Abstract
Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states. In this chapter, detailed procedures for establishing and maintaining primary cultures of adult human dermal fibroblasts are described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Alberts B, Johnson A, Lewis J et al (2002) Fibroblasts and their transformations: the connective-tissue cell family. In: Molecular biology of the cell, 4th edn. Garland Science, New York
Biedermann T, Bottcher-Haberzeth S, Klar AS, Widmer DS, Pontiggia L, Weber AD, Weber DM, Schiestl C, Meuli M, Reichmann E (2015) The influence of stromal cells on the pigmentation of tissue-engineered dermo-epidermal skin grafts. Tissue Eng Part A 21(5–6):960–969
Klar AS, Biedermann T, Michalak K, Michalczyk T, Meuli-Simmen C, Scherberich A, Meuli M, Reichmann E (2017) Human adipose mesenchymal cells inhibit melanocyte differentiation and the pigmentation of human skin via increased expression of TGF-betal. J Invest Dermatol 137(12):2560–2569
Yamaguchi Y, Hearing VJ, Itami S, Yoshikawa K, Katayama I (2005) Mesenchymal-epithelial interactions in the skin: aiming for site-specific tissue regeneration. J Dermatol Sci 40(1):1–9
Driskell RR, Watt FM (2015) Understanding fibroblast heterogeneity in the skin. Trends Cell Biol 25(2):92–99
Lynch MD, Watt FM (2018) Fibroblast heterogeneity: implications for human disease. J Clin Invest 128(1):26–35
Philippeos C, Telerman SB, Oules B, Pisco AO, Shaw TJ, Elgueta R, Lombardi G, Driskell RR, Soldin M, Lynch MD, Watt FM (2018) Spatial and single-cell transcriptional profiling identifies functionally distinct human dermal fibroblast subpopulations. J Invest Dermatol 138(4):811–825
Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA (2002) Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 3(5):349–363
Darby IA, Hewitson TD (2007) Fibroblast differentiation in wound healing and fibrosis. Int Rev Cytol 257:143–179
Eyden B (2005) The myofibroblast: a study of normal, reactive and neoplastic tissues, with an emphasis on ultrastructure. Part 2—tumours and tumour-like lesions. J Submicrosc Cytol Pathol 37(3–4):231–296
Gabbiani G (2003) The myofibroblast in wound healing and fibrocontractive diseases. J Pathol 200(4):500–503
Biedermann T, Boettcher-Haberzeth S, Reichmann E (2013) Tissue engineering of skin for wound coverage. Eur J Pediatr Surg 23(5):375–382
Klar AS, Zimoch J, Biedermann T (2017) Skin tissue engineering: application of adipose-derived stem cells. Biomed Res Int 2017:9747010
Wood FM, Kolybaba ML, Allen P (2006) The use of cultured epithelial autograft in the treatment of major burn injuries: a critical review of the literature. Burns 32(4):395–401
Braziulis E, Diezi M, Biedermann T, Pontiggia L, Schmucki M, Hartmann-Fritsch F, Luginbuhl J, Schiestl C, Meuli M, Reichmann E (2012) Modified plastic compression of collagen hydrogels provides an ideal matrix for clinically applicable skin substitutes. Tissue Eng Part C Methods 18(6):464–474
Pontiggia L, Biedermann T, Meuli M, Widmer D, Bottcher-Haberzeth S, Schiestl C, Schneider J, Braziulis E, Montano I, Meuli-Simmen C, Reichmann E (2009) Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. J Invest Dermatol 129(2):480–490
Klar AS, Guven S, Biedermann T, Luginbuhl J, Bottcher-Haberzeth S, Meuli-Simmen C, Meuli M, Martin I, Scherberich A, Reichmann E (2014) Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells. Biomaterials 35(19):5065–5078
Boettcher-Haberzeth S, Klar AS, Biedermann T, Schiestl C, Meuli-Simmen C, Reichmann E, Meuli M (2013) “Trooping the color”: restoring the original donor skin color by addition of melanocytes to bioengineered skin analogs. Pediatr Surg Int 29(3):239–247
Boettcher-Haberzeth S, Biedermann T, Pontiggia L, Braziulis E, Schiestl C, Hendriks B, Eichhoff OM, Widmer DS, Meuli-Simmen C, Meuli M, Reichmann E (2013) Human eccrine sweat gland cells turn into melanin-uptaking keratinocytes in dermo-epidermal skin substitutes. J Invest Dermatol 133(2):316–324
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kisiel, M.A., Klar, A.S. (2019). Isolation and Culture of Human Dermal Fibroblasts. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_6
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9473-1_6
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9472-4
Online ISBN: 978-1-4939-9473-1
eBook Packages: Springer Protocols