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FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells

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Genomic Mosaicism in Neurons and Other Cell Types

Part of the book series: Neuromethods ((NM,volume 131))

Abstract

Genomic or chromosomal mosaicism in human brain cells is considered a source for neuronal diversity and a mechanism for neuropsychiatric diseases. However, there is still a lack of consensus concerning the extent and effects of mosaic chromosome abnormalities (i.e., aneuploidy) in the normal and diseased human brain. To solve this problem, a need for detailed description of single-cell techniques for chromosomal analysis of human brain cells appears to exist. In this chapter, FISH-based techniques for detecting genomic (chromosomal) mosaicism in the human brain are described.

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Acknowledgments

Professor YB Yurov is supported by a grant from the Russian Science Foundation (project #14-35-00060) at Moscow State University of Psychology and Education. Professors SG Vorsanova and IY Iourov are supported by a grant from the Russian Science Foundation (project #14-15-00411) at Mental Health Research Center. The study of the Alzheimer disease brain is supported by the ERA.Net RUS Plus program.

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Authors and Affiliations

  1. Mental Health Research Center, 34, Kashirskoye shosse, Moscow, 117152, Russia

    Yuri B. Yurov, Svetlana G. Vorsanova, Ilia V. Soloviev, Alexei M. Ratnikov & Ivan Y. Iourov

  2. N.I. Pirogov Russian National Research Medical University, Academician Yu.E. Veltishchev Research Clinical Institute of Pediatrics, Ministry of Health of the Russian Federation, Moscow, Russia

    Yuri B. Yurov, Svetlana G. Vorsanova & Ivan Y. Iourov

  3. Moscow State University of Psychology and Education, Moscow, Russia

    Yuri B. Yurov, Svetlana G. Vorsanova & Alexei M. Ratnikov

  4. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Moscow, Russia

    Ivan Y. Iourov

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  1. Yuri B. Yurov
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  2. Svetlana G. Vorsanova
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  3. Ilia V. Soloviev
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  4. Alexei M. Ratnikov
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  5. Ivan Y. Iourov
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Correspondence to Ivan Y. Iourov .

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Editors and Affiliations

  1. Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute (IC-CSIC), Madrid, Spain

    José María Frade

  2. Biological Studies and Laboratory of Genetics, The Salk Institute, La Jolla, California, USA

    Fred H. Gage

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Yurov, Y.B., Vorsanova, S.G., Soloviev, I.V., Ratnikov, A.M., Iourov, I.Y. (2017). FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells. In: Frade, J., Gage, F. (eds) Genomic Mosaicism in Neurons and Other Cell Types. Neuromethods, vol 131. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7280-7_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7280-7_2

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