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Amplified Fragment Length Polymorphism: Applications and Recent Developments

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2222))

Abstract

AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes. It is more efficient in terms of time, economy, reproducibility, informativeness, resolution, and sensitivity, compared to other popular DNA markers. Besides, it requires very small quantities of DNA and no prior genome information. This technique is widely used in plants for taxonomy, genetic diversity, phylogenetic analysis, construction of high-resolution genetic maps, and positional cloning of genes, to determine relatedness among cultivars and varietal identity, etc. The review encompasses in detail the various applications of AFLP in plants and the major advantages and disadvantages. The review also considers various modifications of this technique and novel developments in detection of polymorphism. A wet-lab protocol is also provided.

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Authors and Affiliations

  1. Indian Institute of Spices Research, Kozhikode, Kerala, India

    Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari & Kantipudi Nirmal Babu

  2. Division of Crop Improvement and Biotechnology, ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India

    Thotten Elampilay Sheeja

  3. Providence Women’s College, Kozhikode, Kerala, India

    Divakaran Minoo

  4. Central Plantation Crops Research Institute, Kasaragod, Kerala, India

    Muliyar Krishna Rajesh

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  1. Thotten Elampilay Sheeja
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  1. UMR PVBMT, Universite de la Reunion, St Pierre, Réunion, France

    Pascale Besse

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Sheeja, T.E., Kumar, I.P.V., Giridhari, A., Minoo, D., Rajesh, M.K., Babu, K.N. (2021). Amplified Fragment Length Polymorphism: Applications and Recent Developments. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 2222. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0997-2_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0996-5

  • Online ISBN: 978-1-0716-0997-2

  • eBook Packages: Springer Protocols

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