org/10.1007/s11947-007-0007-0
Akkari E (2007) Modélisation et commande de la décongélation par micro-ondes. Doctoral dissertation, Nantes
Curet S (2008) Traitements micro-ondes et transferts de chaleur en milieu multiphasique. Doctoral dissertation, Nantes
Jackson TH, Ungan A, Critser JK, Gao D (1997) Novel microwave technology for cryopreservation of biomaterials by suppression of apparent ice formation. Cryobiology 34(4):363–372. https://doi.org/10.1006/cryo.1997.2016
Robbers M, Singh RP, Cunha LM (1997) Osmotic-convective dehydrofreezing process for drying kiwifruit. J Food Sci 62(5):1039–1042. https://doi.org/10.1111/j.1365-2621.1997.tb15033.x
Razali NA, Wan Ibrahim WMH, Safari S, Rosly NK, Hamzah FA, Wan Husin WMRI (2022) Cryogenic freezing preserves the quality of whole durian fruit for the export market. Food Res. https://doi.org/10.26656/fr.2017.6(3).428
Zhao Y, Ji W, Guo J, Chen L, Tian C, Wang Y, Wang J (2020) Numerical and experimental study on the quick freezing process of the bayberry. Food Bioprod Process 119:98–107. https://doi.org/10.1016/j.fbp.2019.10.013
Bilbao-Sainz C, Chiou B-S, Takeoka G, Williams T, Wood D, Powell-Palm MJ, ... McHugh T (2022) Isochoric freezing and isochoric supercooling as innovative postharvest technologies for pomegranate preservation. Postharvest Biol Technol 194:112072. https://doi.org/10.1016/j.postharvbio.2022.112072
Bilbao-Sainz C, Zhao Y, Takeoka G, Williams T, Wood D, Chiou B-S, ... McHugh T (2020) Effect of isochoric freezing on quality aspects of minimally processed potatoes. J Food Sci 85(9):2656–2664. https://doi.org/10.1111/1750-3841.15377
Thakur S, Jha B, Bhardwaj N, Singh A, Sawale PD, Kumar A (2022) Isochoric freezing of foods: A review of instrumentation, mechanism, physicochemical influence, and applications. J Food Process Preserv. https://doi.org/10.1111/jfpp.17113
Ojha KS, Kerry JP, Tiwari BK, O’Donnell C (2016) Freezing for food preservation. Reference module in food science. Elsevier. https://doi.org/10.1016/B978-0-08-100596-5.03108-5
De Michelis AJ (2015) Congelación de frutas, hortalizas, hongos, carnes y masas. Procedimientos hogareños y comerciales de pequeña escala. Editorial Instituto Nacional de Tecnología Agropecuaria (INTA). Retrieved from: https://www.argentina.gob.ar/inta
Reid DS (1997) Overview of physical/chemical aspects of freezing. In: Erickson MC, Hung Y-C (eds) Quality in frozen food. Springer, US, Boston, MA, pp 10–28
Arora R (2018) Mechanism of freeze-thaw injury and recovery: A cool retrospective and warming up to new ideas. Plant Sci 270:301–313. https://doi.org/10.1016/j.plantsci.2018.03.002
Bonat Celli G, Ghanem A, Su-Ling Brooks M (2016) Influence of freezing process and frozen storage on the quality of fruits and fruit products. Food Rev Intl 32(3):280–304. https://doi.org/10.1080/87559129.2015.1075212
Li M, Wang Y, Wei X, Wang Z, Wang C, Du X, ... Tang H (2023) Effects of pretreatment and freezing storage on the bioactive components and antioxidant activity of two kinds of celery after postharvest. Food Chem X 18:100655. https://doi.org/10.1016/j.fochx.2023.100655
Najafabadi NS, Sahari MA, Barzegar M, Esfahani ZH (2023) Effect of processing conditions (conventional heating, microwave, chilling, and freezing) on the stability of some bioactive compounds of jujube fruit. Appl Food Res 3(1):100293. https://doi.org/10.1016/j.afres.2023.100293
Sablani SS (2015) Chapter 18 - Freezing of fruits and impact on anthocyanins. In: Preedy V (ed) Processing and impact on active components in food. Academic Press, San Diego, pp 147–156
Tao S, Pan Y (2023) Reduced degradation of the cell wall polysaccharides maintains higher tissue integrity of papaya (Carica papaya L.) during chilling storage. Postharvest Biol Technol 204:112446. https://doi.org/10.1016/j.postharvbio.2023.112446
Šic Žlabur J, Duralija B, Emanović Z, Mikulec N, Voća S (2021) Specialized metabolites profile of strawberry fruit during flash-freezing. Paper presented at the IX International Strawberry Symposium 1309. https://doi.org/10.17660/ActaHortic.2021.1309.133
Pereira CG, Resende JVd (2020) Behavior of the effective heat transfer coefficient and global thermal resistance in freezing of fruit juice model solutions in cylindrical packages. Food Science and Technology 40(4):993–999. https://doi.org/10.1590/fst.29019
Pham QT (2014) Food freezing and thawing calculations. Springer, New York
López y Rojas H, Riveros Villa F, Bernardo Tello A, Pérez Solís M (2018) Aseguramiento de la calidad de carne de cuy (Cavia porcellus) envasado al vacío y conservación por congelación. Investigación Valdizana 10(4):153–160. https://revistas.unheval.edu.pe/index.php/riv/article/view/77
Bilbao-Sainz C, Sinrod A, Powell-Palm MJ, Dao L, Takeoka G, Williams T, ... McHugh T (2019) Preservation of sweet cherry by isochoric (constant volume) freezing. Innov Food Sci Emerg Technol 52:108–115. https://doi.org/10.1016/j.ifset.2018.10.016
Otero L, Pérez-Mateos M, Rodríguez AC, Sanz PD (2017) Electromagnetic freezing: Effects of weak oscillating magnetic fields on crab sticks. J Food Eng 200:87–94. https://doi.org/10.1016/j.jfoodeng.2016.12.018
Zhang P, Zhu Z, Sun D-W (2018) Using power ultrasound to accelerate food freezing processes: Effects on freezing efficiency and food microstructure. Crit Rev Food Sci Nutr 58(16):2842–2853. https://doi.org/10.1080/10408398.2018.1482528
Robles-Ozuna L, Ochoa-Martínez L (2012) Ultrasonido y sus aplicaciones en el procesamiento de alimentos. Revista Iberoamericana de Tecnología Postcosecha 13(2):109–122
Das K, Zhang M, Bhandari B, Chen H, Bai B, Roy MC (2022) Ultrasound generation and ultrasonic application on fresh food freezing: Effects on freezing parameters, physicochemical properties and final quality of frozen foods. Food Rev Int. https://doi.org/10.1080/87559129.2022.2027436
Wu J, Jia X, Fan K (2022) Recent advances in the improvement of freezing time and physicochemical quality of frozen fruits and vegetables by ultrasound application. Int J Food Sci Technol 57(6):3352–3360. https://doi.org/10.1111/ijfs.15744
Zhu Z, Chen Z, Zhou Q, Sun D-W, Chen H, Zhao Y, Pan H (2018) Freezing efficiency and quality attributes as affected by voids in plant tissues during ultrasound-assisted immersion freezing. Food Bioprocess Technol 11(9):1615–1626. https://doi.org/10.1007/s11947-018-2103-8
Cao X, Wang Z, Bai G, Zhu D, Lü C (2021) Effect of ultrasound on freezing characteristics of fruits with different porosities. Food Sci 42(7):128–133. https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-20200327-394
Nowak KW, Zielinska M, Waszkielis KM (2019) The effect of ultrasound and freezing/thawing treatment on the physical properties of blueberries. Food Sci Biotechnol 28(3):741–749. https://doi.org/10.1007/s10068-018-0528-5
Xu B-G, Zhang M, Bhandari B, Cheng X-F, Islam MN (2015) Effect of ultrasound-assisted freezing on the physico-chemical properties and volatile compounds of red radish. Ultrason Sonochem 27:316–324. https://doi.org/10.1016/j.ultsonch.2015.04.014
Fu X, Belwal T, Cravotto G, Luo Z (2020) Sono-physical and sono-chemical effects of ultrasound: Primary applications in extraction and freezing operations and influence on food components. Ultrason Sonochem 60:104726. https://doi.org/10.1016/j.ultsonch.2019.104726
González-Pérez JE, Ramírez-Corona N, López-Malo A (2021) Mass transfer during osmotic dehydration of fruits and vegetables: process factors and non-thermal methods. Food Eng Rev 13(2):344–374. https://doi.org/10.1007/s12393-020-09276-3
Otero L (2023) Application of high pressure processing in freezing and thawing processes. Non-thermal food processing operations. Elsevier, pp 359–405. https://doi.org/10.1016/B978-0-12-818717-3.00005-6
Zhao S, Wu J, Guo Z, Wang D, Chen J, Liu Q, ... Tao T (2024) Magnetic field technology in improving the quality of food refrigeration and freezing: Mechanisms, applications, and challenges. J Stored Prod Res 106:102254. https://doi.org/10.1016/j.jspr.2024.102254
Cheng X-F, Zhang M, Adhikari B, Islam MN, Xu B-G (2014) Effect of ultrasound irradiation on some freezing parameters of ultrasound-assisted immersion freezing of strawberries. Int J Refrig 44:49–55. https://doi.org/10.1016/j.ijrefrig.2014.04.017
Delgado AE, Zheng L, Sun D-W (2009) Influence of ultrasound on freezing rate of immersion-frozen apples. Food Bioprocess Technol 2(3):263–270. https://doi.org/10.1007/s11947-008-0111-9
Tang J, Shao S, Tian C (2020) Effects of the magnetic field on the freezing process of blueberry. Int J Refrig 113:288–295. https://doi.org/10.1016/j.ijrefrig.2019.12.022
Tang J, Zhang H, Tian C, Shao S (2020) Effects of different magnetic fields on the freezing parameters of cherry. J Food Eng 278:109949. https://doi.org/10.1016/j.jfoodeng.2020.109949
Yang Z, Zhang L, Zhao S, Luo N, Deng Q (2020) Comparison study of static and alternating magnetic field treatments on the quality preservation effect of cherry tomato at low temperature. J Food Process Eng 43(9):e13453. https://doi.org/10.1111/jfpe.13453
Leng D, Zhang H, Tian C, Li P, Kong F, Zhan B (2022) Static magnetic field assisted freezing of four kinds of fruits and vegetables: Micro and macro effects: Congélation assistée par champ magnétique statique de quatre types de fruits et légumes: micro et macro effets. Int J Refrig. https://doi.org/10.1016/j.ijrefrig.2022.10.018
Otero L, Martino M, Zaritzky N, Solas M, Sanz PD (2000) Preservation of microstructure in peach and mango during high-pressure-shift freezing. J Food Sci 65(3):466–470. https://doi.org/10.1111/j.1365-2621.2000.tb16029.x
Jha PK, Chevallier S, Xanthakis E, Jury V, Le-Bail A (2020) Effect of innovative microwave assisted freezing (MAF) on the quality attributes of apples and potatoes. Food Chem 309:125594. https://doi.org/10.1016/j.foodchem.2019.125594
Chen B-L, Lin G-S, Amani M, Yan W-M (2023) Microwave-assisted freeze drying of pineapple: kinetic, product quality, and energy consumption. Case Stud Therm Eng 41:102682. https://doi.org/10.1016/j.csite.2022.102682
Jiang H, Zhang M, Mujumdar AS (2010) Physico-chemical changes during different stages of MFD/FD banana chips. J Food Eng 101(2):140–145. https://doi.org/10.1016/j.jfoodeng.2010.06.002
Zielinska M, Sadowski P, Błaszczak W (2015) Freezing/thawing and microwave-assisted drying of blueberries (Vaccinium corymbosum L.). LWT Food Sci Technol 62(1, Part 2):555–563. https://doi.org/10.1016/j.lwt.2014.08.002
Talens P, Escriche I, Martinez-Navarrete N, Chiralt A (2002) Study of the influence of osmotic dehydration and freezing on the volatile profile of strawberries. J Food Sci 67(5):1648–1653. https://doi.org/10.1111/j.1365-2621.2002.tb08699.x
Moyano PC, Vega RE, Bunger A, Garretón J, Osorio FA (2002) Effect of combined processes of osmotic dehydration and freezing on papaya preservation. Food Sci Technol Int 8(5):295–301. https://doi.org/10.1106/10820130202918
Forni E, Sormani A, Scalise S, Torreggiani D (1997) The influence of sugar composition on the colour stability of osmodehydrofrozen intermediate moisture apricots. Food Res Int 30(2):87–94. https://doi.org/10.1016/S0963-9969(97)00038-0
Dermesonlouoglou EK, Pourgouri S, Taoukis PS (2008) Kinetic study of the effect of the osmotic dehydration pre-treatment to the shelf life of frozen cucumber. Innov Food Sci Emerg Technol 9(4):542–549. https://doi.org/10.1016/j.ifset.2008.01.002
Rincon A, Kerr WL (2010) Influence of osmotic dehydration, ripeness and frozen storage on physicochemical properties of mango. J Food Process Preserv 34(5):887–903. https://doi.org/10.1111/j.1745-4549.2009.00404.x
Agnelli ME, Marani CM, Mascheroni RH (2005) Modelling of heat and mass transfer during (osmo) dehydrofreezing of fruits. J Food Eng 69(4):415–424. https://doi.org/10.1016/j.jfoodeng.2004.08.034
Ramallo LA, Mascheroni RH (2010) Dehydrofreezing of pineapple. J Food Eng 99(3):269–275. https://doi.org/10.1016/j.jfoodeng.2010.02.026
Reyes-Alvarez CA, Lanari MC (2023) Effect of freezing, osmodehydro-freezing, freezedrying and osmodehydro-freezedrying on the physicochemical and nutritional properties of arazá (Eugenia stipitata McVaugh). Food Chemistry Advances 3:100496. https://doi.org/10.1016/j.focha.2023.100496
Lowithun N, Charoenrein S (2009) Influence of osmodehydrofreezing with different sugars on the quality of frozen rambutan. Int J Food Sci Technol 44(11):2183–2188. https://doi.org/10.1111/j.1365-2621.2009.02058.x
Talens P, Escriche I, Martı́nez-Navarrete N, Chiralt A (2003) Influence of osmotic dehydration and freezing on the volatile profile of kiwi fruit. Food Res Int 36(6):635–642. https://doi.org/10.1016/S0963-9969(03)00016-4
Bunger A, Moyano PC, Vega RE, Guerrero P, Osorio F (2004) osmotic dehydration and freezing as combined processes on apple preservation. Food Sci Technol Int 10(3):163–170. https://doi.org/10.1177/1082013204044828
Li J, Chotiko A, Kyereh E, Zhang J, Liu C, Ortega VVR, ... Sathivel S (2017) Development of a combined osmotic dehydration and cryogenic freezing process for minimizing quality changes during freezing with application to fruits and vegetables. J Food Process Preserv 41(1):e12926. https://doi.org/10.1111/jfpp.12926
Tangtua J, Leksawasdi N, Rattanapanone N (2014) Quality changes in ripened mango and litchi flesh after cryogenic freezing and during storage. Chiang Mai Univ J Nat Sci. https://doi.org/10.12982/cmujns.2014.0036
Alhamdan A, Hassan B, Alkahtani H, Abdelkarim D, Younis M (2018) Cryogenic freezing of fresh date fruits for quality preservation during frozen storage. J Saudi Soc Agric Sci 17(1):9–16. https://doi.org/10.1016/j.jssas.2015.12.001
Bilbao-Sainz C, Sinrod AJG, Dao L, Takeoka G, Williams T, Wood D, ... McHugh T (2021) Preservation of grape tomato by isochoric freezing. Food Res Int 143:110228. https://doi.org/10.1016/j.foodres.2021.110228
Cong J, Gao P, Liu X, Wang Y, Liu M (2021) Droplet freezing phase transition and heat transfer under the ultrasonic effect. Int Commun Heat Mass Transfer 123:105136. https://doi.org/10.1016/j.icheatmasstransfer.2021.105136
Yu H, Mei J, Xie J (2022) New ultrasonic assisted technology of freezing, cooling and thawing in solid food processing: A review. Ultrason Sonochem 90:106185. https://doi.org/10.1016/j.ultsonch.2022.106185
Jiang Q, Zhang M, Mujumdar AS, Qu P, Hu R (2022) Pressurized carbon dioxide combined with ultrasound-assisted immersion freezing: Effects on microstructure and nucleation of honeydew melon. Int J Refrig 137:212–219. https://doi.org/10.1016/j.ijrefrig.2022.01.031
Kaur M, Kumar M (2020) An innovation in magnetic field assisted freezing of perishable fruits and vegetables: A review. Food Rev Intl 36(8):761–780. https://doi.org/10.1080/87559129.2019.1683746
Li D, Zhu Z, Sun D-W (2018) Effects of freezing on cell structure of fresh cellular food materials: A review. Trends Food Sci Technol 75:46–55. https://doi.org/10.1016/j.tifs.2018.02.019
Kaur M, Kumar M, Sethi V (2022) Maintaining the freeze thawing characteristics of tomato through development and evaluation of magnetic field-assisted freezing system. J Food Process Preserv 46(9):e16900. https://doi.org/10.1111/jfpp.16900
Jiang Q, Zhang M, Mujumdar AS, Chen B (2022) Comparative freezing study of broccoli and cauliflower: Effects of electrostatic field and static magnetic field. Food Chem 397:133751. https://doi.org/10.1016/j.foodchem.2022.133751
Li T, An G, Sun Q, Xu T, Du D, Zhang Y, ... Xia G (2023) Effects of high voltage electrostatic field and weak magnetic field assisted refrigeration on preservation of spinach. J Food Meas Charact 17(6):6484–6502. https://doi.org/10.1007/s11694-023-02119-9
Sanz PD, Otero L (2014) Chapter 28 - High-pressure freezing. In: Sun D-W (ed) Emerging technologies for food processing, 2nd edn. Academic Press, San Diego, pp 515–538
Jiang Q, Zhang M, Mujumdar AS (2023) Application of physical field-assisted freezing and thawing to mitigate damage to frozen food. J Sci Food Agric 103(5):2223–2238. https://doi.org/10.1002/jsfa.12260
Cheng L, Zhu Z, Sun D-W (2021) Impacts of high pressure assisted freezing on the denaturation of polyphenol oxidase. Food Chem 335:127485. https://doi.org/10.1016/j.foodchem.2020.127485
Van Buggenhout S, Messagie I, Van der Plancken I, Hendrickx M (2006) Influence of high-pressure–low-temperature treatments on fruit and vegetable quality related enzymes. Eur Food Res Technol 223(4):475–485. https://doi.org/10.1007/s00217-005-0227-3
Fuchigami M, Kato N, Teramoto AI (1998) High-pressure-freezing effects on textural quality of chinese cabbage. J Food Sci 63(1):122–125. https://doi.org/10.1111/j.1365-2621.1998.tb15690.x
Fuchigami M, Kato N, Teramoto AI (1997) high-pressure-freezing effects on textural quality of carrots. J Food Sci 62(4):804–808. https://doi.org/10.1111/j.1365-2621.1997.tb15459.x
Xin Y, Zhang M, Adhikari B (2014) Ultrasound assisted immersion freezing of broccoli (Brassica oleracea L. var. botrytis L.). Ultrason Sonochem 21(5):1728–1735. https://doi.org/10.1016/j.ultsonch.2014.03.017
Liu B, Song J, Yao Z, Bennacer R (2017) Effects of magnetic field on the phase change cells and the formation of ice crystals in biomaterials: carrot case. J Therm Sci Eng Appl 9(3):031005. https://doi.org/10.1115/1.4035936
Otero L, Pozo A (2022) Effects of the application of static magnetic fields during potato freezing. J Food Eng 316:110838. https://doi.org/10.1016/j.jfoodeng.2021.110838
Koch H, Seyderhelm I, Wille P, Kalichevsky MT, Knorr D (1996) Pressure-shift freezing and its influence on texture, colour, microstructure and rehydration behaviour of potato cubes. Food/Nahrung 40(3):125–131. https://doi.org/10.1002/food.19960400306
Otero L, Solas MT, Sanz PD, de Elvira C, Carrasco JA (1998) Contrasting effects of high-pressure-assisted freezing and conventional air-freezing on eggplant tissue microstructure. Z Lebensm-Unters-Forsch 206(5):338–342. https://doi.org/10.1007/s002170050269
Duan X, Zhang M, Mujumdar AS (2007) Studies on the microwave freeze drying technique and sterilization characteristics of cabbage. Dry Technol 25(10):1725–1731. https://doi.org/10.1080/07373930701591044
Yan W-Q, Zhang M, Huang L-L, Tang J, Mujumdar AS, Sun J-C (2010) Studies on different combined microwave drying of carrot pieces. Int J Food Sci Technol 45(10):2141–2148. https://doi.org/10.1111/j.1365-2621.2010.02380.x
Ohnishi S, Miyawaki O (2005) Osmotic dehydrofreezing for protection of rheological properties of agricultural products from freezing-injury. Food Sci Technol Res 11(1):52–58. https://doi.org/10.3136/fstr.11.52
Giannakourou MC, Taoukis PS (2003) Stability of dehydrofrozen green peas pretreated with nonconventional osmotic agents. J Food Sci 68(6):2002–2010. https://doi.org/10.1111/j.1365-2621.2003.tb07009.x
Kidmose U, Martens HJ (1999) Changes in texture, microstructure and nutritional quality of carrot slices during blanching and freezing. J Sci Food Agric 79(12):1747–1753. https://doi.org/10.1002/(SICI)1097-0010(199909)79:12%3C1747::AID-JSFA429%3E3.0.CO;2-B
Kidmose U, Kaack K (1999) Changes in texture and nutritional quality of green asparagus spears (Asparagus officinalis L.) during microwave blanching and cryogenic freezing. Acta Agric Scand Sect B 49(2):110–116. https://doi.org/10.1080/09064719950135623
Bilbao-Sainz C, Sinrod AGJ, Dao L, Takeoka G, Williams T, Wood D, ... McHugh T (2020) Preservation of spinach by isochoric (constant volume) freezing. Int J Food Sci Technol 55(5):2141–2151. https://doi.org/10.1111/ijfs.14463
Xin Y, Zhang M, Xu B, Adhikari B, Sun J (2015) Research trends in selected blanching pretreatments and quick freezing technologies as applied in fruits and vegetables: A review. Int J Refrig 57:11–25. https://doi.org/10.1016/j.ijrefrig.2015.04.015
Havet M, Sadot M, Jha P, Chevallier S, Jury V, Curet S, Rouaud O, Le-Bail A (2019) Congélation de produits alimentaires: amélioration de la cristallisation par applications de micro-ondes. IAA. Industries alimentaires et agricoles
Köprüalan Aydın Ö, Yüksel Sarıoğlu H, Dirim SN, Kaymak-Ertekin F (2023) Recent advances for rapid freezing and thawing methods of foods. Food Eng Rev 15(4):667–690. https://doi.org/10.1007/s12393-023-09356-0
Sadot M, Curet S, Le-Bail A, Rouaud O, Havet M (2020) Microwave assisted freezing part 1: Experimental investigation and numerical modeling. Innovate Food Sci Emerg Technol 62:102360. https://doi.org/10.1016/j.ifset.2020.102360
Sadot M, Curet S, Chevallier S, Le-Bail A, Rouaud O, Havet M (2020) Microwave assisted freezing part 2: Impact of microwave energy and duty cycle on ice crystal size distribution. Innovate Food Sci Emerg Technol 62:102359. https://doi.org/10.1016/j.ifset.2020.102359
Lazarides HN, Gekas V, Mavroudis N (1997) Apparent mass diffusivities in fruit and vegetable tissues undergoing osmotic processing. J Food Eng 31(3):315–324. https://doi.org/10.1016/S0260-8774(96)00084-2
Spiazzi E, Mascheroni R (1997) Mass transfer model for osmotic dehydration of fruits and vegetables—I. Development of the simulation model. J Food Eng 34(4):387–410. https://doi.org/10.1016/S0260-8774(97)00102-7
Dash KK, Balasubramaniam VM, Kamat S (2019) High pressure assisted osmotic dehydrated ginger slices. J Food Eng 247:19–29. https://doi.org/10.1016/j.jfoodeng.2018.11.024
Dixon GM, Jen JJ (1977) Changes of sugars and acids of osmovac-dried apple slices. J Food Sci 42(4):1126–1127. https://doi.org/10.1111/j.1365-2621.1977.tb12684.x
Phisut N (2012) Factors affecting mass transfer during osmotic dehydration of fruits. Int Food Res J 19(1):7
Forni E, Torreggiani D, Crivelli G, Maestrelli A, Bertolo G, Santelli F (1990) Influence of osmosis time on the quality of dehydrofrozen kiwi fruit. Acta Hortic 282:425–434. https://doi.org/10.17660/ActaHortic.1990.282.54. I International Symposium on Kiwifruit
Torreggiani D (1993) Osmotic dehydration in fruit and vegetable processing. Food Res Int 26(1):59–68. https://doi.org/10.1016/0963-9969(93)90106-S
Goula AM, Lazarides HN (2012) Modeling of mass and heat transfer during combined processes of osmotic dehydration and freezing (Osmo-Dehydro-Freezing). Chem Eng Sci 82:52–61. https://doi.org/10.1016/j.ces.2012.07.023
Jha PK, Chapleau N, Meyers P-E, Pathier D, Le-Bail A (2024) Can cryogenic freezing preserve the quality of fruit matrices during long-term storage compared to the mechanical method? Appl Food Res 4(1):100374. https://doi.org/10.1016/j.afres.2023.100374
de Sena M, Aquino AC, Soares Móes R, Almeida Castro A (2011) Estabilidade de ácido ascórbico, carotenoides e antocianinas de frutos de acerola congelados por métodos criogênicos. Braz J Food Technol 14(02):154–163. https://doi.org/10.4260/bjft2011140200020
Nida S, Moses JA, Anandharamakrishnan C (2021) Isochoric freezing and its emerging applications in food preservation. Food Eng Rev 13(4):812–821. https://doi.org/10.1007/s12393-021-09284-x
Rubinsky B, Perez PA, Carlson ME (2005) The thermodynamic principles of isochoric cryopreservation. Cryobiology 50(2):121–138. https://doi.org/10.1016/j.cryobiol.2004.12.002
Consiglio AN, Lilley D, Prasher R, Rubinsky B, Powell-Palm MJ (2022) Methods to stabilize aqueous supercooling identified by use of an isochoric nucleation detection (INDe) device. Cryobiology 106:91–101. https://doi.org/10.1016/j.cryobiol.2022.03.003
Powell-Palm MJ, Koh-Bell A, Rubinsky B (2020) Isochoric conditions enhance stability of metastable supercooled water. Appl Phys Lett 116(12):123702. https://doi.org/10.1063/1.5145334
Dhanya R, Panoth A, Venkatachalapathy N (2023) Comprehensive review on isochoric freezing: a recent technology for preservation of food and non-food items. Sustain Food Technol. https://doi.org/10.1039/D3FB00146F
Năstase G, Perez PA, Șerban A, Dobrovicescu A, Ștefănescu M-F, Rubinsky B (2016) Advantages of isochoric freezing for food preservation: A preliminary analysis. Int Commun Heat Mass Transfer 78:95–100. https://doi.org/10.1016/j.icheatmasstransfer.2016.08.026
Consiglio AN, Rubinsky B, Powell-Palm MJ (2022) Relating metabolism suppression and nucleation probability during supercooled biopreservation. J Biomech Eng. https://doi.org/10.1115/1.4054217
Powell-Palm M, Rubinsky B, Sun W (2020) Freezing water at constant volume and under confinement. Commun Phys 3(1):39. https://doi.org/10.1038/s42005-020-0303-9
Wu Z, Ma W, Xian Z, Liu Q, Hui A, Zhang W (2021) The impact of quick-freezing methods on the quality, moisture distribution and microstructure of prepared ground pork during storage duration. Ultrason Sonochem 78:105707. https://doi.org/10.1016/j.ultsonch.2021.105707
James C, Purnell G, James SJ (2014) A critical review of dehydrofreezing of fruits and vegetables. Food Bioprocess Technol 7(5):1219–1234. https://doi.org/10.1007/s11947-014-1293-y
Acknowledgements
Gratitude to the authorities of the Academic Programme of Agro-industrial Engineering of the Universidad Privada San Juan Bautista for providing the facilities to carry out this review work.
Funding
This research project did not receive funding.
Ethics declarations
Ethical Approval
In this systematic review article, no living organisms were used to collect or obtain experimental data.
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Loayza-Salazar, S., Siche, R., Vegas, C. et al. Novel Technologies in the Freezing Process and Their Impact on the Quality of Fruits and Vegetables. Food Eng Rev 16, 371–395 (2024). https://doi.org/10.1007/s12393-024-09371-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12393-024-09371-9