Abstract
Amylosucrase (AS), a glucosyltransferase of the glycoside hydrolase 13 family, does not require a nucleotide-activated sugar as a glucosyl-donor. A gene (drpas) of Deinococcus radiopugnans encoding a putative AS was identified and cloned for characterization. The amino acid sequence revealed that drpas exhibited 76 and 74% identities with AS genes from D. geothermalis and D. radiodurans, respectively. Recombinant DRpAS exhibited AS activities. The ratios of hydrolysis, polymerization, and isomerization reactions were 5.8:82.7:11.5 at 40°C. The DRpAS was highly thermostable and produced more polymerization products than AS from D. radiodurans although the optimum growth temperature of both strains is 30°C.
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Kim, MD., Seo, DH., Jung, JH. et al. Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans . Food Sci Biotechnol 23, 2007–2012 (2014). https://doi.org/10.1007/s10068-014-0273-3
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DOI: https://doi.org/10.1007/s10068-014-0273-3