Abstract
Trait-based classifications can efficiently capture species’ responses to environmental gradients and their impacts on ecosystem functioning. Thus, the clustering of phytoplankton species into functional groups can improve the understanding of their relationships with the environment and help to predict their response to environmental changes. Accordingly, this study aimed to create habitat templates of Reynolds phytoplankton functional groups (RFGs) in tropical drinking water reservoirs to describe, explain, and predict their occurrence and formation of blooms. We analyzed the structure of RFGs in 10 tropical reservoirs, in humid and semiarid regions of Brazil, and defined their relationships with 10 environmental variables. We designated the habitat template based on niche differentiation, thresholds for the occurrences and bloom formation, cluster analyses, and generalized additive models. We identified 136 species, assembled in 20 RFGs. Six groups of habitat templates were recognized based on environmental conditions and dominant RFGs, usually represented by bloom-forming species of cyanobacteria, dinoflagellates, green algae, and diatoms. The functional groups D, X1, and P presented the most restrictive occurrences, while RFGs M and SN displayed the widest, occurring in almost all sets of conditions. Moreover, salinity was the best predictor of RFGs’ biomass (higher R2), followed by depth, soluble reactive phosphorus, irradiance, water transparency, and dissolved inorganic nitrogen. Our approach improves the understanding of how RFGs interact with environmental gradients in tropical reservoirs, helping water managers to adopt sustainable practices to control algal blooms, based on predictions of the future state of dominance.
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Introduction
Organisms’ traits can represent their response to environmental drivers or their effects on ecosystem functioning, besides increasing the predictability of the community’s response to environmental changes (Kruk et al., 2021). Thus, explaining the main processes driving plankton diversity and structure is a central question in community ecology (Hutchinson, 1961; Rojo, 2021). For phytoplankton assemblages, species coexistence is driven by several biotic and abiotic filters that affect species interactions (Borics et al., 2020). These filters influence more traits of species than species themselves, generating patterns of dominance when conditions become favourable to some species or not suitable for others (Reynolds et al., 2002).
Considering that phytoplankton is a highly diverse group of photosynthetic organisms (Borics et al., 2021), species-level classifications can often generate chaotic dynamics under certain circumstances, even after the advances in the study of physiology and ecology of the organisms (Benicà et al., 2015). To alleviate this problem, trait-based approaches and functional groups are often used to classify phytoplankton species without losing important features and responses (Reynolds et al., 2002; Salmaso & Pádisak, 2007; Kruk et al., 2010). The definition of functional groups can cluster different sets of species with common functional features that respond in a similar way to environmental changes and present the same effects on ecosystem functioning (Kruk et al., 2021).
Based on that, Reynolds (1998) and Reynolds et al. (2002) created phytoplankton functional groups (hereafter Reynolds Functional Groups – RFG, Kruk et al., 2017) to cluster species with similar tolerance and preferences and that co-occur in the same environment at the same time. These groups were created based on the habitat template, which included eight environmental variables: mixing zone depth, irradiance, water temperature, acidity and alkalinity, herbivorous zooplankton, soluble reactive phosphorus, dissolved inorganic nitrogen, and soluble reactive silica (Reynolds, 2006). Although this approach has been widely used for many years (Padisák et al., 2009; Kruk et al., 2021) and environmental conditions have been proven to be coherent with the assemblages (Salmaso et al., 2015), some groups are not well-defined in terms of habitat template and driving processes (Padisák et al., 2009), and some new environmental factors should be further studied, e.g., salinity and the amount of the inoculum (Kruk et al., 2021).
The diversity and composition of phytoplankton are primarily controlled by local filters and biotic interactions (Borics et al., 2021). Important filters select specific attributes that act more strongly in some species or groups, creating different patterns of abundance in response to the environmental gradients (Reynolds, 1998). As predicted by the “habitat template” concept, introduced by Southwood (1977), the environment exerts influence on the fitness of the organisms, populations, or groups of species, which will select certain adaptations for better reproduction and survival (Townsend et al., 1997). This approach has been proved to be useful for classifying and predicting phytoplankton community structure without losing important information on tolerance and requirements in the natural environment (Reynolds, 1998), both for RFGs (Reynolds, 2006) and for morphology-based functional groups (Kruk & Segura, 2012).
Answering the question “what lives where and why?” (Reynolds, 1998) has been a big challenge for phytoplankton ecologists over the years. Since the proposal of classification of RFGs, much has been done towards its development, especially to describe spatial–temporal patterns in phytoplankton (“what lives where?”), but less often to explain those patterns (“why and how?”), and scarcely to predict (“when and where in new scenarios”) (Kruk et al., 2021). To answer the question “what lives where”, a descriptive framework aiming to describe the quantitative habitat templates where species are distributed in space and time can be followed (Reynolds, 1998). When the focus is to answer “why and how”, ecologists must explain the main mechanisms driving community assembly, relationships with environmental variables, biological interactions, and ecosystem functioning. Finally, to understand “when and where in new scenarios” the focus should be on predicting future scenarios of community responses to environmental changes and effects on ecosystem functioning and services (Kruk et al., 2021).
Phytoplankton functional groups are often used in water quality assessments and algal bloom status in temperate, subtropical, and tropical lakes (e.g., Padisák et al., 2006; Crossetti et al., 2019; Braga & Becker, 2020; Long et al., 2020). Indeed, harmful algal blooms are among the main causes of biodiversity loss and the reduction of ecosystem functioning in freshwaters (Moura et al., 2018; Reid et al., 2019; Amorim & Moura, 2021). Consequently, phytoplankton-dominated shallow lakes support fewer ecosystem services than macrophyte-dominated systems, which can impair the achievement of the United Nations Sustainable Development Goals (Janssen et al., 2021). Therefore, understanding how phytoplankton assemblages interact with environmental gradients will help water managers to improve the sustainable use of drinking water reservoirs, ensuring the provision of food, clean water and sanitation, biodiversity, climate regulation, and sustainable development.
Accordingly, in this study, we evaluated the dynamics of RFGs in a set of tropical drinking water reservoirs and created links to the relevant environmental factors driving their biomass, distribution, and bloom development. Our main goal is to establish the main RFGs and their habitat templates for tropical reservoirs, to explain and predict their presence, and even blooms, in the foreseeable new conditions. Furthermore, our approach will help water managers to predict the main impacts of algal blooms on biodiversity, water quality, ecosystem functioning, and services, besides supporting the adoption of sustainable practices.
Material and methods
Study sites, sampling, and analyses
We sampled phytoplankton from 10 tropical drinking water reservoirs in Pernambuco state, Brazil. The reservoirs presented contrasting environmental conditions, ranging from oligotrophic to hypereutrophic, and are distributed in a climatic gradient, in the humid, dry-subhumid, and semiarid zones of Brazil (Table S1). Moreover, all of them presented algal blooms at least in one sampling period (Amorim et al., 2020; Amorim & Moura, 2021). All studied reservoirs provide several ecosystem services for people, supporting the water public supply, irrigation, agriculture, fisheries, livestock, biodiversity, recreation, education, flood control, nutrient retention, primary production, climate regulation, among others.
Samples were collected five (Tapacurá and Cajueiro reservoirs) or four (other reservoirs) times a year from October 2017 to January 2019 (n = 42), comprising an annual cycle in each reservoir, with samples every three months in the rainy and dry seasons. All samples were collected from the deepest location of each reservoir, usually close to the dam and during the morning (08:00–12:00). Water transparency and depth (Zmax) were measured with a Secchi disk and an echo sounder, respectively. Water temperature, salinity, and pH were measured with a HANNA multiparametric probe (HI98194). Water temperature was further verified throughout the water column to establish the mixing zone depth (Zmix) (when there was a difference in the water temperature greater than 0.5 °C at each 0.5 m depth, i.e., the formation of the thermocline in stratified environments). Destratified environments presented a mixing zone depth equal to or almost reaching the sediment, demonstrating a complete mixture of the water column (Zmix:Zmax ≈ 1). The dissolved inorganic nitrogen (DIN) was calculated based on the sum of ammonia, nitrate, and nitrite, which were determined by the reaction with sodium hypochlorite and phenol for ammonia and cadmium reduction for nitrate and nitrite. The soluble reactive phosphorus (SRP) was analyzed by the ammonium molybdate and ascorbic acid reduction, catalyzed by antimony ions. The analyses were performed according to Strickland & Parsons (1965), Golterman et al. (1971), and Valderrama (1981).
Samples collected through surface trawls with a 25 µm mesh-size plankton net, fixed with 4% formaldehyde, were used for phytoplankton species identification following specialized literature (Prescott & Vinyard, 1982; Komárek & Fott, 1983; Komárek & Anagnostidis, 1986, 2005; Anagnostidis & Komárek, 1988; Round et al., 1990; Krammer & Lange-Berlalot, 1991; John et al., 2002; Wehr et al., 2015; among others). Subsurface samples (20 cm depth) were collected directly from the water column, preserved with 1% acetic Lugol, and analyzed in sedimentation chambers using an inverted microscope for phytoplankton quantification (Utermöhl, 1958). Phytoplankton was counted until achieving 400 organisms of the most abundant taxa and until the stabilization of the species curve (i.e., when no species was added) (Lund et al., 1958). The species’ biomass (mg l−1, wet weight) was determined according to Hillebrand et al. (1999) and grouped into Reynolds Functional Groups (RFGs) (Reynolds et al., 2002; Padisak et al., 2009; Kruk et al., 2017; among others). All populations, even the rarest ones, were considered and classified into RFGs, following the recommendations of Rojo (2021).
Zooplankton was collected by filtering 100 l of water from the surface of the reservoirs with a 60 µm mesh-size plankton net; then the samples were fixed with 4% formaldehyde and analyzed in a 1 mL Sedgewick-Rafter chamber (three subsamples per sample or the entire sample) in an optical microscope. Biomass (µg l−1, dry weight) was calculated based on regression formulas relating the bodyweight with length and width of rotifers and microcrustaceans (Ruttner-Kolisko, 1977; Dumont et al., 1975).
Calculations and data analyses
All statistical analyses were run in the statistical program R (version 4.0.5), with a significance level of P < 0.05 (R Core Team, 2021). Most data are presented as the mean and the standard deviation. When DIN and SRP concentrations were below approximately 100 and 10 µg l−1, the reservoirs were considered N or P-limited (Chorus & Spijkerman, 2021), respectively. Phytoplankton RFGs with relative biomass greater than 50% of the total phytoplankton biomass were classified as dominant, while abundant species presented relative biomass greater than 5% of the total biomass. The biomass of herbivorous zooplankton was calculated as the sum of rotifers, calanoid copepods, nauplii, and cladocerans.
To designate the habitat template for each RFG, we selected seven of the eight environmental variables used by Reynolds (2006) (except soluble reactive silica): mixing zone depth, irradiance, water temperature, pH (instead of CO2 concentration), herbivorous zooplankton biomass (instead of herbivorous zooplankton filtration rate), SRP, and DIN. We also incorporated three new variables that are important drivers of phytoplankton structure: water transparency, salinity, and depth. These factors can successfully represent the gradients of resources, energy, loss processes due to zooplankton grazing, and salinity. Mean values of each environmental variable were weighted by each RFG biomass to verify niche differentiation among the RFGs and create the habitat templates, following the assumptions of Southwood (1977). Weighted means were calculated by multiplying all values of each variable by the biomass of a specific RFG, and then the sum of these results was divided by the sum of the biomasses in all samples. After that, we selected the minimum and maximum values of each environmental variable in the samples where each RFG reached relative biomass greater than 5%. However, in the case of any group that did not present a relative biomass > 5%, or only in one sample, we extracted the minimum and maximum values from all occurrences of this group. This approach allowed us to identify the specific range of conditions where each RFG can present higher biomasses.
To better illustrate the habitat templates, we clustered the reservoirs based on the biomass of the RFGs to select the habitats where they can coexist. For that, we constructed a non-metric multidimensional scaling (NMDS) ordination based on the Bray–Curtis distance matrix of RFGs, in the vegan package (Oksanen et al., 2018). We repeated this procedure with the habitat template matrix (weighted means), clustering the RFGs instead of the samples using a Euclidean distance matrix. Then, the slopes of SRP, mixing zone depth, and salinity were extracted to represent the gradients of resources, energy, and salinity, respectively, using the function envfit. The habitat template matrix was standardized using the range function, while the RFGs’ biomass matrix was transformed (log(x + 1)).
Finally, generalized additive models (GAM) were used to verify the influence of each environmental factor on RFGs. This method accounts for linear and non-linear relationships between variables. The biomass of each RFG, mixing zone, irradiance, SRP, DIN, and salinity, were log(x + 1)-transformed. Model smoothing was evaluated using the estimated degree of freedom (e.d.f.), while R2-adjusted and p-values (P < 0.05) indicated model fit and significance, respectively. All models were fitted using the gam function in the mgcv package (Wood, 2004, 2011).
Results
Reynolds Phytoplankton Functional Groups
A total of 136 phytoplankton taxa were identified for the 10 reservoirs studied, distributed mainly in Chlorophyta (51 spp.), Cyanobacteria (40 spp.), and Bacillariophyta (19 spp.), while the others were classified into Dinophyta, Euglenophyta, Cryptophyta, and Chrysophyta (26 spp.). All taxa were included in 20 RFGs, of which 16 were abundant in at least one sample. The mean phytoplankton biomass varied greatly in each reservoir, ranging from 1.88 mg l−1 (standard deviation: ± 1.1) in Cursaí reservoir to 132.75 mg l−1 (standard deviation: ± 100.5) in Tapacurá reservoir (Fig. S1).
Group M was dominant in Tapacurá, Cajueiro, Carpina, Mundaú, Cursaí, and Serrinha reservoirs. Group LO was dominant in Cachoeira, Goitá, and Mundaú, while LM was dominant in Cajueiro, Cursaí, Serrinha, and Carpina. Serrinha reservoir also showed a dominance of the group SN. Finally, Tabocas and Ipojuca reservoirs presented the dominance of the groups F and D, respectively. Groups C, H1, J, K, MP, P, S1, X1, X2, and Y were abundant at least in one sample (> 5%), while the groups E, NA, W1, and W2 always presented low relative biomass (< 5%) (Fig. S1, Table 1).
What lives where, why, and how? Describing and explaining habitat templates of RFGs
To test niche differentiation among the RFGs and create the habitat templates, the mean values of 10 environmental variables were weighted by each RFG biomass (Table 2). The mean mixing zone depth to maximum depth ratio weighted by RFGs’ biomass revealed that groups LM, MP, S1, W2, and P usually occurred in destratified water bodies (i.e., Zmix:Zmax > 0.8), while groups D, W1, LO, and H1 preferred stratified environments (Zmix:Zmax < 0.5). Groups D, W1, LO, J, and Y occurred in reservoirs with low irradiance (< 400 µmol m−2 s−1), while groups F, LM, E, M, and MP presented higher biomass in environments with higher irradiance (> 700 µmol m−2 s−1). Mean water temperature weighted by RFGs’ biomass was lower for RFGs NA and F (< 26 ºC) and higher for RFGs LO, X1, M, and MP (> 28 ºC). Groups D, W1, H1, Y, K, S1, X2, SN, and J were usually found in turbid waters (water transparency < 0.5 m), while groups E, F, and NA preferred clear reservoirs (water transparency > 1.0 m). Groups D and W1 usually occurred in shallow reservoirs (< 3 m), and groups X1, E, F, LM, and P preferred deep reservoirs (> 8 m) (Table 2).
In terms of the amount of resources, groups F and NA were found in SRP-limited reservoirs (< 15 µg l−1), while groups X1, M, MP, S1, and D presented higher biomasses in reservoirs with high SRP concentrations (> 150 µg l−1). For DIN concentration, groups LO and D occurred in DIN-limited waters (< 100 µg l−1), while NA, Y, J, H1, MP, and K showed higher biomasses under high DIN concentrations (> 300 µg l−1). Regarding pH values, groups F, NA, and E preferred neutral waters (pH 7 – 8), while all other groups were common in alkaline habitats (pH > 8). Regarding salinity, groups F, E, P, NA, M, LM, MP, and SN were present in freshwater environments, showing low mean values weighted by the biomass (< 0.5 PSU); on the other hand, groups D and W1 were indicators of saline reservoirs (> 3 PSU), while the other groups were usually found in brackish waters (0.5–3 PSU). In terms of the biomass of herbivorous zooplankton, group F preferred environments with low biomass of herbivores (< 30 µg l−1); on the other hand, all other groups, except X2, LM, K, and J, presented higher biomasses in reservoirs with high herbivorous zooplankton biomass (> 100 µg l−1), especially LO (> 290 µg l−1) (Table 2).
Based on the habitat template, we established the boundaries of environmental variables where RFGs can become abundant (or occur for the RFGs E, MP, NA, W1, and W2) (Fig. 1). These boundaries can act as the thresholds where these groups can grow and present considerable biomasses. The RFGs D, X1, and P presented the most restrictive occurrences as abundant, while RFGs M and SN displayed the widest.
The studied reservoirs were divided into six groups based on the structure of RFGs and similar environmental conditions (Figs. 2a,b). Accordingly, the RFGs were also clustered into six groups based on the means of each variable weighted by the RFG biomass, which also represented the clusters of reservoirs (Figs. 2c,d), besides the resources (SRP) (Fig. 2e), energy (mixing zone depth) (Fig. 2f), and salinity (Fig. 2g) gradients. The first axis of the NMDS separated the gradients of energy and salinity. The water transparency, irradiance, depth, and mixing zone depth increased the biomass of cluster 3 (RFGs LM and P). Cluster 2 (RFGs F, E, and NA) was positively correlated with water transparency, irradiance, and DIN. In the salinity gradient, cluster 6 (RFGs D and W1) was common in saline reservoirs. On the other hand, the second axis of the NMDS represented the resource gradient, separating DIN and SRP + pH on opposite sides. Clusters 1 (RFG LO) and 4 (RFGs S1, MP, M, and X1) were mainly positively influenced by the gradients of SRP, herbivorous zooplankton, temperature, and pH. The latter cluster was also positively influenced by depth and mixing zone. Finally, cluster 5 (RFGs Y, K, X2, J, H1, W2, C, and SN) was influenced by moderate levels of DIN and salinity.
The six groups of reservoirs presented contrasting environmental parameters and phytoplankton communities (Fig. S2). In summary, the first group (Goitá and Cachoeira) was represented by eutrophic, deep, and stratified (low Zmix:Zmax) reservoirs, with high temperature, water transparency, and herbivorous zooplankton biomass, neutral pH, in addition to low irradiance and salinity. The second group (Tabocas) was represented by deep, mesotrophic, and stratified (low Zmix:Zmax) reservoirs, with high irradiance, water transparency, neutral pH, in addition to low herbivorous zooplankton biomass, SRP, DIN, and salinity. Similarly, the third group (Cursaí) showed oligotrophic conditions, was destratified most of the time, with high depth, irradiance, water transparency, neutral pH, low herbivorous zooplankton biomass, SRP, DIN, and salinity (Fig. S2).
The fourth group (Carpina) was hypereutrophic, deep, destratified (Zmix:Zmax = 1), with high water transparency, temperature, pH, SRP, and salinity (moderately saline), besides low herbivorous zooplankton biomass. In addition, the fifth group (Cajueiro—mesotrophic, Tapacurá, Mundaú, and Serrinha—hypereutrophic) was represented, in general, by alkaline conditions, low to moderate irradiance, low herbivorous zooplankton biomass in Mundaú and Serrinha, and high in Tapacurá and Cajueiro, low water transparency and salinity. Tapacurá presented the highest concentrations of SRP, and Mundaú had the highest concentrations of DIN. Finally, the sixth group (Ipojuca) was shallow, saline, hypereutrophic, stratified (low Zmix:Zmax), and alkaline, with low herbivorous zooplankton biomass, besides showing the lowest values of irradiance and water transparency (Fig. S2).
When and where in new scenarios? Predicting the responses of RFGs to environmental changes
Generalized additive models were useful for clearly describing the relationships among RFGs and the 10 environmental variables (Fig. 3, Figs. S3–S12, Table S2). Groups C, M, MP, S1, and SN were positively influenced by pH, and negatively by water transparency. Furthermore, higher pH boosted the growth of groups H1, LM, and X1, while it negatively impacted group F. Irradiance negatively impacted groups D, J, LO, and W1, while water temperature negatively influenced groups F, K, and NA. The herbivorous zooplankton biomass positively influenced only group LO (Fig. 3, Figs. S3–S12, Table S2).
Besides that, groups C, H1, J, K, and Y showed a significant correlation with shallow, saline, stratified (low mixing depth), and turbid conditions. Group X2 was further negatively impacted by higher water transparency. Moreover, groups D and W1 were also associated with shallow, stratified, and saline environments. Group F was associated with extremely low values of salinity (< 0.05 PSU), while group M and SN showed a positive relationship with salinity until 0.5 PSU and decreased markedly in biomass after this value; however, group SN showed a positive relationship again after 2 PSU, while group X2 presented higher biomasses at approximately 2.5 PSU. Depth further negatively influenced groups S1, SN, and W2 (Fig. 3, Figs. S3–S12, Table S2).
Concentrations of DIN positively influenced only groups NA and Y. Concentrations of SRP positively influenced groups M, MP, S1, and X1, besides showing an opposite relationship with groups E and F. The SRP also increased the biomass of groups D and W2 until concentrations of approximately 100 µg L−1 and negatively impacted them at extreme values. Only group P did not correlate with any of the environmental variables (Fig. 3, Figs. S3–S12, Table S2).
In general, salinity was the best predictor of the RFGs’ biomass (higher R2), accounting for 98.8% of the variance of the RFG D, 92% for X2, 89.2% for W1, 78.3% for K, 72.4% for F, 61.3% for C, and 48.6% for Y. Depth was the second-best predictor (60.6% for K, 51.6% for Y, 36.5% for C, and 33.7% for J), followed by SRP (51.9% for E, 39% for X1, and 36.9% for W2), irradiance (37.6% for D), water transparency (37.5% for S1, and 31.4% for SN), and DIN (34.7% for Y). Mixing depth, water temperature, herbivorous zooplankton, and pH always explained less than 30% of the variance in each RFG’s biomass (Fig. 3, Figs. S3–S12, Table S2).
Discussion
What lives where, why, and how? Habitat template and driving processes
The habitat template of most RFGs was constructed primarily based on samples from the temperate region. When this classification was proposed, much less information was available for tropical regions (e.g., Reynolds, 1998, 2006; Reynolds et al., 2002). More recently, Salmaso et al. (2015) graphically represented the habitat template of the RFGs based on data from Reynolds et al. (2002). They statistically confirmed the relationships of the RFGs with environmental variables. However, more information about tropical habitats is needed. Accordingly, in this study, we defined the habitat template for 20 RFGs in tropical drinking water reservoirs, describing the main environmental conditions where they are most successful and can form blooms. Moreover, we designated the boundaries of 10 environmental variables where RFGs can become dominant in tropical reservoirs. These boundaries can act as assembly rules of phytoplankton, delimitating the limits of their occurrences. According to Keddy & Weiher (1999), assembly rules are “values and domain of factors that either structure or constrain the properties of ecological assemblages”. So, those rules must be explicit and quantitative values of key factors, instead of merely describing a pattern (Keddy & Weiher, 1999; Rojo, 2021), as the most descriptive studies did until now.
In our approach, we classified all phytoplankton populations into RFGs, even the non-abundant ones (relative biomass < 5%), unlike most studies (e.g., Costa et al., 2016; Rodrigues et al., 2018; Braga & Becker, 2020; Kruk et al., 2021). Non-dominant species can act as the inoculum of the future assemblages, because, even at low biomasses, they can compete for resources better than dominant species under certain circumstances (Rojo & Álvarez-Cobelas, 2003). Thus, they must not be underestimated (Rojo, 2021). Nevertheless, how the input of a new inoculum affects phytoplankton assembly has not been evaluated yet (Kruk et al., 2021). By considering abundant and non-abundant groups, our approach is also useful for predicting how environmental changes will affect the structure of future phytoplankton assemblages, and thus researchers can estimate when and where phytoplankton RFGs will be more successful under new scenarios of global change.
From the 20 RFGs identified in our study, groups E, NA, W1, and W2 did not present enough biomass to be considered abundant. However, they were present in unique conditions that allowed their growth and tolerance to environmental pressures. If these conditions become more intense, these groups will probably present their best fitness, achieving higher biomasses and possibly becoming dominant in future assemblages. Moreover, what defines the successful development of a phytoplankton species is not its biomass, but how it tolerates environmental inadequacies when it arrives in a new habitat (Reynolds, 2012). Accordingly, evaluating the habitat template and environmental drives of non-dominant groups or species is important in predicting the state and structure of future assemblages.
Based on that, we were able to separate the reservoirs into six main groups of habitat templates. These clusters better reflected the environmental conditions, as well as the RFGs they hosted and their responses to the resource, energy, and salinity gradients. This is the first attempt to recreate the habitat templates of RFGs since their proposal and based on new data. Previously to this article, Salmaso et al. (2015) statistically illustrated the environmental tolerances of 30 RFGs using data from Reynolds’ experiments in temperate lakes. They clustered RFGs into five groups, which were distributed in two main gradients of tolerance: temperature/grazing + DIN, and SRP/irradiance + carbon dioxide. Moreover, the tolerances to low mixing depth and silica concentration were not related to RFG patterns (Salmaso et al., 2015). Even being based on environmental preferences (weighted means of environmental variables) instead of tolerance levels, our analyses revealed different gradients that regulated the patterns of phytoplankton assemblages in tropical reservoirs (resource: DIN/SRP + pH, and energy/salinity gradients).
In the resource gradient, clusters 1 (RFG LO) and 4 (RFGs S1, MP, M, and X1) were associated with alkaline, SRP-rich, and DIN-deficient waters, while cluster 2 (RFGs NA, E, F) correlated with higher concentrations of DIN, lower SRP and neutral pH. In the energy/salinity gradient, clusters 2 and 3 (RFGs LM and P) were related to deep and destratified freshwaters, with high transparency and irradiance. The opposite was found for cluster 6 (RFGs D and W1), which was related to shallow, saline, turbid, and stratified waters with low irradiance. Although cluster 5 (RFGs Y, X2, K, J, C, H1, W2, and SN) was placed at the centre of the NMDS, it tended to be associated with moderate salinities and DIN concentrations. Herbivorous zooplankton and temperature showed a lower influence on RFG structure, but they were important to explain the occurrence of the RFGs LO and NA, respectively. The lower influence of these variables on phytoplankton assembly had already been demonstrated for tropical reservoirs. Considering that zooplankton communities from tropical lakes and reservoirs are usually represented by small organisms (Ger et al., 2016; Amorim et al., 2019), they present lower effects on phytoplankton assemblages (Amorim & Moura, 2020; Amorim et al., 2020), which are in turn well adapted to prevent for being grazed by zooplankton (Wilson et al., 2006).
In contrast to the results found by Salmaso et al. (2015), our habitat template representation, associated with GAM models, showed a stronger effect for mixing zone depth than temperature. This can represent an important difference between tropical and temperate climates. Due to warmer climates, tropical lakes tend to be stratified for longer periods (Brasil et al., 2016), but with similar temperatures throughout the year (Kosten et al., 2009), which can reduce the effects of temperature on phytoplankton assemblages and increase the contribution of stratification in tropical lakes and reservoirs (Amorim et al., 2020).
Furthermore, we also registered the inverse influence of DIN and SRP on phytoplankton assemblages, which can also be a climate-related effect that differs from Salmaso et al. (2015). For instance, higher temperatures, and consequent stratification, can induce a higher release of phosphorus from the sediments, especially in shallow lakes (Jeppesen et al., 2020). On the other hand, the denitrification processes, and consequent loss of nitrogen, are higher with rising temperatures (Herrman et al., 2008). So, considering that the SRP gradient was coupled with temperature in the NMDS analysis, higher concentrations of SRP, associated with low values of DIN, are expected for tropical lakes and reservoirs.
Salinity was the main predictor of ecological changes in phytoplankton assemblages in the reservoirs studied herein. Although salinity has been recognized as one of the main drivers of aquatic organisms in warmer regions, especially in drylands (Barbosa et al., 2020), it was not considered in the original proposal of RFGs’ habitat templates (Kruk et al., 2021). Until certain levels, it is recognized that salinity can stimulate cyanobacterial blooms (Amorim et al., 2020), which can coexist with other phytoplankton assemblages, including diatoms, dinoflagellates, and green algae; and thus, reducing water quality, biodiversity, and ecosystem functioning (Amorim & Moura, 2021). So, it is also important to further study the effects of salinity on phytoplankton in large gradients, capturing the main effects on community assembly and ecosystem functioning.
Additionally, we were able to redraw the habitat templates of 20 RFGs with data exclusively from the tropical region and after the inclusion of three new environmental variables (Fig. 4, Table 3). Most of the habitat templates proposed in this study match with the original description of those proposed by Reynolds (Reynolds, 1998, 2006; Reynolds et al., 2002; Padisák et al., 2009). The exceptions are: RFG E and X1, which were registered mostly in deep reservoirs (instead of shallow); RFG H1, which was abundant in nitrogen-rich waters (instead of nitrogen-limited); RFG P was registered in oligotrophic to hypereutrophic reservoirs (instead of only eutrophic); and RFG LM, that presented higher biomass in oligotrophic to hypereutrophic conditions (instead of eutrophic to hypereutrophic) (Table 3).
The RFG LM was recognized as in an intermediate trophic state group between LO and M (Padisák et al., 2009); however, in this study, LM and M occurred as abundant in oligotrophic to hypereutrophic reservoirs, while LO occurred in mesotrophic to hypereutrophic waters. According to Reynolds (2006), RFG LM tolerates stratification and is sensitive to mixing; nevertheless, in our study, this group was present usually in deep destratified reservoirs, while LO occurred in warm stratified waters. This result can be explained by the lower relative biomass of Ceratium furcoides (Levander) Langhans in Microcystis-dominated systems (always < 10%, except in one sample from Cursaí reservoir), which might suggest that RFG LM is more related to M, instead of being an intermediate between LO and M in tropical lakes and reservoirs. Likewise, Ceratium and Microcystis showed different dominance patterns in another tropical Brazilian lake, the Garças Pond, without stable coexistence (mandatory to recognize the LM assemblage) (Crossetti et al., 2019). So, more research is needed to verify if Ceratium and Microcystis can coexist in tropical lakes, or if this pattern is seen only in temperate and subtropical environments.
When and where in new scenarios? RFGs as predictors of environmental change
Since its proposal, the RFG approach has been largely used to describe spatiotemporal patterns of phytoplankton and its tolerance and sensitivity to environmental variables (Salmaso et al., 2015). However, much less has been done to explain “why and how” phytoplankton is assembled, and more research should focus on explaining “when and where” phytoplankton RFGs will dominate under new scenarios of environmental change (Kruk et al., 2021).
Two main modelling approaches can be used to predict phytoplankton dynamics and bloom formation, which include process-based and data-driven models (Rousso et al., 2020). Currently, PROTECH (Phytoplankton RespOnses To Environmental CHange) is one of the most common models used to predict phytoplankton structure, allowing the simulation of daily changes in the biomass of different taxa (Reynolds et al., 2001; Elliott, 2021). However, these models require many variables and a huge dataset, which are not always available. Alternatively, data-driven models can be useful for predicting phytoplankton response to changes in environmental conditions based on a preexistent dataset (Rousso et al., 2020). As shown above, in our study, GAM models (a data-driven approach) were efficient in predicting the response of 20 RFGs to 10 environmental gradients, providing explanations of the mechanisms involved in their dominance in specific ranges of environmental conditions (habitat templates). Based on that, we quantified the relationships between RFGs’ biomass and each environmental variable for dominant, abundant, and non-abundant groups, which enabled the prediction of the most favourable set of environmental conditions considered optimal for their growth in the resource, energy, and salinity gradients (Fig. 6).
Accordingly, the ability of RFGs to predict environmental change can support the adoption of sustainable practices to avoid eutrophication. In this regard, the knowledge of the key functional traits of dominant phytoplankton groups is essential for the success of management strategies to control algal blooms (Ibelings et al., 2016). Based on that, Mantzouki et al. (2016) developed a schematic framework to inform lake managers of the best practices to control the main RFGs responsible for harmful cyanobacterial blooms. The combination of these practices will maintain the provision of ecosystem services in lakes and reservoirs, allowing the sustainable development and conservation of aquatic resources. Coupled with the information about habitat templates, those management strategies can be helpful to understand and control algal blooms in tropical drinking water reservoirs, where they are supposed to impact water quality, plankton diversity, structure, and ecosystem functioning (Amorim & Moura, 2021).
Conclusions
To the best of our knowledge, this is the first attempt to recreate the habitat template of RFGs exclusively for tropical aquatic ecosystems. We successfully designated six sets of habitat templates, based on the common RFGs and the environmental conditions where they are most successful. Furthermore, generalized additive models were useful for explaining and predicting the structure of phytoplankton, with most of the variance in the RFGs’ biomass explained by salinity, depth, SRP, irradiance, transparency, and DIN. As our sampling included only the superficial layer of the reservoirs, the habitat templates proposed here should be considered only for epilimnetic phytoplankton assemblages.
Our approach does not substitute the habitat templates proposed by Reynolds et al. (2002) and Padisák et al. (2009) but complements the understanding of how RFGs interact with environmental gradients in tropical reservoirs, by considering dominant and non-dominant groups and including three new environmental variables. Based on our results, water managers can predict in which circumstances RFGs will become dominant in tropical drinking water lakes and reservoirs. With this knowledge, it will be possible to anticipate the need for restoration measures. Although we described the structure of RFGs, their main processes, and mechanisms in six sets of habitat templates, some of them are under-represented and still need further clarifications. As Reynolds (2006) said: “the ecology of populations and communities is relevant to many aspects of human existence, from the safety of drinking water to the sustainability of fisheries. The accumulated knowledge is both broad and deep but it is far from complete”. So, more research is needed, especially to explain and predict phytoplankton structure in tropical habitats, including lakes, reservoirs, rivers, estuaries, and oceans.
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Acknowledgements
We thank the Limnology Laboratory and Professor William Severi, from the Department of Fisheries and Aquaculture of the Federal Rural University of Pernambuco, for supporting nutrient analysis.
Funding
This work was supported by the Brazilian National Council of Technological and Scientific Development—CNPq, Brazil (Grant Number 305829/2019–0), and Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco—FACEPE, Brazil (Grant Number IBPG-0308–2.03/17).
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CAA participated in the conceptualization, methodology development, validation, data curation, and formal analysis of all data, wrote the original draft, wrote, reviewed, and edited the final version of the manuscript. ANM participated in the conceptualization, supervision, funding acquisition, methodology of all data, wrote, reviewed, and edited the final version of the manuscript. All authors read and approved the final manuscript.
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Amorim, C.A., Moura, A.d.N. Habitat templates of phytoplankton functional groups in tropical reservoirs as a tool to understand environmental changes. Hydrobiologia 849, 1095–1113 (2022). https://doi.org/10.1007/s10750-021-04750-3
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DOI: https://doi.org/10.1007/s10750-021-04750-3