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CRISPR Guide RNA Design Guidelines for Efficient Genome Editing

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RNA Tagging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2166))

Abstract

The simple applicability and facile target programming of the CRISPR/Cas9-system abolish the major boundaries of previous genome editing tools, making it the tool of choice for generating site-specific genome alterations. Its versatility and efficacy have been demonstrated in various organisms; however, accurately predicting guide RNA efficiencies remains an organism-independent challenge. Thus, designing optimal guide RNAs is essential to maximize the experimental outcome. Here, we summarize the current knowledge for guide RNA design and highlight discrepancies between different experimental systems.

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Authors and Affiliations

  1. Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany

    Patrick Schindele, Felix Wolter & Holger Puchta

Authors
  1. Patrick Schindele
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  2. Felix Wolter
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  3. Holger Puchta
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Corresponding author

Correspondence to Holger Puchta .

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Editors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes (IBMP-CNRS), Université de Strasbourg, Strasbourg, France

    Manfred Heinlein

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Schindele, P., Wolter, F., Puchta, H. (2020). CRISPR Guide RNA Design Guidelines for Efficient Genome Editing. In: Heinlein, M. (eds) RNA Tagging. Methods in Molecular Biology, vol 2166. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0712-1_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0712-1_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0711-4

  • Online ISBN: 978-1-0716-0712-1

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