Abstract
CRYPTOCHROME-INTERACTING basic helix-loop-helix 1 (CIB1) is a well characterized transcriptional factor which promotes flowering through the physical interaction with the blue light receptor CRYPTOCHROME 2 (CRY2) in Arabidopsis. However, the role of its counterpart in crop species remains largely unknown. Here, we describe the isolation and characterization of a CIB1 homolog gene, Glycine max CIB1-LIKE10 (GmCIL10), from soybean genome. The mRNA expression of GmCIL10 in the unifoliate leaves shows a diunal rhythm in both long day (LD) and short day (SD) photoperiod, but it only oscillates with a circadian rhythm when the soybean is grown under LDs, indicating that the clock regulation of GmCIL10 transcription is LD photoperiod-dependent. Moreover, its mRNA expression varies in different tissue or organs, influenced by the develpomental stage, implying that GmCIL10 may be involved in the regulation of multiple developmental processes. Similar to CIB1, GmCIL10 was evident to be a nuclei protein and ectopically expression of GmCIL10 in transgenic Arabidopsis accelerates flowering under both LDs and SDs, implying that CIBs dependent regulation of flowering time is an evolutionarily conserved mechanism in different plant species.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cashmore AR. Cryptochromes: Enabling Plants and Animals to Determine Circadian Time. Cell, 2003, 114: 537–543
Sancar A. Structure and Function of DNA Photolyase and Crypto chrome Blue-Light Photoreceptors. Chem rev, 2003, 103: 2203–2238
Lin C, Shalitin D. Cryptochrome structure and signal transduction. Annu revplant biol, 2003, 54: 469–496
Liu B, Zuo Z, Liu H, Liu X, Lin C. Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Genes Dev, 2011, 25: 1029–1034
Ahmad M, Cashmore AR. HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature, 1993, 366: 162–166
Guo H, Yang H, Mockler TC, Lin C. Regulation of flowering time by Arabidopsis photoreceptors. Science, 1998, 279: 1360–1363
Liu H, Yu X, Li K, Klejnot J, Yang H, Lisiero D, Lin C. Photoexcited CRY2 Interacts with CIB1 to Regulate Transcription and Floral Initiation in Arabidopsis. Science, 2008, 322: 1535–1539
Ikeda M, Fujiwara S, Mitsuda N, Ohme-Takagi M. A triantagonistic basic helix-loop-helix system regulates cell elongation in Arabidopsis. Plant Cell, 2012, 24: 4483–4497
Liu Y, Li X, Li K, Liu H, Lin C. Multiple bHLH Proteins form Heterodimers to Mediate CRY2-Dependent Regulation of Flowering-Time in Arabidopsis. PLoS Genet, 2013, 9: e1003861
Atchley WR, Fitch WM. A natural classification of the basic helix-loop-helix class of transcription factors. Proc Natl Acad Sci USA, 1997, 94: 5172–5176
Littlewood TD, Evan GI. Helix-loop-helix transcription factors. Oxford University Press New York, 1998.
Ledent V, Vervoort M. The basic helix-loop-helix protein family: comparative genomics and phylogenetic analysis. Genome Res, 2001, 11: 754–770
Toledo-Ortiz G, Huq E, Quail PH. The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003, 15: 1749–1770
Nair SK, Burley SK. Functional genomics: recognizing DNA in the library. Nature, 2000, 404: 715–718
Murre C, McCaw PS, Baltimore D. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell, 1989, 56: 777–783
Ferre-D’Amare A, Pognonec P, Roeder R, Burley S. Structure and function of the b/HLH/Z domain of USF. EMBO J, 1994, 13: 180
Meng Y, Li H, Wang Q, Liu B, Lin C. Blue Light-Dependent Interaction between Cryptochrome2 and CIB1 Regulates Transcription and Leaf Senescence in Soybean. Plant Cell, 2013, 25: 4405–4420
Liu H, Liu B, Zhao C, Pepper M, Lin C. The action mechanisms of plant cryptochromes. Trends Plant Sci, 2011, 16: 684–691
Xue Z, Zhang X, Lei C, Chen X, Fu Y. Molecular cloning and functional analysis of one ZEITLUPE homolog GmZTL3 in soybean. Mol Biol Rep, 2012, 39: 1411–1418
Wu F, Zhang X, Li D, Fu Y. Ectopic Expression Reveals a Conserved PHYB Homolog in Soybean. PLoS ONE, 2011, 6: e27737
Huang G, Ma J, Han Y, Chen X, Fu Y. Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9. Plant Mol Biol Rep, 2011, 29: 352–359
Zhang Q, Li H, Li R, Hu R, Fan C, Chen F, Wang Z, Liu X, Fu Y, Lin C. Association of the circadian rhythmic expression of GmCRY1a with a latitudinal cline in photoperiodic flowering of soybean. PNAS U S A, 2008, 105: 21028–21033
Fan C, Wang X, Wang Y, Hu R, Zhang X, Chen J, Fu Y. Genome-Wide Expression Analysis of Soybean MADS Genes Showing Potential Function in the Seed Development. PLoS ONE, 2013, 8: e62288
Chen Q, Zhou H, Chen J, Wang X. Using a modified TA cloning method to create entry clones. Anal Biochem, 2006, 358: 120–125
Fan C, Wang X, Hu R, Wang Y, Xiao C, Jiang Y, Zhang X, Zheng C, Fu Y. The pattern of Phosphate transporter 1 genes evolutionary divergence in Glycine max L. BMC Plant Biol, 2013, 13: 48
Xiao C, Chen F, Yu X, Lin C, Fu Y. Over-expression of an AT-hook gene, AHL22, delays flowering and inhibits the elongation of the hypocotyl in Arabidopsis thaliana. Plant Mol Biol, 2009, 71: 39–50
Yoo S, Cho Y, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc, 2007, 2: 1565–1572
Kong F, Liu B, Xia Z, Sato S, Kim BM, Watanabe S, Yamada T, Tabata S, Kanazawa A, Harada K. Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in soybean. Plant Physiol, 2010, 154: 1220–1231
Hu R, Fan C, Li H, Zhang Q, Fu Y. Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR. BMC Mol Biol, 2009, 10: 93
Author information
Authors and Affiliations
Corresponding authors
Additional information
Contributed equally to this work
This article is published with open access at springerlink.bibliotecabuap.elogim.com
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Yang, D., Zhao, W., Meng, Y. et al. A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci. China Life Sci. 58, 261–269 (2015). https://doi.org/10.1007/s11427-015-4815-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-015-4815-6