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Seeing Is Believing: Noninvasive Microscopic Imaging Modalities for Tissue Engineering and Regenerative Medicine

  • Living reference work entry
  • First Online:
Cell Engineering and Regeneration

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

Abstract

Microscopy has made a significant impact on science and is today an integral part of a researcher toolbox and techniques to test scientific hypothesis. However, only recently has the possibility to observe important biological interactions and mechanisms deep inside a tissue, with the ability to focus on a single cell, emerged. New technological discoveries have resulted in broadening the focus of this standard method and promoted novel modalities to be used in several biological contexts and to validate scientific hypothesis. Nowadays, noninvasive methods have shown interesting and groundbreaking applications in tissue engineering, tumor biology, and immunology. Multiphoton microscopy (MPM), second harmonic generation (SHG), fluorescence lifetime imaging microscopy (FLIM), Förster resonance energy transfer (FRET), and intravital microscopy (IVM) have harnessed both endogenous and exogenous fluorophores to uncover new molecular and cellular biology pathways while enabling single cell tracking. By means of these techniques, it is now possible to unveil extracellular matrix density and alignment, perform metabolic analysis, observe complex macromolecular interactions, and validate cell division, death, communication, and fate. Within this chapter, we highlight and showcase the versatility and applicability of different microscopic approaches and their qualitative and quantitative insight into cellular and molecular biology, tissue engineering, immunology, metabolism, and tumor biology.

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Authors and Affiliations

  1. Department of Mechanical and Manufacturing Engineering and Trinity Centre for Bioengineering, Trinity College Dublin, Dublin 2, Ireland

    Nuno Neto & Michael G. Monaghan

  2. Metabolic Imaging Group, School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland

    Ruslan I. Dmitriev

  3. Advanced Materials for BioEngineering Research (AMBER) Centre, Trinity College Dublin and Royal College of Surgeons in Ireland, Dublin 2, Ireland

    Michael G. Monaghan

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  1. Nuno Neto
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  2. Ruslan I. Dmitriev
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  3. Michael G. Monaghan
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Correspondence to Michael G. Monaghan .

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  1. Tulane Univ. Sch. of Medicine, New Orleans, LA, USA

    Jeffrey M. Gimble

  2. AUVA Center, Ludwig Boltzmann Institute for Experimen, Wien, Wien, Austria

    Darja Marolt Presen

  3. Human Development and Health, University of Southampton, Southampton, UK

    Richard Oreffo

  4. Experimental and Clinical Traumatol, Ludwig Boltzmann Institute for, Wien, Austria

    Heinz Redl

  5. AUVA Center, Ludwig Boltzmann Institute for Experimen, Vienna, Austria

    Susanne Wolbank

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Neto, N., Dmitriev, R.I., Monaghan, M.G. (2020). Seeing Is Believing: Noninvasive Microscopic Imaging Modalities for Tissue Engineering and Regenerative Medicine. In: Gimble, J., Marolt Presen, D., Oreffo, R., Redl, H., Wolbank, S. (eds) Cell Engineering and Regeneration. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-37076-7_40-1

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