Summary
A gradient HPLC-charged aerosol detection method was applied to the measurement of different lipids including: free fatty acids, fatty alcohols, glycerides, steroids, phospholipids, and fat-soluble vitamins. An algal oil sample is used as an example. Charged aerosol detection offers a new approach to the routine analysis of any nonvolatile lipid. It shows low ng (on column) sensitivity, a dynamic range of over four orders of magnitude, good reproducibility, gradient compatibility, and similar analyte response for nonvolatile species, independent of chemical structure. Furthermore, charged aerosol detection uses mobile phases that are fully compatible with LC–MS – enabling both detectors to be used in a “lipidomics platform.”
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Fahy, E., Subramaniam, S., Brown, A., Glass, C., Merrill, A., Murphy, R., Raetz, C., Russell, D., Seyama, Y., Shaw, W., Shimizu, T., Spencer, F., van Meer, G., VanNieuwenhze, M., White, S., Witztum, J., and Dennis, E. (2005). A comprehensive classification system for lipids. J. Lipid Res., 46, 839–861.
Watson, A. (2006). Lipidomics: A global approach to lipid analysis in biological systems. J. Lipid Res., 47, 2101–2110.
Stryer, L. (2006). Biochemistry, 6th Edition, W.H. Freeman and Co., New York.
Acworth, I. N. (2003). The Handbook of Redox Biochemistry. CD-ROM. ESA Biosciences. Part number: 70–6090.
Wenk, M. (2005). The emerging field of lipidomics. Nat. Rev. Drug Discov., 4, 594–610.
Seppanen-Laakso, T., and Oresic, M. (2008). How to study lipidomes. J. Mol. Endocrinol. In press.
van Meer, G., (2005). Cellular lipidomics. EMBO J., 24, 3159–3165.
Wenk, M.R. (2005) The emerging field of lipidomics. Nature, 4, 594–610.
Morreau, R. (2006). The analysis of lipids via HPLC with a charged aerosol detector. Lipids, 41, 727–734.
Cascone, A., Eerola, S., Ritieni, A., and Rizzo, A. (2006). Development of analytical procedures to study changes in the composition of meat phospholipids caused by induced oxidation. J. Chromatogr. A, 1120, 211–220.
Ramosa, R.G., Libonga, D., Rakotomangab, M., Gaudina, K., Loiseaub, P.M., Chaminade, P. (2008). Comparison between charged aerosol detection and light scattering detection for the analysis of Leishmania membrane phospholipids. J. Chromatogr. A, 1209, 88–94.
Liu, X-K., Fang, J.B., Cauchon, N., Zhou, P.. (2008). Direct stability-indicating method development and validation for analysis of etidronate disodium using mixed-mode column and charged aerosol detector. J. Pharm. Biomed. Anal., 46, 639–644
Nair, L.M., Werling, J.O. (2009). Aerosol based detectors for the investigation of phospholipid hydrolysis in a pharmaceutical suspension formulation. J. Pharm. Biomed. Anal., 49(1), 95–99.
Snyder, L.R., Kirkland, J.J., Glajch, J.L. (1997). Practical HPLC Method Development, Second Edition, Wiley, New Jersey.
Cardozo, K.H.M., Guaratini, T., Barros, M.P., Falcao, V.R., Tonon, A.P., Lopes, N.P., Campos, S., Torres, M.A., Souza, A.O., Colepicolo, P., Pint, E. (2007). Metabolites from algae with economical impact. Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol. 146(1,2), 60–78.
Crafts, C., Bailey, B., Plante, M., Acworth, I. (2009). Evaluation of method for the simultaneous analysis of cations and anions using HPLC with charged aerosol detection and a zwitterionic stationary phase. J. Chromatogr. Sci. In Print.
Miroslav, L., Lynen, F, Hoľcapek, M., and Sandra, P. (2007). Quantitation of triacylglycerols from plant oils using charged aerosol detection with gradient compensation. J. Chromatogr. A, 1176, 135–142.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Plante, M., Bailey, B., Acworth, I. (2009). The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_23
Download citation
DOI: https://doi.org/10.1007/978-1-60761-322-0_23
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-321-3
Online ISBN: 978-1-60761-322-0
eBook Packages: Springer Protocols