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Probing Vasculature by In Vivo Phage Display for Target Organ-Specific Delivery in Regenerative Medicine

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Vascularization for Tissue Engineering and Regenerative Medicine

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

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Abstract

Different mammalian tissues in their normal and pathological state have distinct molecular features in the luminal side of the vessels. These features can be targeted with peptides that concentrate, i.e., home to their target tissue. The homing peptides can be used to deliver cargo, such as therapeutic molecules, to the target tissue, or they can help in detecting pathological changes in the tissue. Some of the homing peptides are capable of penetrating to the target tissue and bringing their cargo with them. One of the most studied homing peptides, iRGD, penetrates to its target tissue with the help of the CendR sequence. The homing and penetrating peptides are discovered by screening a peptide library encoded on the surface of bacteriophages. In in vivo biopanning, the library is injected to the circulation of a living animal, and later, unbound phages are washed off during circulation and bound phages are eluted from the target tissue. The eluted phages are amplified and form the library for the second biopanning round. After a few rounds, the phages containing sequences that home to the target tissue are concentrated in the library.

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Acknowledgments

TP wants to thank MD Antti Pemmari for his insightful comments. This work was supported by the Academy of Finland and Emil Aaltonen foundation.

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Authors and Affiliations

  1. Tampere University, Tampere, Finland

    Toini Pemmari, Tiia Koho & Tero A. H. Järvinen

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  1. Toini Pemmari
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  2. Tiia Koho
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  3. Tero A. H. Järvinen
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Correspondence to Tero A. H. Järvinen .

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Editors and Affiliations

  1. Austrian Cluster for Tissue Engineering, Vienna, Austria

    Wolfgang Holnthoner

  2. Department of Biomedicine, University Hospital Basel, Basel, Switzerland

    Andrea Banfi

  3. Institute for Pathology, Johannes Gutenberg University, Mainz, Germany

    James Kirkpatrick

  4. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Austrian Cluster for Tissue Regeneration, Wien, Austria

    Heinz Redl

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Pemmari, T., Koho, T., Järvinen, T.A.H. (2021). Probing Vasculature by In Vivo Phage Display for Target Organ-Specific Delivery in Regenerative Medicine. In: Holnthoner, W., Banfi, A., Kirkpatrick, J., Redl, H. (eds) Vascularization for Tissue Engineering and Regenerative Medicine. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-54586-8_21

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