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Isolation of Natural Products by Low-Pressure Column Chromatography

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Natural Products Isolation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 864))

Abstract

This chapter describes the isolation of natural products using low-pressure column chromatography (LPLC) and provides a brief summary of the adsorption and size exclusion processes involved in LPLC. Different types of stationary phases used in both adsorption and size exclusion LPLC are also presented with specific examples. Various operational parameters in relation to column packing (both wet and dry), column equilibration, sample application, and column development, and a summary of generic procedures for adsorption and size exclusion LPLC are also incorporated in this chapter. Throughout the chapter, several specific examples of the application of LPLC for the isolation of various natural products including alkaloids, coumarins, flavones, and saponins from various plants are given. Many of these examples include a combination of adsorption and size exclusion chromatography to obtain specific compounds.

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Authors and Affiliations

  1. School of Pharmacy, The Robert Gordon University, Aberdeen, UK

    Raymond G. Reid

  2. Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, WV11LY, UK

    Satyajit D. Sarker

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  1. Raymond G. Reid
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Correspondence to Raymond G. Reid .

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Editors and Affiliations

  1. School of Applied Sciences, Department of Pharmacy, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, United Kingdom

    Satyajit D. Sarker

  2. , Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, United Kingdom

    Lutfun Nahar

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Reid, R.G., Sarker, S.D. (2012). Isolation of Natural Products by Low-Pressure Column Chromatography. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_7

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  • DOI: https://doi.org/10.1007/978-1-61779-624-1_7

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