Abstract
Cell therapy is one of the most hopeful technologies of regenerative medicine approaches. Among various cells, human skin fibroblasts have been progressively used for wound healing as cell-based therapy purposes. By increasing the age, the number of skin fibroblasts’ abilities including cell migration, growth, collagen production, etc. decreases. Hence, use of the fetal source is more beneficent. In this respect, this chapter covers the manufacturing of human fetal skin-derived fibroblasts for clinical application.
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Abbreviations
- bFGF:
-
Basic fibroblast growth factor
- CBMPs:
-
Cell-based medicinal products
- CD:
-
Cluster of differentiation
- cGMP:
-
Current good manufacturing practice
- CPMP:
-
Committee for Proprietary Medicinal Product
- CVMP:
-
Committee for Medicinal Products for Veterinary Use
- DMEM-LG:
-
Dulbecco’s Modified Eagle’s Medium-low glucose
- DMSO:
-
Dimethyl sulfoxide
- EMA:
-
European Medicines Agency
- FBS:
-
Fetal bovine serum
- HBV:
-
Hepatitis B virus
- HCV:
-
Hepatitis C virus
- HEPA:
-
High efficiency particulate air
- HIV:
-
Human immunodeficiency virus
- kGy:
-
Kilogray
- LMP:
-
Last menstrual period
- PBS:
-
Phosphate-buffered saline
- Pen-Strep:
-
Penicillin-Streptomycin
- RPR:
-
Rapid plasma reagin
- RT-PCR :
-
Reverse transcription polymerase chain reaction
- SOP:
-
Standard operating procedure
- TSE:
-
Transmissible spongiform encephalopathy
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Acknowledgments
The authors would like to thank Dr. Mohsen Khorshidi, Shokouh Salimi, Maryam Afshari, and Ali Valadkhani for their considerable assistance.
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Goodarzi, P. et al. (2019). Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_233
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DOI: https://doi.org/10.1007/7651_2019_233
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0250-8
Online ISBN: 978-1-0716-0251-5
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