Abstract
One of the most important aspects of bioinformatics is locating genes within a long DNA sequence. Until the development of bioinformatics, the only way to locate genes along the chromosome was to study their behavior in the organism or isolate the DNA and study in a test laboratory. Bioinformatics allow scientists to make educated guesses about where genes are located simply by analyzing sequence data using a computer. The CRISPR/CAS9 system has been rapidly adopted for genome editing. In a typical CRISPR, a SgRNA is designed to have a guide sequence domain designated. However, one major time with this system is the lack of robust bioinformatics tools for design of single guide RNA which determines the efficiency and specificity of genome editing.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
https://ib.bioninja.com.au/standard-level/topic-3-genetics/32-chromosomes/identifying-genes.html
Genetics: https://medlineplus.gov/genetics/
Gregory A D, Rios M, Reid ME (2000) Part 3—protocols. In: Denomme GA, Rios M, Reid ME (eds) Molecular protocols in transfusion medicine. Academic Press, pp 19–65. ISBN 9780122093708
Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS, Weis AM, Voytas DF, Grunwald DJ (2012) Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome. PLoS Genet 8(8):e1002861
Harrow J, Nagy A, Reymond A et al (2009) Identifying protein-coding genes in genomic sequences. Genome Biol 10:201
Fujita H, Fujita T, Fujii H (2021) Locus-specific genomic DNA purification using the CRISPR system: methods and applications. The CRISPR J
Cavanagh and Garrity (2014) “CRISPR Mechanism”, CRISPR/Cas9, Tufts University. https://sites.tufts.edu/crispr/
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Kiranmai, B., Vasantha, S.V., Jyoshna, B., Suvarna Mukhi, B. (2023). Detection of Accurate Gene Location for Modification or Replacement of DNA and True Guide Synthetic gRNA for CRISPR. In: Kumar, A., Ghinea, G., Merugu, S. (eds) Proceedings of the 2nd International Conference on Cognitive and Intelligent Computing. ICCIC 2022. Cognitive Science and Technology. Springer, Singapore. https://doi.org/10.1007/978-981-99-2746-3_49
Download citation
DOI: https://doi.org/10.1007/978-981-99-2746-3_49
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-99-2745-6
Online ISBN: 978-981-99-2746-3
eBook Packages: Intelligent Technologies and RoboticsIntelligent Technologies and Robotics (R0)